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TriContinent 8441 series, Operation And Maintenance Manual

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TriContinent 8441 series Operation And Maintenance Manual Download Page 30

4.6

CLEANING MANIFOLD NEEDLES

A needle cleaning wire is supplied with each manifold, and may be used to unclog aspirate or 
dispense needles which have been plugged by particulates or dried salt deposits.  The 
standard manifold dispense needles are a composite assembly with an outside sheath of 
plastic, housing an inner precision bore glass capillary tube. Avoid bending the assembly when 
handling. The cleaning wire can be used when rinsing fails to clear a bore.

Multiwash III

25

Summary of Contents for 8441 series

Page 1: ...8618 01 E MULTIWASH III MICROPLATE WASHER OPERATION AND MAINTENANCE MANUAL ...

Page 2: ...t the following products Microplate Washer Model Numbers 8441 XY Where X 0 thru 9 Y 0 thru 9 to which this declaration relates is in conformance with the following standards EN61010 1 2001 EN61326 1 2006 EN61326 2 6 2006 Following the provisions of the 2006 95 EEC and 2004 108 EC Directives TriContinent has as our EU Authorized Representative Armin Foell TriContinent International Sales Manager lo...

Page 3: ... 2 DISPENSING 11 2 3 ASPIRATION 11 2 4 WASH CYCLE OPTIONS 12 2 5 KEYPAD 12 2 6 DISPLAY 13 2 7 AUTORINSE VALVE 13 3 OPERATION 14 3 1 OVERVIEW 14 3 1 1 STRIP WASH MODE 14 3 1 2 PLATE WASH MODE 14 3 1 3 PRIME 14 3 1 4 RINSE 15 3 1 5 ROWS 15 3 1 6 AUTOMATIC STANDBY ROW REFILL 15 3 1 7 WASTE RESERVOIR FULL SENSOR 15 3 2 OPERATING INSTRUCTIONS SUMMARY 16 3 3 PROGRAMMING A WASH CYCLE 17 3 3 1 PROGRAM NUM...

Page 4: ...TALLING MANIFOLD TUBING CONTINUED 28 4 9 FUSE MODULE REMOVAL AND FUSE INSTALLATION INSTRUCTIONS 29 4 10 DECONTAMINATION PROCEDURE 30 5 TROUBLESHOOTING 31 5 1 RE INITIALIZING THE STORED PROGRAMS 31 5 2 TROUBLESHOOTING TABLE 31 5 3 ERROR CODES 34 5 4 USER TROUBLE SHOOTING NOTES 35 6 SPECIFICATIONS 36 6 1 PERFORMANCE 36 6 2 MECHANICAL ELECTRICAL 36 APPENDIX 1 ACCESSORIES AND REPLACEMENTS 37 APPENDIX ...

Page 5: ...Multiwash III THIS PAGE INTENTIONALLY LEFT BLANK vi ...

Page 6: ...II instrument Reservoir kit Wash Rinse and Waste AC Power Supply Cables USA domestic 120V European 220V Bag containing spare fuses instructions and soaking microstrip wells This manual MultiWash III Operation and Maintenance Quick Start Guide 1 2 ENVIRONMENTAL REQUIREMENTS Locate the MultiWash III so that it is shielded from excess exposure to dust vibration strong magnetic fields direct sunlight ...

Page 7: ...area is shown in the correct manner as illustrated If you are not sure of the setting that is right for your area contact your local power company 1 3 1 120 LINE VOLTAGE SELECTION The small arrow below the selected 110 120V voltages shown on the Fuse Module must be aligned with the WHITE arrow on the Power Entry Module for proper OPERATION WARNING Disconnect power cord before continuing onto the n...

Page 8: ...aligned with the WHITE arrow on the Power Entry Module for proper operation WARNING Disconnect power cord before continuing onto the next steps Multiwash III 3 ILLUSTRATION OF THE PROPER ORIENTATION OF THE FUSE MODULE FOR OPERATION IN THE VOLTAGE RANGE OF 198 TO 264 VOLTS 220 VOLT POWER SUPPLY CABLE STEP 1 STEP 2 STEP 3 ...

