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Instruction manual | 

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21

In brightfield, optimum observation is generally possible by 

setting the aperture diaphragm to between 70% and 80% of 

the numerical aperture of the objective.
 In phase contrast (PH) or in darkfield (DF), the aperture 

diaphragm must be fully opened by operating on the AS lever.

APERTURE 

DIAPHRAGM

FIELD OF VIEW

30-20%

70-80%

FIGURE 41

Summary of Contents for avantor 600 Series

Page 1: ...VERSION 1 0 ISSUED 28 02 2021 Model European Catalogue Number IT600 FLD 630 3267 VWR Microscope VisiScope Series 600 Instruction manual...

Page 2: ...CTURER Europe VWR International bv Researchpark Haasrode 2020 Geldenaaksebaan 464 B 3001 Leuven 32 16 385011 be vwr com UK Importer VWR International Ltd Hunter Boulevard Magna Park Lutterworth Leices...

Page 3: ...erts 16 9 9 Illumination column 17 9 9 1 Using colour filters 17 9 9 2 Tilting the condenser holder 17 9 9 3 Rotating the condenser holder 17 9 9 4 Centering the LED 18 9 9 5 ECO button 18 9 9 6 Cente...

Page 4: ...responsibility deriving from incorrect instrument use that does not comply with this manual 2 Safety information Avoiding electrical shock Before plugging in the power supply make sure that the suppl...

Page 5: ...upply 13 Power cord 14 Green filter IF550 15 Blue filter LBD 16 Dust cover 17 Holder for Petri diam 38 mm 18 Holder for Terasaki and Petri diam 65 mm 19 Holder for slide and Petri diam 54 mm 20 Lens c...

Page 6: ...ds one around the neck and one around the base lift the microscope from the container and put it on a stable desk 5 Intended use For research and teaching use only Not intended for any animal or human...

Page 7: ...ht intensity indicator 7 Eco button 8 Brightness adjustment knob transmitted light 9 Tension adjustment collar 10 Coarse focus knob 11 Fine focus knob 12 Main switch 13 X y stage movement knobs 14 Obj...

Page 8: ...site side 1 2 3 4 5 6 7 1 Diffusion filter 2 Fluorescence led lamp housing 3 Fluorescence filter selector 4 Brightness adjustment knob reflected light 5 Fluorescence filter indicator 6 Reflected light...

Page 9: ...ectives regularly In inverted microscopes the objectives are very sensitive to dust To prevent dust and contamination from entering the microscope cover all the unused holes with dust caps 3 Fig 3 Whe...

Page 10: ...tage Fig 5 7 1 3Installing stage inserts When using holder metal or glass stage plates make sure that the plate is horizontal 1 Install the stage insert in the stage opening Fig 6 2 Install the holder...

Page 11: ...der inserting the pin on the rear side of the condenser with the alignment groove in the condenser holder Fig 10 2 Lock the condenser with the locking knob 1 on the right side of the condenser holder...

Page 12: ...nsert the lamp housing in the fluorescence illuminator connector in the back side of the microscope Fig 14 3 Tighten the locking screw using the provided Allen screwdriver Fig 15 4 Connect the cable o...

Page 13: ...power supply into the power socket of the microscope Fig 18 3 Plug the power cord into the socket of the power supply Fig 19 4 Plug the power cord into the mains socket Check for a safe connection Ple...

Page 14: ...nto the light path Focus the specimen Adjust field and aperture iris diaphragms Insert desired objective for the observation and focus the spec imen Adjust interpupillary distance Adjust diopters Cent...

Page 15: ...rightness Fig 21 2 While rotating the brightness adjustment knob the LED indicator on the front panel 3 will turn ON or OFF some segments Fig 22 9 3 Adjust the interpupillary distance Observing with b...

Page 16: ...ted on the adjustment ring graduation should correspond to the operator s diopter correction 9 5 Use of eyeshields Use with eyeglasses Fold rubber eyeshields with both hands Folded eyeshields avoid sc...

Page 17: ...ring 1 Fig 28 Clockwise rotation increases the tension If the tension is too loose the revolver could go lower by itself or the focus easily lost after fine adjustment In this case rotate the knob in...

Page 18: ...tage surface it is convenient to tilt the condenser Doing this it is possible to use the ambient light to illuminate the specimen Fig 30 9 9 3 Rotating the condenser holder When using big bottles the...

Page 19: ...filter to perform the LED centering operation and to keep the filter in during normal observation 9 9 5 ECO button 1 Press the ECO button Fig 34 to activate the ECO function Once activated the system...

Page 20: ...cumscribes the field of view in order to eliminate unnecessary light to eyepieces Fig 38 9 9 8 Use of diffusion lens When using 4x objective an additional lens is needed 1 Insert the lens 1 when 4x ob...

Page 21: ...y possible by setting the aperture diaphragm to between 70 and 80 of the numerical aperture of the objective In phase contrast PH or in darkfield DF the aperture diaphragm must be fully opened by oper...

Page 22: ...1 must be used with 4x and 10x phase contrast objectives the 20x 40x position 2 with the 20x and 40x and the SL position 3 is used for brightfield Fig 42 10 2 Centering the phase ring Usually this op...

Page 23: ...g center coincide Fig 46 6 The centering of the slider phase ring and the objective phase ring must be checked with each objective If the phase ring is incorrectly centered the contrast will be severe...

Page 24: ...1 Darkfield slider 1 Move the slider into empty position 1 to perform brightfield observation Fig 47 2 Move the slider into position DF 2 to perform darkfield observation 3 When observing in darkfiel...

Page 25: ...ve into the light path Focus the specimen Begin observation Adjust field iris diaphragm Insert desired objective for the observation and focus the spec imen Adjust interpupillary distance Adjust diopt...

Page 26: ...position the whole mechanism is switched off and therefore the LED will not move when the filtercube selector is moved 1 Switch on the fluorescence illuminator by turning the reflected light intensit...

Page 27: ...t image Set the diaphragm according to the objective in use until it circumscribes the field of view in order to eliminate unnecessary light to eyepieces Fig 52 14 Simultaneous Phase Contrast Fluoresc...

Page 28: ...inocular port then tighten the clamping screw Fig 53 T2 ring is not provided along with the microscope but is commercially available While shooting dark specimens darken eyepieces and viewfinder with...

Page 29: ...ring is not centered Adjust the bolts to center The objective used is not compatible with the phase ring Please use a compatible objective The phase contrast depends on the sample position The sample...

Page 30: ...oscope to manufacturer for maintenance please use the original packaging 18 Technical service Web Resource Visit the VWR s website at www vwr com for Complete technical service contact information Acc...

Page 31: ...It is also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposa...

Page 32: ...14 85 00 international info fr vwr com 0 18 TTC min prix appel POLAND VWR International Sp z o o Limbowa 5 80 175 Gdansk Tel 48 58 32 38 200 info pl vwr com PORTUGAL VWR International Material de Labo...

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