Walz MINI-PAM-II, Manual, Page: 37 / 130

Saturation Pulse Analysis Chapter 5
31
5 Saturation Pulse Analysis
5.1 Five Fluorescence Levels
Usually, five different levels of modulated fluorescence are ac-
quired by PAM fluorometers. Two of these levels (F
0
and F
M
)
must be measured with the dark-acclimated sample. The three
other levels (F
0
’, F, and F
M
’) are measured with the actinic light-
exposed sample or in a dark period following this light treatment
(see Fig. 22, page 31). Some parameters of saturation pulse
analysis require fluorescence measurement of the same sample
Fig. 22: Measurements for Saturation Pulse Analysis. AL, Actinic Light; D,
dark; SP, Saturation Pulse; FR, Far red illumination.