Page 9: ...sher tubing Connect the white dispense fittings together and the clear aspirate fittings together Tubing should look like the illustration when it is correctly installed If the manifold tubing is to be replaced see section 4 8 to properly reposition it Place the soaking microstrip well into the slot as shown below Multiwash III SINGLE MICROSTRIP WELL USED WITH 8 AND 12 PORT STYLE MANIFOLDS CLEAR F...

Page 10: ... 1 5 1 THUMBSCREW ADJUSTMENT Adjust the thumb screw to ensure the manifold is level See the Figures below Multiwash III THUMB SCREW LEVEL 8 Port Standard Manifold Example THUMB SCREW LEVEL 12 Port Coaxial Manifold Example 5 ...

Page 11: ...tandard flat bottom microplate configuration This adjustment will also change the position of the manifold for the round or V bottom style microplate Plate Selection mode see Section 3 3 8 for programming information 4 Press and the manifold will rise and move to the factory defined adjustment row as shown in the examples The display will show the number 820 This number represents the factory defa...

Page 12: ...8 PORT STANDARD AND 12 PORT STANDARD MANIFOLDS POSITION SHOWN FOR FLAT PLATE SELECTION 8 PORT COAXIAL AND 12 PORT COAXIAL MANIFOLDS POSITION SHOWN FOR FLAT PLATE SELECTION Multiwash III 7 ...

Page 13: ... page The display will show 335 indicating the factory default position 4 Press to move the manifold up or to move it down Each press of the button will produce approximately 002 05mm movement of the manifold up or down The display will add or subtract 1 count for each press of a button Example If the aspirate needles are determined to be too deep in the well press while observing needle positioni...

Page 14: ... FINAL ASPIRATION WELL POSITION TOP OF WELL ASPIRATION POSITION Multiwash III 9 ...

Page 15: ... GREEN RECEPTACLE IN CAP OF WASTE BOTTLE INSTALL THE GREEN FITTING ON TUBING COMING FROM THE CONDUIT ON REAR PANEL TO OTHER GREEN RECEPTACLE IN CAP OF WASTE BOTTLE INSTALL THE WASTE LEVEL SENSOR FROM THE WASTE BOTTLE TO THE JACK AT TOP OF REAR PANEL INSTALL THE BLUE ELBOW FITTING ON THE END OF THE WASH TUBING INTO THE BLUE RECEPTACLE ON CAP OF WASH BOTTLE THEN INSTALL BLUE FITTING AT OTHER END OF ...

Page 16: ...nse sequence executes as follows A valve on the syringe assembly opens the port to the Wash solution reservoir and the pump moves in the fill direction to draw solution into the syringe When the syringe is full the valve moves to the dispense position The pump dispenses the volume of wash solution into the microplate wells called for by the wash program in use During the wash cycle the valve and s...

Page 17: ... liquid in that row of microplate wells The instrument then dispenses wash solution into row 1 and moves to the next row leaving wash solution in row 1 to soak This sequence is repeated for each row until the number of ROWS selected has been processed The instrument then returns the manifold to the soaking microstrip position until the SOAK SECONDS for the first row has elapsed The aspirate and di...

Page 18: ...the instrument will then begin a wash cycle when the Start Stop key is pushed The display will show error codes when there is a problem with the instrument and stop working Your distributor should be contacted for additional assistance see Appendix 2 and the troubleshooting guide in Section 5 2 and the error code table in Section 5 3 gives additional assistance 2 7 AUTORINSE VALVE During the Wash ...

Page 19: ...y row until the SOAK SECONDS for the first row has elapsed The aspirate and dispense is then repeated across the microplate completing the first wash CYCLE This sequence of passes across the microplate is repeated for the number of CYCLES selected At the end of the last CYCLE wash solution is aspirated from the wells All rows are soaked for the same length of time Each row is left with wash soluti...

