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WORLD

PRECISION

INSTRUMENTS

NANOLITER2020

MICRO2T SMARTouch™ Controller-Based Nanoliter

2020 Injection

Serial No._____________________

www.wpiinc.com

INSTRUCTION MANUAL

082020

Summary of Contents for NANOLITER2020

Page 1: ...WORLD PRECISION INSTRUMENTS NANOLITER2020 MICRO2T SMARTouch Controller Based Nanoliter 2020 Injection Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 082020...

Page 2: ......

Page 3: ...tting Up the System 9 Cleaning the NANOLITER2020 9 Setting up the Injector 9 Replacing the Gaskets 10 Connecting the NANOLITER2020 Injector with the Controller 10 OPERATING INSTRUCTIONS 12 Setting Par...

Page 4: ...uter Access 29 Disabling Motors for Low Noise Recording 30 Setting Screen Brightness 30 Resetting System Defaults 30 Displaying Firmware Revision 30 Resetting Limits after Power Failure 31 Grouped Ope...

Page 5: ...he 300704 Injector head can be purchased separately or with the MICRO2T controller INTRODUCTION The NANOLITER2020 Injector works on the principle of positive displacement An internal micro step motor...

Page 6: ...nt Verify that all items are included 1 300704 NANOLITER2020 Injector head with universal adapter 500778 The injector comes with gaskets to use with 1 1 1 15 mm glass installed Items listed below are...

Page 7: ...PTION NANOLITER2020 Description The NANOLITER2020 is the system which includes NANOLITER2020 Injector Head 300704 MICRO2T SmartTouch Controller and the required accessories Two sample micropipettes ar...

Page 8: ...NANOLITER2020 40 mm Fig 2 NANOLITER 2020 System can be used on a micromanipulator which is NOT included with the NANOLITER2020 Universal Adapter Mounting Rod for stereotaxic frame or micromanipulator...

Page 9: ...ht red when the RUN button is pressed and the NANOLITER2020 pump is running O Unit is unplugged Dim Unit has power Bright Unit is running Fig 4 The LED indicator on the NANOLITER2020 Injector Head ind...

Page 10: ...selected pump The syringe delivery sequence is established through the Configuration screen While the pump is running the border of the pump s information display flashes to indicate which pump is ru...

Page 11: ...s information about each pump connected to the controller Fig 8 The pump display area shows vital information for each pump attached to the controller Command screen Fig 8 shows Pump Number is located...

Page 12: ...ction ports and the power switch USB Serial Connection 3 5mm Foot Switch Port Output Connections for UMP3 or Nanoliter 2010 Power Switch Power Supply Port Fig 9 The rear panel of the MICRO2T controlle...

Page 13: ...uld leak Plastic Sleeve spacer placed between O rings with indentation toward glass Front Gaskets 1 1 1 15 mm 1 3 1 35 mm 1 5 mm Sealing O ring The conical hole in the center points forward This seal...

Page 14: ...Plunger Injector Aluminum Barrel Fig 13 Left The injector is disassembled to replace the gaskets Fig 14 Right Use the tweezers to remove the gasket from the aluminum barrel When you slide the barrel o...

Page 15: ...d converts it to 12 V 3 Connect the power cord to the power supply and plug the AC power cord into a wall outlet Fig 17 Fig 17 Connect the power cord to the power supply 4 If the foot switch WPI 13142...

Page 16: ...he appropriate pump channel on the Command screen by tapping the desired channel on the display Fig 18 Channel 1 is selected and PUMP1 is shown with a blue field behind it in the upper right corner of...

Page 17: ...e as close as possible to the target volume based on the selected step microstepping and syringe size This value changes to reflect the effects of changes to the target volume syringe type and motor d...

Page 18: ...dent on the selected syringe and the desired delivery rate Motor Drive lets you set the pump to deliver the aliquot volume using the minimum number of microsteps or the maximum number of microsteps th...

Page 19: ...f the NANOLITER2020 plunger It is important to define the limits of travel before you begin using the pump This keeps the pump from driving past the mechanical limits of the pump and avoids any jammin...

Page 20: ...TALLED CHECK THE END STOP CALIBRATION AGAIN 2 Set Syringe This button is not used for the NANOLITER2020 Injector Setting End Stop in the Nanoliter Injector 1 Power on the MICRO2T unit The splash scree...

