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VWR 10810-902, Operating Instructions Manual

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VWR 10810-902 Operating Instructions Manual Download Page 4

VWR International

Air Jacketed CO2 Incubator

iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.

4

Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4

Always dissipate extreme cold or heat and wear protective clothing.

4

Always follow good hygiene practices.

4

Each individual is responsible for his or her own safety.

Summary of Contents for 10810-902

Page 1: ...Operating Instructions VWR Air Jacketed CO2 Incubator ...

Page 2: ...ON By VWR Catalogue Number Manufacturer Model Number Sensor Capacity cu ft Voltage 10810 902 VWR51014991 T C 6 5 115V 60Hz 10810 944 VWR51014992 IR 6 5 115V 60Hz 10811 002 VWR50114902 T C 6 5 X 2 115V 60Hz 10811 004 VWR50114903 IR 6 5 x 2 115V 60Hz Legal Address of Manufacturer United States VWR International LLC 100 Matsonford Rd Radnor PA 19087 800 932 5000 http www vwr com Country of origin Uni...

Page 3: ...tain an optimal environment for the incubation of tissue and cell samples These models are designed to maintain temperature and carbon dioxide levels as set by the operator as well as monitoring chamber relative humidity CO2 is controlled using either a thermo conductivity cell or an infra red sensor The VWR Air Jacketed Incubators are approved for general purpose use only Non intended Use The VWR...

Page 4: ...ent being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures 4 Always use the proper protective equipment clothing gloves goggles etc 4 Always dissipate extreme cold or heat and wear protective clothing 4 Always follow good hygiene practices...

Page 5: ...ontact your local VWR representative or visit vwr com VWR can supply technical information about proper setup operation or troubleshooting of your equipment We can fill your needs for replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Maintenance Program for your products Whatever VWR products you need or use we will be happy to discuss y...

Page 6: ...he Humidity Pan 1 11 Connecting the CO2 Gas Supply 1 12 Incubator Start Up 1 13 Calibration 2 1 Temperature 2 1 Thermal Conductivity CO2 System 2 2 Infrared CO2 System 2 3 Configuration 3 1 Turn Audible Alarm ON OFF 3 1 New HEPA Filter 3 1 Set REPLACE HEPA filter reminder 3 2 Set Access Code 3 2 Enable Low Temp Alarm to Trip Contacts 3 3 Set Low Temp Alarm Limit tracking alarm 3 3 Set High CO2 Ala...

Page 7: ...tenance 5 1 Clean Cabinet Exterior 5 2 Replace Power Fuses 5 3 HEPA Filter Maintenance 5 3 Clean Glass Doors 5 3 Clean Humidity Pan 5 3 Replace Air Sample Filter 5 4 Electronics Section 5 4 Sterilization Cycle 5 6 Factory Options 6 1 Remote Alarms 6 1 Specifications 7 1 Customer Installed Accessories and Parts 8 1 Exploded Part Drawing 8 2 Warranty Information 9 1 Table of Contents Section 4 Secti...

Page 8: ...ains Disconnect Control Panel Keypad displays indicators Figure 1 2 Leveling Legs Used to level the unit Sterilization Cycle Button Switch to initiate sterilization cycle Note The incubators are stackable Information follows s Figure 1 1 Components Sterilization Cycle button Control panel Door heater cable See Section 5 See Section 1 Main power switch Chamber gas sample port Inner door Leveling le...

Page 9: ...Run Menu SET Set Points Menu CAL Calibration Menu CON Configuration Menu HEAT Indicator Lights when heaters are activated Injection INJ Indicator Lights during CO2 injection into chamber Temp T C Display Programmable to display temperature See Configuration section CO2 CO2 Display Programmable to display CO2 percentage See Configuration section RUN SET CAL CON MODE HEAT INJ T C CO 2 SCROLL FOR PAR...

Page 10: ...ys Steps the operator through parameters of SET CAL and CON modes The right arrow goes to the next parameter the left arrow returns to the previous parameter Up Down Keys Increases decreases or toggles the parameter value select ed in SET CAL and CON modes ENTER Must press this key to save to memory all changed values Keypad Operation RUN SETPOINT CALIBRATION CONFIGURATION Default Mode Temperature...