Page 20: ...forms a maintenance rinse of the standby row after eight hours of non use It is important to leave the power on to the instrument at all times for this maintenance rinse function to occur Leaving the power on is critical if the Wash solution is not routinely purged from the instrument after use This rinse operation is designed to keep Wash solution from drying and leaving clogging salt deposits be...

Page 21: ...s Start Stop Press Start to begin processing the plate Press Stop to cancel the wash Press Stop again to return to the manifold to the soaking microstrip well The Prime key is used to prime the syringe pump lines and manifold with wash solution The Rinse key is used to rinse clean the syringe pump lines and manifold when the rinse tubing is connected to distilled water Step 2 Select of Rows Press ...

Page 22: ...ust the dispense volume by pressing or in 10 µl increments The display shows the per well volume regardless of 8 12 or 2 8 port manifold selection Maximum volume is dependent on the manifold selected see Section 6 1 for additional information 3 3 3 1 TO ASPIRATE ONLY A Set the DISPENSE VOLUME to zero B The number of WASH CYCLES must be adjusted to greater than zero The washer will then aspirate th...

Page 23: ...e dispensed into each well without aspiration 3 3 7 STRIP PLATE MODE WASH Press until the flashing arrow appears next to STRIP PLATE MODE WASH Press or to toggle between SP STRIP or PL PLATE wash modes When PL PLATE WASH MODE is selected the arrow will remain displayed as a visual reminder that Plate Wash mode has been selected for this program Select Review Select Review Select Review Select Revi...

Page 24: ...r this program 3 3 9 ROWS Press to display the number of rows selected Set the number of rows 1 up to 12 8 port manifold 1 up to 8 12 port manifold or 2 up to 12 in increments of 2 2 8 port manifold by pressing or Press and the display will show PROGRAM NUMBER again 3 3 10 START STOP Press to start the current wash program Press again to cancel the plate processing and stop the manifold at the las...

Page 25: ...nds Manifold Selection Wash Cycles Program Used for Dispense Volume Wash Mode Dispense Speed Plate Selection Soak Seconds Manifold Selection Wash Cycles Program Used for Dispense Volume Wash Mode Dispense Speed Plate Selection Soak Seconds Manifold Selection Wash Cycles Program Used for Dispense Volume Wash Mode Dispense Speed Plate Selection Soak Seconds Manifold Selection Wash Cycles Multiwash I...

Page 26: ...ds Manifold Selection Wash Cycles Program Used for Dispense Volume Wash Mode Dispense Speed Plate Selection Soak Seconds Manifold Selection Wash Cycles Program Used for Dispense Volume Wash Mode Dispense Speed Plate Selection Soak Seconds Manifold Selection Wash Cycles Program Used for Dispense Volume Wash Mode Dispense Speed Plate Selection Soak Seconds Manifold Selection Wash Cycles Multiwash II...

Page 27: ...RS It is recommended that the inlet line filters in both the Wash and Rinse reservoirs be back flushed at least once every week if wash solution is left standing in the bottle for long periods of time This removes trapped particles and prevents excessive back pressure on the dispense pump and syringe assembly The filters can be removed from the inlet tubings and a syringe filled with an appropriat...

Page 28: ...OF DAY FILL WASH RESERVOIR PRIME TO REMOVE BUBBLES CHECK DISP ASP PROBES CLEAN PROBES IF NEEDED END OF DAY FILL RINSE RESERVOIR RINSE CYCLE TO FLUSH LINES ADD 10 BLEACH TO WASTE EMPTY EMPTY WASH RESERVOIR WEEKLY BACKFLUSH RESERVOIR FILTERS MONTHLY DECONTAMINATE WITH BLEACH REPLACE EXHAUST FILTER Call TriContinent if equipment malfunctions Multiwash III 23 ...

Page 29: ... 27 28 29 30 31 START OF DAY FILL WASH RESERVOIR PRIME TO REMOVE BUBBLES CHECK DISP ASP PROBES CLEAN PROBES IF NEEDED END OF DAY FILL RINSE RESERVOIR RINSE CYCLE TO FLUSH LINES ADD 10 BLEACH TO WASTE EMPTY EMPTY WASH RESERVOIR WEEKLY BACKFLUSH RESERVOIR FILTERS MONTHLY DECONTAMINATE WITH BLEACH REPLACE EXHAUST FILTER Call TriContinent if equipment malfunctions Multiwash III 24 ...

