UGO BASILE 47880-001, Instruction Manual

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4 Operation 4 Operation
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4.5 Calibration
Librae Incapacitance Tester is factory calibrated by Ugo Basile technicians. It is possible to
override the original calibration if required by a customer quality policy (or other reasons).
For this purpose, Librae guides the user through a wizard calibration procedure. To run the
procedure, press the CALIBRATION icon on the Home Page Menu (Figure 4.1.1).
NOTE: Before running the calibration wizard procedure, ensure that NO weight is applied on the
food pads, AND a 100g calibrated weight is present. If necessary, Ugo Basile can supply a 100g
calibrated weight. Refer to paragraph 7.0 for ordering details.
Figure 4.5.1 shows the calibration steps procedure. Follow the instructions on the screen to
calibrate your Librae Incapacitance Tester.
Figure 4.5.1
DO NOT REMOVE the weight during the calibration procedure.
Follow the instructions from the calibration wizard.
After the execution of all steps, Librae Incapacitance Tester will be calibrated with the new
values.
NOTE: Calibration is not necessary if it is not required by customer quality policy. The
instrument is shipped fully calibrated.
NOTE: During the calibration procedure, Librae MUST be positioned on a stable workbench.
In addition, Librae must be horizontal, level, and stationery. Avoid calibration if this is not
possible..
NOTE: During calibration procedure, the values are stored ONLY after the confirmation of the
last step. Before that, it is possible to escape the procedure just by pressing CANCEL button and
NO DATA WILL BE STORED.
4.6 Set Autostart
Autostart is a unique Librae Incapacitance Tester feature. By selecting this operative mode,
Librae helps the user to perform the experiment in a consistent way, reducing variability and
user bias or intervention during measurement.
Before using this feature, it is important to set the parameters in order to take advantage of
autostart.
Figure 4.6.1 shows the Autostart settings menu.
1. Autostart Weight Threshold: this parameter sets the minimum weight the Librae MUST
consider for animal. The percentage value from 10% to 90% is referenced to the animal
weight (Refer to paragraph 4.3)
2. Autostart Stabilization Threshold: this parameter sets the percentage weight variation
referenced to the animal weight (Refer to paragraph 4.3). Percentage value from 1% to 20%.
3. Autostart Sustain Time: this parameter sets the acceptable time for satisfying the previous
two parameters. Numeric value from 200 msec to 5 sec.
Figure 4.6.1
To help the user understand how this feature works, an example is shown below.
Let’s assume the test has a mouse that weighs 50 g, the experiment duration is 15 seconds, and
autostart mode is selected.
1. Configure the SETUP menu (refer. to paragraph 4.3) as follows:
1.1 Animal Max Weight: 60g;
1.2 Measurement Duration: 15s;
1.3 Start Mode; Auto.
2. Press the SET AUTOSTART icon, and set the parameters as follows:
2.1 Autostart Weight Threshold: 60%. With this value, Librae considers the presence
of the animal ONLY IF MORE THAN 36g (60% of 60 grams) are measured.
2.2 Autostart Stabilization Threshold: 10%. With this value, Librae considers that the
animal is quiet and ready for experiment ONLY IF the NOISE on foot pads is
LESS THAN 6g (10% of 60 grams). See Figure 4.6.2 for details.
2.3 Autostart Sustain Time: 3s. With this value Librae NEEDS AT LEAST 3S in which the
previous conditions are satisfied.
3. Go to Home Page Menu, and press START button (Figure 4.6.2);