YSI 2730 User Manual Download

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1725 Brannum Lane
Yellow Springs, Ohio 45387 USA

027424

937 767-7241

•

800 765-4974

•

Fax 937 767-9353

A27424C

[email protected]

•

www.YSI.com

May 00



1998 YSI Incorporated

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Summary of Contents for 2730

Page 1: ... excess underutilized and idle equipment along with credit for buybacks and trade ins Custom engineering so your equipment works exactly as you specify Critical and expedited services Leasing Rentals Demos In stock Ready to ship TAR certified secure asset solutions Expert team I Trust guarantee I 100 satisfaction All trademarks brand names and brands appearing herein are the property of their resp...

Page 2: ... 6 5 63 Monitor and Control Accessory 8VHU V 0DQXDO Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 3: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 4: ...etup 15 5 2 1 Monitor 15 5 2 2 Antiseptic 15 5 2 3 Sterilization 16 5 2 4 Make Up Cycle 16 5 2 5 Autocalibration 16 5 2 6 Analog Control 17 5 3 Service 20 5 3 1 Monitor 20 5 3 2 Analog Control 20 6 PID Control 20 6 1 1 Assumptions 20 7 Printed Setup Information 21 8 Service 22 8 1 Tubing Replacement 22 8 1 1 Pump Tubing Replacement 22 8 1 2 Solenoid Valve Tubing Replacement 23 8 2 External Chamber...

Page 5: ...ings 9 Figure 7 Output Polarity 10 Figure 8 Handshake Signals 12 Figure 9 Software Flow Chart 14 Figure 10 Minimum Purge Time 15 Figure 11 Installing Pump Tubing 22 Figure 12 Schematic 1 27 Figure 13 Schematic 2 28 Figure 14 Monitor Pump Solenoid Assembly 30 Figure 15 External Chamber Assembly 31 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 6: ...s is sufficient to purge the external sample chamber and deliver fresh sample The flow rate of the pump and the purge time can be varied and is programmed via the 2700 software The Solenoid Valve assembly allows multiplexing between the sample stream and an antiseptic stream This preserves sterility by filling the end of the sampling line with an antiseptic after every sample The analog output sec...

Page 7: ...OD x 0 035 ID inches Valve Tubing 0 03 ID inches Wasteline Tubing Silicone 0 16 OD x 0 10 ID inches Nominal Flow Rate inlet line 100 2500 microliters minute 8 6 PSI Analog Control Full Scale Voltage Selectable 10 00 VDC or 5 00 VDC Full Scale Concentration User selectable via software as 1 2 3 or 4 x Calibrant Concentration Resolution 1 4096 or 0 02 FS 2 44 mv on 10 00 VFS 1 22 mv on 5 00 VFS Maxi...

Page 8: ...ernally via the Ack signal input Ack input Active low resets Ready output minimum width 10ms SysErr output Active High indicates 2700 not ready to sample Filtrate output external sample pump control BLK Pump output feed diluent pump control for Black Probe Chemistry WHT Pump output feed diluent pump control for White Probe Chemistry Artisan Technology Group Quality Instrumentation Guaranteed 888 8...

Page 9: ...rposes of compatibility To install the accessory a small flat head screwdriver a 2 Phillips screwdriver needle nose pliers and a 5 16 or small adjustable wrench are needed The steps are as follows 1 Turn off instrument power using the switch on the rear of the 2700 case Do not unplug the power cord 2 Remove the two screws on the upper rear of the instrument to allow the hinged back panel to open O...

Page 10: ...ligned gently press it into its socket 1 J13 R76 X2 R77 C16 C2 R78 C40 C19 C32 SW1 R55 R52 D24 R49 D27 D29 D28 D26 C81 U31 C80 U2 U3 U35 U33 U34 C64 C65 J15 LED D14 D13 1 J9 C29 U16 U19 R27 R32 R33 R26 Q1 Q6 Q5 Q4 U25 D15 D19 D20 D17 D18 D16 D9 D12 D11 D10 1 J8 1 J12 1 J11 U24 U23 U21 U22 U4 U15 U20 U26 U32 C77 C78 C79 U17 U28 C88 C38 C76 C83 C85 C84 C82 C75 C37 C31 C43 C49 C62 C56 C63 C59 C60 C67...

