WPI PicoPump PV830 Instruction Manual Download Page 4 | Manualshive

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World Precision Instruments

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Summary of Contents for PicoPump PV830

Page 1: ...PV830 Pneumatic PicoPump World Precision Instruments INSTRUCTION MANUAL Serial No _____________________ 042712 www wpiinc com...

Page 2: ......

Page 3: ...cking 2 INSTRUMENT DESCRIPTION 3 Setup 5 Vacuum Input 6 Pressure Input 6 Eject Pressure Port front panel 6 Vacuum Port 8 OPERATING INSTRUCTIONS 9 Front Panel 9 Rear Panel 10 Techniques In Microinjecti...

Page 4: ...iv World Precision Instruments...

Page 5: ...ally useful alternative to electroionophoresis since it does not mandate the use of charged ions Two different positive pressures may be applied one for ejection at high pressure and a second lower pr...

Page 6: ...any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department...

Page 7: ...position of the Vac Hold toggle switch This feature allows the pressure ejection channel to have a mild sucking action when pressure is not being applied A manual pneumatic switch also allows the vacu...

Page 8: ...or Depress the plastic collar to remove tubing Fig 5 The PicoPump Pressure and Vacuum ports are located on the rear panel of the PV830 To install the hard tubing push it into the port until the tubing...

Page 9: ...asily while metal tubing may require more effort For a diagram of the setup see Fig 6 Air or Nitrogen Supply 150 psi max Vacuum Pump or Line Pressure Input Vacuum Input Hold Eject Port Micropipette Ho...

Page 10: ...o a nitrogen regulator on a N2 tank Recommended gases are dry air nitrogen or argon Never use corrosive gases If an air pressure line containing oil or water vapor is used an external filter is recomm...

Page 11: ...hen using a MPH6S handle a firmer hold on the glass can be achieved by using two gaskets in the micropipette holder NOTE Capillary holders can be obtained from WPI which contain Ag AgCl half cells The...

Page 12: ...ak when attaching or removing the tubing Before inserting the pipette place a gasket of the correct size in the cap Gasket Color Green Black Red White Pipette Diameter mm 1 0 1 2 1 5 1 65 WARNING DANG...

Page 13: ...n dial yielding good pressure resolution The pressure regulating mechanism functions by continuously bleeding a small amount of gas This bleed rate increases as the difference between the input pressu...

Page 14: ...electronic timer controls the time duration that the pressure solenoid stays open and the three commands serve only to start the timer Long pulses in the timed mode may be aborted by pressing the Stop...

Page 15: ...helps you to correctly use the Hold pressure The backfilling flow rate is determined by the pressure of capillary action and the tip size Since the tip size is often determined by the requirement of t...

Page 16: ...s cleaning the glass Small amounts of dust or grease can easily clog micron sized tips Cleaning with chromic acid solutions before pulling the electrode is commonly performed but care must be taken to...

Page 17: ...ation The same technique may be used though if the drop is kept submerged under oil Droplets may seem to disappear after emergence from the tip Sometimes this is due to creepage of the aqueous fluid b...

Page 18: ...approximately 1mV for every 10 PSI of applied pressure Multibarrel Microinjection For injection with multibarrel micropipette the PolyFil multibarrel micropipette coupling kit can be purchased from W...

Page 19: ...e 1 Kit MPH6S for 1 5 mm pipette 5 ft tubing assembly 5430 20 PicoNozzle 1 Kit MPH6S for 2 0 mm pipette 5 ft tubing assembly 5430 ALL PicoNozzle 2 Kit MPH6S Micropipette Holder specify 1 0 1 2 1 5 or...

Page 20: ...port will push the diaphragm and return the O ring to the seat To introduce a high pressure 1 Remove the 1 4 hard pressure tubing from the back of the pump 2 Use pieces of tubing with 1 4 OD to make a...

Page 21: ...ng the knobs Over tightening the knobs will damage the regulators Vacuum Vacuum Input rear 0 30 0 in Hg 101kPa Vacuum Output 0 29 9 in Hg 101kPa Lowest Regulated Vacuum 3 in water 0 7kPa Regulator Acc...

Page 22: ...hysical Specifications Power 95 130 VAC or 190 260 VAC switch selectable single phase 50 60 Hz 20VA Temperature 10 C 50 F to 40 C 104 F Dimensions 3 5 x 17 x 9 5 in 89 x 432 x 242 mm Mountable in stan...

Page 23: ...L 215 m 267 m 1nL 100 m 1 24 m 100pL 46 m 57 m 10pL 21 5 m 27 m 1pL 10 m 12 4 m Fig 13 This graph compares the dimensions of a cube and a sphere with the same internal volume Volume of a Micropipette...

Page 24: ...ion sensitive microelectrodes J Neuroscience Methods 8 231 247 1983 Deyhimi F and J A Coles Rapid silylation of a glass surface Choice of reagent and effect of experimental parameters on hydrophobici...

Page 25: ...PV830 Pneumatic PicoPump World Precision Instruments 21...

Page 26: ...22 World Precision Instruments DECLARATION OF CONFORMITY...

Page 27: ...indirect special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused dir...

Page 28: ...44 0 1462 424700 Fax 44 0 1462 424701 E mail wpiuk wpi europe com Germany Zossener Str 55 10961 Berlin Tel 030 6188845 Fax 030 6188670 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading...

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