| Instruction manual
24
2
1
Slider position
Empty
DF
Meaning
empty hole
darkfield ring
Application
Brightfield observation
Darkfield observation with
4x,10x, 20x objectives
11. Use of the microscope in
darkfield (transmitted light)
11.1 Darkfield slider
1
Move the slider into empty position 1 to perform brightfield
observation. (Fig. 47)
2
Move the slider into position “DF” 2 to perform darkfield
observation.
3
When observing in darkfield, keep the aperture diaphragm
adjustment lever on the “O” (open) position.
Darkfield observation is possible with objectives having N.A.
up to 0.40.
With higher N.A. the darkfield effect cannot be achieved
because of the condenser N.A.
FIGURE 47