After the unit is powered back on with the IR sensor reinstalled, the display
toggles between “CYC COMPLETE”, “REPL HEPAs”, and “PRESS
ENTER”.
10. Open the chamber and install a new HEPA filter, air sample filter and
access port filter assembly. Press ENTER.
11. When ENTER is pressed, the LED goes out, the display returns to
“SYSTEM OK”, and the HEPA filter replacement timer is reset.
12. Fill the humidity pan with 3 liters of sterile distilled water with the
recommended purity range (See Section 1).
13. Allow to stabilize for at least 12 hours at the required temperature and
CO
2
level. It is recommended that the temperature and CO
2
calibrations be verified periodically during the first week of returning
the unit to service.
Caution
The high temperature sterilization cycle may cause the T/C CO
2
sensor output to change significantly. (This is normal and does not indicate
damage to the sensor.) Therefore it is essential that the CO
2
calibration is
verified before returning the unit to service.
s
If an independent instrument is not available to verify calibration, the
following procedure may be performed. After the Sterilization Cycle and
before CO
2
is allowed to enter the cabinet;
1) Fill and install the humidity pan.
2) Allow to stabilize at desired operating temperature for at least 12 hours.
3) Calibrate the display to 0.0%.
Note
If the Cool Phase is canceled, the display toggles between “CYC
CANCELED” and “COOL PHASE”.
• With T/C CO
2
sensor - When the temperature cools down to the
original set operating temperature or 30°C, whichever is higher, the
display toggles between “CYC CANCELED”, “REPL HEPAs”, and
“PRESS ENTER”. The white LED is lighted but no longer flashes.
• With IR CO
2
sensor - When the temperature cools down to the
original set operating temperature or 30°C, whichever is higher, the
display toggles between “CYC CANCELED”, “POWER OFF”, and
“REPLACE IR”. The white LED is lighted but no longer flashes. After
the unit is powered back on with the IR sensor reinstalled, the display
toggles between “CYC CANCELED”, “REPL HEPAs” and “PRESS
ENTER”.
Sterilization Cycle
(continued)
Air Jacketed CO2 Incubator
5-11
VWR International
Section 5
Routine Maintenance
