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3. Begin LD2 software by double-clicking on the LD2 icon.
4. Plug the 12V power supply into a power source and supply power to the
calibration cable.
5.
Wait for initialization process to complete.
6. Select the Connect to Device button. The Device Connection Status will turn
green when connection is established
and “Device is now connected” will appear
in the dialog box.
3.2 Calibration
Once the connection to the Little Dipper
2 has been established in the LD2 Software you
can proceed with calibration.
1) Obtain a glass beaker with a known volume of the
source water you will be measuring.
Place the beaker on a non-reflective black surface.
Note: This water should represent the signal
you intend to set as zero, make sure that it
does not contain
any fluorophore.
2) Fully submerge the optical face in the water.
IMPORTANT NOTE: MAKE SURE TO REMOVE ALL BUBBLES FROM THE OPTICAL
FACE BY TILTING THE LITTLE DIPPER
TM
2 AND GENTLY SHAKING WHILE FULLY
SUBMERGED IN THE WATER AS AIR BUBBLES TRAPPED NEAR OR ON THE
OPTICAL HEAD WILL INFLUENCE RESULTS.
