Instruction manual |
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6
Reinstall the filter slider on the microscope.
7
Close the side cover.
8
Apply the adhesive marker (2) for the fluorescence cube on the
side cover. (Fig. 44)
9
Connect the power supply.
10
Start working.
13.4 Use of the Anti-glow cap
Use the anti-glow cap to prevent flare coming from the condenser
front lens. (Fig. 45)
13.5 Use of the UV shield
In order to
prevent possible damages from UV radiation, mount the
orange protection screen as shown. (Fig. 46)
2
14. Simultaneous observation in Phase Co Fluorescence
Fluorescence models allow
observation in transmitted light Phase contrast in combination
with reflected light Fluorescence. Samples with rapid decay must first be observed in
Fluorescence and then in Phase Contrast. The combined observation allows you to easily
identify some areas of the sample that emit fluorescence.
1
Turn on the microscope main switch.
2
Move the filter selector to an empty position or, if the filter holder is full, to the position containing
the UV filter.
3
Insert the desired PH objective and move the phase contrast slider to the position containing the
corresponding phase ring.
4
Focus the sample.
5
Adjust the light intensity of the transmitted light.
6
Move the fluorescence filter selector to the desired position.
7
Adjust the light intensity of the reflected light.
8
To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to
modulate the intensity of the fluorescence with the one of the phase contrast.
FIGURE 44
FIGURE 45
FIGURE 46