Page 21: ...Port 1 of the MICRO2T controller Fig 26 To make a pump active press anywhere in the PUMP Display Area on the Syringe Status screen 4 Press the Configure button to open the Pump Configuration screen Fi...

Page 22: ...pen the Syringe Stop Definition screen Fig 30 The Syringe Stop Definition screen lets you define the limits of travel for the pump Four white navigation buttons in the center of the Syringe Stop Defin...

Page 23: ...er until you see it reach its withdrawn position at the base of the collet where the arrow is pointing in this image 10 When you see that the plunger is properly positioned at the base of the collet 1...

Page 24: ...is how mineral oil is back filled into glass micropipettes Front Filling Front filling describes the filling of a micropipette through the small pulled front end of the pipette Front filling is achie...

Page 25: ...acer The glass end must be firmly seated in the plastic sleeve spacer or it could leak It is absolutely essential that these components be properly assembled Fig 36 Left Slide the blunt end of the mic...

Page 26: ...Head press the CONFIGURE button to access the Pump Configuration screen Set the Volume Target and the Delivery Rate by touching each field and entering the desired value Fig 39 Touch the appropriate...

Page 27: ...rawing Example Parameters Delivery Rate 200 nL sec Volume Target 3000 nL Motor Drive MAX LOAD 7 Press the RUN button This will draw fluid front fill into the micropipette NOTE The backward arrow shows...

Page 28: ...e facing towards the barrel of the pump and the smaller hole facing toward the micropipette 4 Then slide the white plastic sleeve spacer on the plunger with the flat surface side facing towards the ba...

Page 29: ...bly 3 Using the 0 035 hex wrench provided with the replacement plunger loosen the two set screws Fig 47 Loosen the set screws with the hex wrench 4 Remove the plunger 5 Insert the new plunger and tigh...

Page 30: ...e the Nanoliter 2020 injector or controller The control box may be cleaned by wiping it with a clean damp cloth ACCESSORIES Part Number Description 300746 Spare Parts Kit includes MicroFil MF34G displ...

Page 31: ...stops Set the direction away from the end of travel if the syringe is at the stop Plunger fails to retract completely The home position is set incorrectly Reset the End Stop See Setting End Stop in th...

Page 32: ...4 nL sec Recommended Glass TIP10XV119 Pre Pulled Micropipette 504949 Glass capillary 1 14 mm OD Fire Polished Glass Glass Use Capabilities 1 10 1 15 mm OD Fire Polished Glass with Green Front gasket 1...

Page 33: ...RO2T unselect the Sound Enabled check box To re enable the controller sounds select the Sound Enabled check box Press Back to save the setting and bring up the Command screen Enabling Remote Computer...

Page 34: ...drive motors are always enabled when the MICRO2T is powered up The only time the motors are disabled is when the Low Noise Recording check box is selected and the System Options screen is displayed Se...

Page 35: ...pumps Until the limits are reset the message continues to display on power up To reset the end stop see Calibrating the MICRO2T System Travel Limits on page 15 Grouped Operations Pumps may be grouped...

Page 36: ...multiple injections Both pumps will begin delivery of their sequences Each pump will complete delivery of its sequence unless it reaches a mechanical limit or the master pump reaches a mechanical limi...

Page 37: ...9 Set active pump to infuse direction I Set active pump to withdraw direction W Start delivery from stopped condition or resume from paused G Halt volume delivery STOP H Cancels delivery in process P...

Page 38: ...Pause script execution A is length of pause in seconds 100 Response PAUSING while in pause OK when finished pause Beep F is length of beep in seconds 100 Response BEEP while beeping OK when finished b...

Page 39: ...704 Injector Head cannot be used with WPI s old standard controller Can I use same MICRO2T controller that I use with UMP3 pump Do I need an additional adapter to use the 300704 pump with my existing...

Page 40: ...7 injecting sample 22 install micropipette 21 L leakage 24 LED 5 low noise recording 30 M M3301 3 Manual button 7 max load 14 MF34G 5 2 26 MICRO2T 2 5 26 35 MicroFil 21 micromanipulator 3 micropipette...

Page 41: ...NANOLITER 2020 200704 World Precision Instruments 37...

Page 42: ...38 World Precision Instruments DECLARATION OF CONFORMITY...

Page 43: ...irect special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directl...

Page 44: ...Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong New District Shanghai PR China...

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