VWR 630-3254, Instruction Manual

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IT406 FL4 / IT406 FL4D
13.3 Installing fluorescence filter
Only IT406 FL4 / IT406 FL4D
This procedure can be needed when installing new fluorescence
cubes or when replacing an existing cube with a different one.
1 Disconnect the power supply plug from the microscope.
2 Unscrew the filter selector lever.
3 Open the side cover of the illuminator, by unscrewing the side
screws (1). (Fig. 42)
4 Remove the filter slider from the microscope and put it on a table.
5 Using the screws provided with the fluorescence cube, fix the new
cube on the slider. (Fig. 43)
1
2
FIGURE 42
FIGURE 43
Filter name Excitation filter Dichroic mirror Barrier filter Applications
VisiCube IT_B 325-375 nm 415 nm 435LP nm
– DAPI
– Hoechst
VisiCube IT_G 390-420 nm 440 nm 450LP nm
– Coumarin
– Pacific Blue
VisiCube IT_V 455-495 nm 500 nm 510LP nm
– FITC
– GFP
VisiCube IT_UV 510-550 nm 570 nm 575LP nm
– Rhodamine
– TRITC
– Propidium Iodide
VisiCube IT_R1 582-603 nm 610 nm 615-645 nm
– Red#2 FISH
VisiCube IT_R2 590-650 nm 660 nm 665LP nm
– Cy5
VisiCube IT_R3 595-645 nm 655 nm 665-715 nm
– Cy5
– AlexaFluor 647
– Draq5
VisiCube IT_FR 623-678 nm 685 nm 690-750 nm
– Cy5.5
VisiCube IT_A 720-760 nm 770 nm 780LP nm
– Li_Cor IR dye 800