Walz MINI-PAM-II, Manual, Page: 51 / 130

Hints Chapter 6
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6 Hints & Troubleshooting
6.1 Instrument Settings
Instrument settings were adjusted at the factory for optimum per-
formance of the MINI-PAM-II. For example, LED currents have
been adjusted to meet target PAR values for the measuring set-
up of the 60° distance clip or the 2035-B leaf clip (Fig. 3 and Fig.
5). For a different geometries, the internal PAR sensor requires
recalibration to correctly measure internal actinic light (see Fig.
50, page 87). Estimation of PAR according to Fig. 4 (page 12) is
rather inaccurate.
Also, the fluorescence offset (F-Offset) of your system has been
measured and was saved on the MINI-PAM-II memory. That
means that the MINI-PAM-II should show a fluorescence value
close to zero with the fiber fully inserted and in the absence of a
sample. If this signal deviates clearly from zero, newly adjust F-
Offset (see Fig. 39, page 72).
6.2 Default settings
For fluorescence measurements with most healthy green leaves,
default settings for measuring light and saturation pulses are well
suited. Some samples, however, require special settings. The
following sections will provide some hints to adjust settings
properly.