Page 11: ...ide plate Save the locknuts 12 Install the Monitor Pump Solenoid Valve Assembly from the inside by positioning the two studs through the Pump Valve Mounting Plate Be certain that the solenoid valve is above the pump Refer to Figure 4 Reuse the two 5 16 locknuts that were removed above and secure the Pump Valve Assembly 13 Plug the Pump Motor lead six conductor into the socket marked J4 on the 2730...

Page 12: ...ble waste container not included 17 Close the rear panel door and replace the two screws which hold it Be careful not to snag any cables when closing the door 18 Install the supplied label on the back of the instrument to indicate that it is equipped with the 2730 hardware This is intended to prevent others from using the instrument with the turntable doing so would damage the 2730 circuits 19 Ins...

Page 13: ...he Sipper Tube with the External Sampling Chamber and to prime the External Pump Purge Pump 25 Select 3 External Chamber to move the Sipper Tube to Station 5 external chamber Now adjust the Chamber Bracket forward or backward to align the Sipper and the Thumb Screw to adjust the chamber side to side Visually align the Sipper over the conical opening to the External Chamber 26 Now test the alignmen...

Page 14: ...ration for the polarity of the Black White and Filtrate Pump Control Outputs is normal not inverted See the following sections if you have special Analog Control Output requirements 3 2 1 Full Scale Voltage The Digital to Analog Converters on the YSI 2730 circuit board may be individually configured to provide either 5 volts or 10 volts full scale The JP1 jumper positions 3 through 6 control this ...

Page 15: ...LECT automatically samples a bioreactor process stream or other suitable sample source Since color turbidity optical density and many other physical factors do not affect the YSI enzyme biosensor filtration and or dilution may not be necessary and the 2700 2730 may draw the sample directly If cell loss is a concern or if high cell density is expected a filtration device e g tangential flow filter ...

Page 16: ...11 4 1 2 Examples Monitor Only Monitor with 2730 Control Monitor with Computer Control Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 17: ...te before the next sample is ready Figure 8 shows the typical signal pattern that would occur during two sample update cycles The READY signal will reset itself immediately prior to updating the analog outputs if not reset externally via the ACK signal input 4 2 2 Pump Control Outputs Three discrete outputs are provided on the YSI 2730 which are intended to control external pumps The means of cont...

Page 18: ...ump Control 12 Red Power Out 13 Blue Ack 14 White Black Ready 15 Orange Chassis Ground None Shield 81 DVLF 2SHUDWLRQ IMPORTANT The following instructions were written assuming a familiarity with the YSI 2700 SELECT Biochemistry Analyzer If you ordered the 2700 and 2730 together and are setting up for the first time you should refer to the 2700 SELECT USER S MANUAL for instructions Once you have su...

Page 19: ...sections of the 2700 menu structure Figure 9 shows the new structure with the 2730 features in bold lettering The following section briefly describes the 2730 menu options Figure 9 Software Flow Chart Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 20: ...chamber and system tubing This is especially important when an antiseptic is used since the antiseptic may damage the enzyme membrane if aspirated by the sipper needle Figure 10 shows the flow rates and minimum time needed to purge the tubing lines from the solenoid valve to the external sample chamber These values are based upon a 6 fold volume turnover Additional time may be necessary to purge t...

Page 21: ...led by the user the solenoid valve still switches to the antiseptic position and air or fluid is pumped through the antiseptic line during the make up cycle The antiseptic line must be kept free from obstructions even if the antiseptic cycle is not used A dry 0 2 micron filter may be inserted upstream from the solenoid valve to prevent contamination in the air from entering the antiseptic line dur...

