10
World Precision Instruments
Sealing O-ring
The conical hole in the center points forward.
This seal must have tension or it could leak.
Aluminum Barrel
Plunger
Plastic Sleeve (spacer)
placed between “O” rings
with indentation toward glass
Front Gasket
Collet
Glass
Push the glass in, then tighten the collet.
The end of the glass must seat firmly in the
gasket here, or it may leak.
Fig. 12—The front gasket can be installed in either direction.
NOTE
: It is essential that these components (O-ring/gaskets) be installed in the
correct orientation to hold the glass micropipette in place and proper functionality of
NANOLITER2020
Injector.
Replacing the Gaskets
To change the gaskets, unscrew the collet and slide the collet assembly off of the wire
plunger. Unscrew the aluminum barrel from the Nanoliter injector. The O-ring should
slide easily from the collet (Fig. 13).
Collet Assembly
O-Ring
Wire Plunger
Injector
Aluminum Barrel
Fig. 13—(Left)The injector is disassembled to replace the gaskets.
Fig. 14— (Right) Use the tweezers to remove the gasket from the aluminum barrel.
When you slide the barrel over the plunger without the collet assembly, the sealing
O-ring (black) and the plastic sleeve (white) should come off. If not, use a small pair of
curved forceps to scoop them out of the end of the barrel (Fig. 14). To reassemble the
unit, slide the barrel over the wire plunger and screw it in place on the injector. DO
NOT bend the plunger. Position the Sealing O-ring and the Plastic Sleeve as described
above. Install the appropriate O-ring on a fresh micropipette and screw the collet in
place. See “Setting up the Injector” on page 9.
Connecting the NANOLITER2020 Injector with the Controller
1. Plug the
NANOLITER2020
cable into one of the
Output
connection ports on the rear
panel of the SMARTouch™ controller (Fig. 15). Make sure the marked arrow on the
connector is facing upwards for proper pin alignment. The controller allows one or
two pumps to be controlled independently or simultaneously.