Page 11: ...ation space is needed on each side 2 Locate the unit on a firm level surface capable of supporting the unit weight of 260 lbs 118kg 3 Locate the unit away from doors and windows and heating and air conditioning ducts 4 Lift the unit by the sides of the cabinet base Do not attempt to lift it by the front and back This places stress on the outer door hinges Warning Install stacked units against a wa...

Page 12: ...hen lifting Lift the unit by the sides of the cabinet base to avoid placing stress on the outer door hinge s 5 Insert the stacking brackets into the slots at the back of the stacked units as shown in Figure 1 7 6 Align the slotted holes in the brackets with the mounting holes on the back of the top incubator Secure the brackets with the screws and washers provided in the parts bag See Figure 1 8 A...

Page 13: ...rer users should check with the manufacturer that the proposed method will not damage the equipment Accidental spills of hazardous materials on or inside this unit are the responsibility of the user s 1 Installed included grommets into the back flange of each duct sheet 6 grommets per sheet See Figure 1 10 2 Install the side ducts with the tabs facing into the center of the chamber with their slot...

Page 14: ...posite side It should snap into place See Figures 1 13 and 1 14 7 Slide end cap over spring Figure 1 15 Note When installing the rods the spring end can be installed on either side of the chamber The upper front rod is high in the chamber and the upper back rod is lower to allow access to the access port filter door Air Jacketed CO2 Incubator 1 7 VWR International Section 1 Installation and Start ...

Page 15: ...left corner of the interior chamber Remove the tape from the opening on the outside of the unit 2 Locate the stopper with filter in the hardware bag Lift the metal port cover and install the assembly in the opening inside the chamber See Figure 1 17 3 Also in the hardware bag is the CO2 sensor cover plate Install using the 1 4 turn fasteners Refer to Figure 1 17 for the location Installing Duct Sh...

Page 16: ...oid damage to the incubator do not operate the unit without the HEPA filter in place s Check the unit for being level by placing a bubble style level on one of the shelves Turn the hex nut on the leveler counterclockwise to lengthen the leg or clockwise to shorten it Level the unit front to back and left to right See the serial tag on the side of the unit for electrical specifications or refer to ...

Page 17: ...cm or a conductivity range of 20 0 to 1 0 uS cm Refer to ASTM Standard D5391 93 or D4195 88 for measuring water purity Distillation systems as well as some types of reverse osmosis water purity systems can produce water in the quality range specified Tap water is not recommended as it may contain chlorine which can deteriorate the stainless steel Tap water may also have a high mineral content whic...

Page 18: ... areas where concentrations of carbon dioxide gas can accumulate s Warning This incubator is designed to be operated with CO2 gas only Connecting a flammable or toxic gas can result in a hazardous condition Gases other than CO2 should not be connected to this equipment CO2 gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve Check the gas cylinder for the ...

Page 19: ... is labeled CO2 Inlet 1 Tank Make sure that the connections are secured with clamps Check all fittings for leaks With the incubator properly installed and connected to power the humidity pan filled and the unit connected to gas supplies system setpoints can be entered The following setpoints can be entered in SET mode Temperature Overtemperature and CO2 To enter SET mode press the MODE key until t...

Page 20: ...rol failure Temperature control in the incubator will be 1 of the overtemp setpoint The overtemperature is set by the factory default at 40 C However the overtemp can be set up to 55 C in 0 5 increments If the incubator s operating temperature setpoint is set above the overtemp setpoint the overtemp setpoint will automatically update to 1 C above the temperature setpoint It is recommended that the...

Page 21: ...nt range of 0 0 to 20 0 The incubator is shipped from the factory with a CO2 setpoint of 0 0 At this setting all CO2 control and alarms are turned off To change the CO2 setpoint 1 Press the MODE key until the SET indicator lights 2 Press the right arrow until CO2 XX X is displayed in the message display 3 Press the up down arrows until the desired CO2 setpoint is displayed 4 Press ENTER to save th...