Page 22: ...ameters allow you to control pumps that will feed additional nutrients or diluent to a reactor in order to achieve a desired set point Black or White Channel Setup The channel control setup menus prompt users for four pieces of information the analyte set point Time Per Unit error TPU the method of control error direction and the full scale concentration of each analog output The first three param...

Page 23: ...smallest volume 2 NOTE Time Per Unit error in seconds per unit of measure liter e g 50 seconds gram liter Example You are trying to regulate a 5 0 Liter bioreactor at 1 00 g L glucose concentration The actual volume of broth in the reactor is maintained at 3 5 Liters You know from previous fermentations of that the maximum consumption rate of glucose is approximately 28 5 g L hr The feed pump deli...

Page 24: ...e effect of diluting or removing analyte to perform regulation Example In the case of a fermentation where glucose is the controlled analyte the user should select the under error direction since the organisms in the fermentation will consume glucose thereby forcing the controller to add glucose via feed stock additions 4 Analog Output The voltage output of the analog interface is scaled relative ...

Page 25: ...ated into the 2730 software is a variant of the Proportional Integral Derivative PID algorithm used in many process controllers today Because of the many factors which can affect control it is difficult if not impossible to accommodate all circumstances in a single form of the PID control algorithm The 2730 control algorithm is no exception Certain assumptions apply to the application of this cont...

Page 26: ...e black control set point is 1 00 g L and the white control set point is 0 INSTRUMENT SETUP Sample Size 25 uL SamStation 2 CalMethod One station BLACK PROBE Chemistry Dextrose Unit g L Calibrator 2 50 Endpoint 30 Sec CalStation 1 WHITE PROBE Chemistry Dextrose Unit g L Calibrator 2 50 Endpoint 30 Sec CalStation 1 AUTOCAL SampleError ON Temperature 1 C Time 15 Min Sample 5 Sam Cal Shift 2 RUN MODE ...

Page 27: ...ing the pump assembly to the pump motor sideplate Next lower the pump body to a working surface remove the two O rings holding the pump together and separate the two halves of the pump body Then remove the roller assembly noting the slot in one end of the center shaft of the assembly This slot must engage with the pump motor shaft protruding through the 2700 SELECT case wall Be certain to orient t...

Page 28: ...ructions below 1 From the 2700 main menu press MENU 3 DIAGNOSTIC 2 PUMP then 4 EXTERNAL PUMP This will energize the solenoid and engage the pump 2 Install the Sample Tubing in the outer solenoid valve slot Make sure that the tubing lies completely at the bottom of the slot Do not stretch the tubing 3 Press 4 EXTERNAL PUMP to stop the pump and de energize the solenoid 4 Install the Anti septic Tubi...

Page 29: ...function and or tubing obstructions Refer to Section 8 Troubleshooting in the 2700 SELECT USER S MANUAL as an extension of this troubleshooting section Before taking corrective action related to any problem be certain to collect as much pertinent data as possible Try to make use of the 2730 service and diagnostic routines to test potential problem areas If you cannot resolve a problem contact YSI ...

Page 30: ...ng If fluid flows the obstruction is in the chamber or the waste line If fluid does not flow try massaging the tubing to try relieving the blockage If unsuccessful begin disconnecting tubing and forcing air or fluid through with a syringe until you locate the blockage SYMPTOM ________________________________________________________________ Bubbles appear at the top of the 2730 External Chamber whe...

Page 31: ...OM ________________________________________________________________ Sipper aspirates antiseptic instead of sample sample results read approximately zero POSSIBLE CAUSE Tubing routed incorrectly in solenoid valve CORRECTIVE ACTION Check tubing routing per Section 8 1 2 NOTE Replace membrane if damaged by antiseptic FOR PROBLEMS NOT IDENTIFIED IN THE ABOVE CHART CONTACT YSI TECHNICAL SUPPORT FOR ASS...

