998-2821
Rev. D
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3) Once bubbles have been removed select the Blank Calibration button from the LD2
software screen. The dialog box will then state “Blank Calibration…Please wait”.
4) Once the blank calibration is complete the following screen will appear:
5) Remove the Little Dipper
2 from the beaker.
6) In the same beaker which contains a known volume of your source water, make a
solution using your fluorophore of interest that represents a concentration you intend to
measure or use Turner Designs prepared calibration standards. Contact
7)
Place the beaker on a non-reflective black
surface.
Ensure the solution is well mixed,
submerge the Little Dipper
2 into the solution.
IMPORTANT NOTE: MAKE SURE TO REMOVE ALL BUBBLES FROM THE OPTICAL
FACE BY TILTING THE LITTLE DIPPER
TM
2 AND GENTLY SHAKING WHILE FULLY
SUBMERGED IN THE WATER AS AIR BUBBLES TRAPPED NEAR OR ON THE
OPTICAL HEAD WILL INFLUENCE RESULTS.