Page 22: ...rease Numbers increase Numbers increase Press MODE to move to CALIBRATE mode Numbers decrease Numbers decrease Numbers decrease Press Enter to save setting Press Enter to save setting Press Enter to save setting Mode OTEMP XX X C Press to return to previous parameter Press to return to previous parameter Press to return to previous parameter Percent CO2 Over Temperature Operating Temperature To Se...

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Page 24: ... should be aware of the following system functions While the unit is in Calibration mode all system control functions are stopped so the unit remains stable Readout of the system being calibrated appears on the message display If no keys are pressed for approximately five minutes while in Calibration mode the system resets to RUN mode so control functions are reactivated Caution Before making any ...

Page 25: ... so any change in thermal conductivity is caused by a change in CO2 concentration Changing temperature or changing from elevated humidity to room ambient humidity levels will necessitate a re calibration of the CO2 control Some T C CO2 sensors go through an aging period especially on new installations Calibration should be checked on a weekly basis and adjusted as necessary When stabilization occu...

Page 26: ...left arrows to go to the next previous parameter Model VWR51014992 VWR catalogue number 10810 944 has an infrared IR CO2 sensor Infrared CO2 sensors are not effected by chamber atmosphere temperature or humidity However the light detector in the sensor is effected by wide temperature changes Therefore changing temperature setpoints could necessitate a recalibration of the CO2 Chamber temperature s...

Page 27: ...MODE key until the CAL indicator lights 3 Press the right arrow until IR CAL XX X appears in the message display 4 Press the up down arrow to adjust the display to match the independent instrument reading 5 Press ENTER to store the calibration 6 Press the MODE key to return to RUN mode 2 4 Air Jacketed CO2 Incubator VWR International Section 2 Calibration Infrared CO2 System continued ...

Page 28: ...rs decrease Numbers decrease Numbers decrease Press Enter to save the setting Mode CO2 ZR XX X Press to return to previous parameter Press to return to previous parameter Span CO2 Zero CO2 Operating Temperature Span CO2 when Thermal Conductivity sensor is in use when Thermal Conductivity sensor is in use when IR sensor is in use Chart 2 1 Calibration Mode Press Enter to save the setting Press Ente...

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Page 30: ...CON indicator lights 2 Press the right arrow until AUDIBLE XXX shows in the message display 3 Press the up down arrow to toggle AUDIBLE ON OFF 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter When the REPLACE HEPA reminder displays and the visual alarm flashes the specified time has elapsed and the HEPA filter should ...

Page 31: ... previous parameter Note After the reminder has been set check the allotted time remaining by going to Configuration mode then pressing the right arrow until NEW HEPA XXX displays This number is the remaining days before the filter replacement time specified runs out For example if 12 months was chosen in the REPL HEPA XX message display the NEW HEPA number would be 365 days s A 3 digit Access Cod...

Page 32: ... low temp alarm limit 4 Press ENTER to save the low temp alarm limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter The low temperature alarm can be programmed to trip the remote alarm contacts A setting of ON allows this an OFF setting blocks the low temp alarm from tripping the contacts The factory setting is ON 1 Press the MODE key until the CON ...

Page 33: ...to RUN mode or right left arrow to go to next previous parameter The high CO2 alarm limit is the deviation from the CO2 setpoint which will cause a high CO2 alarm The setpoint is variable from 0 5 CO2 above setpoint to 5 0 CO2 above setpoint The factory setting is 1 0 CO2 above setpoint 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until CO2 HI X X shows in the messag...

Page 34: ...being installed the two numbers on the factory installed sticker on the T C cell must be entered to calibrate CO2 in the unit Note For the technician s convenience a label containing the two numbers on the T C cell is affixed inside the electronics drawer s 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until T CZ XXXX shows in the message display 3 Press up down arrow...

Page 35: ...ide the electronics drawer s 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until T CS XXXX shows in the message display 3 Press up down arrow to change the span number to match the sticker 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter 3 6 Air Jacketed CO2 Incubator VWR International Se...