Page 32: ...D LP2951 U4 8 1 6 7 4 2 5 3 R R 10K RN1 1 9 1 2 3 4 5 6 HEADER JP1 1 2 3 4 5 6 7 8 9 10 11 12 DAC A DAC B DB0 AD1 AD0 DB7 DB6 DB5 DB4 DB3 DB2 DB1 WR CS LD VREF VSS AGND DGND REFINB REFINA VOUTB OFSTB VOUTA OFSTA VDD AD7237 U2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 100uF C7 1 2 0 01uF C6 1 2 3 3uF C5 2 1 R R 10K RN1 1 8 R R 10K RN1 1 7 R R 10K RN1 1 6 W_PUMP AD0 AD2 AD3 AD4 ...

Page 33: ...5pf C9 1 2 10M R8 1 2 10K R4 1 2 25pf C8 1 2 1K R5 1 2 HC04 SPARE U7 8 9 HC04 SPARE U7 2 1 J3 1 10K RN1 1 4 1UF C13 1 2 10K RN1 1 3 HC04 U7 4 3 SPARE 10K RN1 1 10 10K RN1 1 2 J4 4 HC04 U7 6 5 O1 O2 O3 O4 O5 O6 O7 O8 VM GND I8 I7 I6 I5 I4 I3 I2 I1 ULN2803 U5 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 PH3 SOL_DRV PH3_OUT PH2_OUT PH0_OUT B_PUMP PH1_OUT PH0 PH1 SOL_DRV W_OUT F_OUT PH2 ANTI_STEP SAM_...

Page 34: ...1 Tubing solenoid 1 32 5 110433 Motor assy 6 USE 2731 Tubing PharMed 035 ID 7 USE 2731 Tubing waste silicone 156 OD 4 8 USE 2731 Fitting Y Kynar 1 16 ID 1 9 USE 2731 Fitting straight Kynar 1 16 ID 3 10 002550 Washer Nylon 141 x 312 x 031 2 11 001710 Screw Mach 6 32 x 375 2 12 002680 Washer lock 8 328 x 020 4 13 001740 Screw Mach 6 32 x 375 4 14 027420 Thumbscrew 2 15 USE 2731 O ring 301 x 070 2 N ...

Page 35: ...30 Figure 14 Monitor Pump Solenoid Assembly Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 36: ...2 023024 Compression Spring 3 110440 Chamber 4 001008 Thumb Screw 5 061994 Thumb Nut 6 061700 Outlet Fitting 10 32 3 32 hose 7 069818 Flat Washer 6 550 x 031 Figure 15 External Chamber Assembly Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 37: ...rence to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures Reorient the receiving antenna Relocate the computer with respect to the receiver Move the computer away from the receiver Plug the computer into a different outlet so that the computer and receiver are...

Page 38: ...or parts in servicing or repairing the product or vi modification of the product in any way not expressly authorized by YSI THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE YSI s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE...

Page 39: ...sponsibility of the sender When service is required either at the user s facility or at YSI the following steps must be taken to insure the safety of our service personnel 1 In a manner appropriate to each device decontaminate all exposed surfaces including any containers 70 isopropyl alcohol or a solution of 1 4 cup bleach to 1 gallon tap water are suitable for most disinfecting Instruments used ...

Page 40: ...ound the product completely with packing material 5 Insure for the replacement value of the product Cleaning Certificate Organization Department Address City State Zip Country Phone Model No of Device Lot Number Contaminant if known Cleaning Agent s used Radioactive Decontamination Certified Answer only if there has been radioactive exposure Yes No Cleaning Certified By Name Date Artisan Technolog...

Page 41: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 42: ...Springs Ohio 45387 USA 027424 937 767 7241 800 765 4974 Fax 937 767 9353 A27424C Info YSI com www YSI com May 00 1998 YSI Incorporated Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 43: ...quipment Have surplus equipment taking up shelf space We ll give it a new home Learn more Visit us at artisantg com for more info on price quotes drivers technical specifications manuals and documentation Artisan Scientific Corporation dba Artisan Technology Group is not an affiliate representative or authorized distributor for any manufacturer listed herein We re here to make your life easier How...

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