PV830 Pneumatic PicoPump
World Precision Instruments
1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can
cause damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can
cause personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—PV830 Pneumatic PicoPump
INTRODUCTION
The
PV830
is designed to inject very small quantities of fluids, such as drugs,
into cells or small organelles. Pressure injection is an especially useful alternative
to electroionophoresis, since it does not mandate the use of charged ions. Two
different positive pressures may be applied–one for ejection at high pressure and
a second, lower pressure to prevent back filling of the pipette by capillary action
or diffusion. Vacuum may also be applied to hold cells or small objects and to load
pipettes from the tip. Cells may be held by vacuum and simultaneously injected
using pressure.
Notes and Warnings
!
WARNING: WHEN USING A DELIVERY MICROPIPETTE, SECURE THE
PIPETTE FIRMLY. WHEN HIGH PRESSURE IS APPLIED, A LOOSE PIPETTE
CAN BE EJECTED FORCEFULLY. DO NOT APPLY PRESSURES IN EXCESS
OF 150PSI (1000KPA). USE DRY AIR, NITROGEN OR OTHER INERT GASES ONLY.
!
WARNING: THIS INSTRUMENT IS FOR INVESTIGATIONAL USE ONLY IN
ANIMALS OR OTHER TESTS THAT DO NOT INVOLVE HUMAN SUBJECTS.