Page 36: ... Mode Audible alarm ON Press MODE to return to RUN mode Audible alarm OFF Press Enter to save the setting AUDIBLE XXX Audible Alarm Mode Press Enter to restart the timer and clear the REPLACE HEPA alarm Press to return to previous parameter Numbers increase Numbers decrease Chart 3 1 Configuration Mode Page 1 of 2 Press MODE to light CON To Configure Mode Press Enter to save the chosen of months N...

Page 37: ...ss to return to previous parameter Press to return to previous parameter Press to return to previous parameter refer to previous page Chart 3 1 Configuration Mode Page 2 of 2 T C Zero Number CO2 Low Limit CO2 High Limit T C Span Number CO2 HI X X CO2 RLY XXX Numbers decrease Press Enter to save the setting Numbers increase Press Enter to save the setting Mode Press to return to previous parameter ...

Page 38: ...to ring back in 15 minutes The TEMP IS LOW alarm is disabled when the Temp set point is 10 C The CO2 alarms are disabled when the CO2 set point is 0 0 Description Message Delay Ringback Relay No alarm condition exists SYSTEM OK or CLASS 100 Temp Otemp Set point SYS IN OTEMP 0 min 15 min Yes Air Temp Sensor Fault See Section 4 AIR SNSR ERR 0 min 15 min No Temperature Controller Failure See Sect 4 T...

Page 39: ...60 C 5 This is intended to be a safety feature to protect the incubator and is not intended to protect the cell cultures or the equipment inside the chamber should a temperature control failure occur Should such a failure occur contact the Technical Services Department or your local distributor The microprocessor in VWR Air Jacketed Incubators continually scans all available sensors to ensure that...

Page 40: ... of preventive maintenance requirements See the specified section of the instruction manual for further details We have qualified service technicians using NIST traceable instruments available in many areas For more information on Preventive Maintenance or Extended Warranties please contact 5FDIOJDBM 4FSWJDFT Cleaning and calibration adjustment intervals are dependent upon use environmental condit...

Page 41: ...ter level in the humidity pan from top 2 Verify and document CO 2 humidity and temperature calibration as applicable See Calibration Disinfect the interior of the incubator See Routine Maintenance 1 Replace HEPA access port filter air sample filter and CO 2 filters if applicable or as needed 5 Perform sterilization cycle as needed Preventive Maintenance ...

Page 42: ...ol even a 70 solution is volatile and flammable Use it only in a well ventilated area that is free from open flame If any component is cleaned with alcohol do not expose the component to open flame or other possible hazard Do not spray the T C sensor with flammable solutions The internal temperature of the CO2 sensor is approximately 150 C when the unit is in operation Allow sufficient time for th...

Page 43: ...working down Refer to the disinfectant directions for length of time needed before rinsing Wash the inner door both inside and out The cabinet and door must be rinsed with sterile water until the disinfectant has been removed After the cabinet has been rinsed spray with 70 alcohol 5 Install the left and right ducts inner door gasket access port filter assembly and air sample filter and tubing spra...

Page 44: ...general use laboratory disinfectant Rinse with sterile water and spray with 70 alcohol The pan may be autoclaved Replace the HEPA filter when the REPLACE HEPA reminder is displayed The REPLACE HEPA reminder can be set to alarm after a specified time from 1 to 12 months The reminder default is the factory recommended setting of 6 months For details see Section 3 To access the only replaceable fuse ...

Page 45: ... ceiling 2 Connect the other end of the filter to the metal tubing on the HEPA filter adapter Figure 5 2 Figure 5 2 Connect to Metal Tubing 5 4 Air Jacketed CO2 Incubator VWR International Section 5 Routine Maintenance Metal tubing Air Sample Filter Replace Air Sample Filter ...

Page 46: ...nterior baked on material etc wipe off all visible signs of spills Odor may occur during the Sterilization Cycle and is considered normal The Steri Cycle is not intended to sterilize other items instruments etc from the lab During the cycle the unit chamber becomes hot enough to melt samples instruments dishes etc left inside the unit The items listed below also need to be removed 1 HEPA filter 2 ...

Page 47: ...y I R sensor is not installed The sensor cable cannot withstand sterilization cycle temperatures and is destroyed properly The outer door is opened during the heat or sterilization phases An outer door alarm occurs CLOSE DOOR in the display plus an audible cannot be silenced and visual alarm The outer door is open longer than 20 seconds during phases listed above Cycle is canceled unit goes to CAN...

