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18

 

 

If the “No. of Replics” is “3”, then place the second solution of STD No.1 in the 
cuvette holder. Pressing 

ENTER

 will measure the second solution of STD N0.1 

and the second measurement value will be displayed at STD No.1= 0.18775A2. 
Repeat the same procedures to measure the third solution of STD No.1 and screen 
will display STD. No.1=0.1876A3. This finishes the measurement of the three 
solutions of standard No. 1. 

 

Press 

ENTER 

again to average the measured absorbance values and the screen 

then displays its averaged value: 0.1876A  (0.0.0.1876A3)/3. 
This averaged absorbance value will be used for calculation. 

 
8.5

 

     Place standard solution No.2 into the cuvette holder in the optical path. Repeat step 
     8.3 to 8.4 to measure standard No.2. Repeat the same procedures for all the              
     standards in sequence.  

 
8.6

 

   After all the standards are measured press ENTER to create the curve and the 
screen return to the first with 

 * at “Name Curve”.

 

 

 

 

9. 

Name Curve and Save Curve: Refer to “Name Curve and Save Curve” in “By 
Factor” chapter in this manual. 

10. 

Display and Print Curve Parameters: Refer to “Dis & Print Curve” in By Known 
Factors chapter in this manual. 

 

 

UTILITY 

 

 

 

 
In 

MAIN MENU

, move * down to the left of 

UTILITY 

and press 

ENTER

 to go into 

UTILITY

 

menu. 

Summary of Contents for S1205 SERIES

Page 1: ...S1205 SERIES SPECTROPHOTOMETER USER S MANUAL V 3 0 United Products Instruments Inc 182 E Ridge Road Dayton NJ 08810 732 274 1155 FAX 732 274 1151 TABLE OF CONTENTS...

Page 2: ...C MODE T Abs Measure 10 Quantitative Test 12 Run test 12 Define Establish Test Method 14 By Known Factors 15 By Standard 18 UTILITY 21 WL Calibration 22 Dark Current 22 EDIT CLOCK 22 SYSTEM BASELINE 2...

Page 3: ...the HEALTH AND SAFETY AT WORK ACT 1974 Read the following before installing and using the instrument and its accessories The S1205 should be operated by appropriate laboratory technicians Electrical...

Page 4: ...te Water Labs and other fields of quality control and research Both S1205 features a digital display of photometric result easy operation and wavelength range of 325nm to 1000nm the instrument is idea...

Page 5: ...B port for PC connection and RS 232 for printing Set the switch to the right for firmware update via RS 232 port the USB port will not work at this setting Important Note The RS 232 port can also be u...

Page 6: ...Place the instrument in a suitable location away from direct sunlight In order to have the best performance from your instrument keep it as far as possible from any strong magnetic or electrical field...

Page 7: ...r Enter Known Factor value Browse the items in the menu Operation Instruction Prepare the spectrophotometer Turn on the spectrophotometer by pressing the Power Switch IO Press EXIT to skip 15 minutes...

Page 8: ...s hanging on for a long time or the environment is not stable BASIC MODE T Abs Measure 1 At main menu scroll to BASIC MODE and then press ENTER to Select basic T Abs mode If the readouts are not 100 0...

Page 9: ...the cuvette is in the light path Close the lid 5 Set 0 000A or 100 T by pressing 0A 100 T button 6 Remove the blank cuvette if you are testing more than 3 samples Set it aside in the case that you ma...

Page 10: ...at steps 2 to 9 for each wavelength 11 For each new sample you analyze repeat steps 3 to 9 Quantitative Test Run Test using pre defined Method Test method curve must be defined and established before...

Page 11: ...test run If the desired test is defined and saved in All Test List but not yet loaded in Run Test you need to load the test method to Run Test before you can run the quantitative tests using such met...

Page 12: ...an t run any test from All Test List Only run test in Run Test menu Define Establish Test method The 200 un defined tests are originally named To be defined 001 to 200 You can choose to rename any of...

Page 13: ...er linear Concentration Intercept Slope Absorbance 1st order linear through zero Concentration Slope Absorbance Press Enter to confirm fitness method selection The screen returns to previous menu and...

Page 14: ...st order liner through 0 is selected there is no need to enter intercept 8 Name the Curve The name character entry is the same way as the entry of intercept and slope 8 1 To select a number or charact...

Page 15: ...er linear 0 represents 1st order linear through zero Intercept value 1 058 in thise case Slope number 2 588 in this case and Concentration unit ppm in this case Note N and M are not part of parameter...

Page 16: ...r to left The scroll cursor to right 5 2 Press to confirm and enter the number 5 3 Use CLEAR to delete erase any incorrect entry 5 4 Check and make sure the number is correct Then press ENTER to confi...

Page 17: ...owing screen will pop up 8 2 Place the cuvette with reference solution in the cuvette holder press 0Abs 100 T to blank After blank is complete it is ready for standard solution measurement starting fr...

Page 18: ...lue 0 1876A 0 1877A1 0 1875A2 0 1876A3 3 This averaged absorbance value will be used for calculation 8 5 Place standard solution No 2 into the cuvette holder in the optical path Repeat step 8 3 to 8 4...

Page 19: ...refresh instrument dark current It is designed for eliminating the error from electronic drifting It is recommended that you refresh and save the Dark Current after the 15 minutes warm up and periodi...

Page 20: ...clock is not running accurately Set the off set level clock trimming to adjust the clock There are 64 levels of adjustments Slow Levels Fast Levels Slowest Fastest 32 16 0 0 16 32 Cable 1 Select Cloc...

Page 21: ...display it on screen or you can send the data to computer B Clear User Tests Select Clear User Tests all established and saved tests curves will be deleted Warning All saved test curves will be delete...

Page 22: ...ss ENTER the following screen is displayed When the communication between the instrument and the computer is established the computer is in control Please details of the PC software please refer to UN...

Page 23: ...nce by pressing the 0A 100 T The reading should then be 0 000A If not press 0Abs 100 T again 4 Remove the cuvette holder and insert the Holmium filter into it Place it in the sample compartment and cl...

Page 24: ...hecked against a set of neutral density filters accurately calibrated to the NIST standards Contact your UNICO representative for more information 800 588 9776 An alternative method using potassium di...

Page 25: ...ngineer Improper power setting Set to the main power Instrument cannot set 100 T 0 000A Light beam blocked Check sample holder See if holder is properly positioned and nothing is blocking light path L...

Page 26: ...velength accuracy according to procedure in this manual Stray sample preparation vapors Prepare sample away from instrument Use proper ventilation Bubbles or particles in solution Check sample prepara...

Page 27: ...ng driven motor Check the connector J5 on CPU board Call authorized service engineer Error 4 Instrument unable to locate 0 order light Check sample holder See if holder is properly positioned and noth...

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