Page 48: ...inute the display returns to SYSTEM OK 5 Remove the HEPA filter air sample filter and access port filter assembly Figure 5 5 Tubing can remain in the unit during the sterilization cycle In addition remove the temp RH recorder probe and IR sensor if applicable Discard the filters 6 Pre Sterilization with IR CO2 sensor If an IR sensor is connected the display toggles between POWER OFF and REMOVE IR ...

Page 49: ...ensor disinfection procedure Connect the dummy sensor to the cable Fit the installed dummy sensor up into the previous sensor location When the power is turned on with the IR sensor removed the display toggles between REMOVE HEPAs REMOVE WATER and PRESS ENTER If ENTER is not pressed within 1 minute the display toggles between POWER OFF and REPLACE IR When the unit is powered back on with the IR se...

Page 50: ...he air temperature in the unit reaches 140 0 C the Sterilization Phase begins and the display changes to STERILIZING 8 After approximately 2 hours a 5 second audible tone sounds signaling STERILIZING HEAT PHASE Figure 5 15 Heat Phase STERILIZING 140 Figure 5 16 Sterilization Phase STERILIZING COOL PHASE Figure 5 17 Cool Phase Sterilization Cycle continued Air Jacketed CO2 Incubator 5 9 VWR Interna...

Page 51: ...ture or 30 C whichever is higher the display toggles between CYC COMPLETE POWER OFF and REPLACE IR The white LED is lighted but no longer flashes Clean the IR sensor with isopropanol Simply spray the sensor do not saturate and allow to dry Do not saturate the sensor or immerse the sensor in a cleaner Power the unit off open the chamber and remove the IR plate Pull down on the dummy unscrew the cab...

Page 52: ...the unit to service s If an independent instrument is not available to verify calibration the following procedure may be performed After the Sterilization Cycle and before CO2 is allowed to enter the cabinet 1 Fill and install the humidity pan 2 Allow to stabilize at desired operating temperature for at least 12 hours 3 Calibrate the display to 0 0 Note If the Cool Phase is canceled the display to...

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Page 54: ...mperature condition The contacts may also be programmed to trip or not trip on temperature alarms CO2 alarms and RH alarms See Section 3 Configuration mode Air Jacketed CO2 Incubator 6 1 VWR International Remote Alarms COM yellow wire NO green wire phone cable to incubator alarm contacts RJ 11 connector Alarm Contacts Junction Box All outputs shown in alarm condition NC red wire not connected blac...

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Page 56: ...mmable action and indicator Display Digital LED 0 1 C increments Temperature Safety Type Extreme temperature safety action and indicator Sensor Thermostat independent of temp control system Indicator Message center audible and visual alarms Relative Humidity Control Humidity pan natural vaporization Humidity w pan 95 RH at 37 C CO2 Control 0 1 microprocessor PID control Sensor T C or IR Readabilit...

Page 57: ...uge cold roll steel Outer door gasket Four sided molded magnetic Santoprene Inner door gasket Bulb silicone Insulation Mineral wool Fittings Access port 1 1 4 32mm removable silicone plug CO2 inlet 1 4 6 4mm barbed Sample port Front mounted barbed Electrical 115VAC 50 60 Hz 1PH 9 6 FLA Operating range 90 125VAC Power switch circuit breaker 2 Pole 12 0 Amp Accessory outlet Voltage equal to the cabi...

Page 58: ...lation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and ...

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Page 60: ...ssories and Parts VWR Part Number Description 98000 392 Stainless steel shelf and channels 10065 492 HEPA filter 5 5 diameter 97025 844 HEPA filter replacement kit 10820 346 Roller base Air Jacketed CO2 Incubator 8 1 VWR International ...

Page 61: ... damaged by accident abuse misuse or misapplication or from ordinary wear and tear Expendable items glass filters and gaskets are excluded from this warranty THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES WHETHER WRITTEN ORAL OR IMPLIED NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY Items being returned must be insured by the customer against possi...

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