Page 30: ...s which have been plugged by particulates or dried salt deposits The standard manifold dispense needles are a composite assembly with an outside sheath of plastic housing an inner precision bore glass capillary tube Avoid bending the assembly when handling The cleaning wire can be used when rinsing fails to clear a bore Multiwash III 25 ...

Page 31: ...dispense needles Insert the needle service tool under the dispense needle collar Pry the dispense needle upward with the tool This will loosen the needle from its press fit with the manifold Place the new needle into the open end of the needle service tool and firmly insert it into the manifold Care should be exercised to prevent breakage of the precision glass insert Multiwash III 26 ...

Page 32: ...IFOLD ASSEMBLY A AND TUBING SYRINGE MANIFOLD ASSEMBLY B AS SHOWN ABOVE THREAD THE CLEAR FITTING ON TUBING ASSEMBLY A THRU THE CONDUIT FROM THE REAR OF THE UNIT AS SHOWN THREAD THE CLEAR FITTING ON TUBING ASSEMBLY B THRU THE CONDUIT FROM THE FRONT OF THE UNIT AS SHOWN 2 CONDUIT CLEAR FITTING THREAD THRU FROM FRONT WASTE MANIFOLD TUBING ASSEMBLY A CLEAR FITTING THREAD THRU FROM BACK 2 SYRINGE MANIFO...

Page 33: ...EASURE TO OF TUBING FROM THE FRONT OF THE CASE TO THE END OF THE LUER FITTING AS SHOWN ABOVE INSTALL THE BLACK CAP TO CLOSE OFF THE FRONT END OF THE CONDUIT THREAD BOTH LUER FITTINGS THROUGH THE TUBING CLIP 1 2 3 4 SLOT IN CONDUIT ON RIGHT SIDE 1 INSTALL BLACK CAP OVER CONDUIT 3 WHITE FITTING CLEAR FITTING CLEAR FITTING REF WHITE FITTING REF 10 266 mm APPROX 2 4 ...

Page 34: ...HE SLOT AT THE TOP MIDDLE OF FUSE DRAWER AS SHOWN TEMPORARILY PLACE FUSE DRAWER ON BENCH 1 2 LOOKING AT FRONT RIGHT SIDE OF FUSE DRAWER WITH 110 120V WORDING AT THE BOTTOM 3 INSTALL A AMP FUSE INTO FUSE CLIPS AT RIGHT SIDE OF FUSE DRAWER AS SHOWN 3 5 RE INSTALL FUSE DRAWER AS REQUIRED FOR THE SUPPLIED POWER PER SECTION 1 3 FRONT VIEW OF POWER MODULE WITH FUSE DRAWER RE INSTALLED 4 LOOKING AT FRONT...

Page 35: ...is procedure does not guarantee total decontamination of the Multiwash III 8 Wear disposable gloves at all times while servicing CAUTION RESERVOIR BOTTLES CANNOT BE CLEANED IN A DISHWASHER Before returning any items call TriContinent see Appendix 2 to obtain a Return Material Authorization RMA number If the instrument has been exposed to hazardous substances the instrument MUST be cleaned and deco...

Page 36: ...ect position See Section 1 3 2 Instrument doesn t respond to key presses Invalid data in instrument s stored programs Caused by voltage spikes or fluctuations Hold Down Arrow key while turning on power to restore operation A line conditioner or surge protector needs to be used See Section 1 2 3 Manifold hits plate when aspirating rows The manifold is not calibrated to the proper position within th...

Page 37: ...Waste reservoir is loose Tighten cap Manifold needles are not parallel to the well strips Adjust the manifold so that it is level See Section 1 5 1 7 Waste reservoir sensor does not register FUL when submerged in liquid Accumulated residue preventing float switch from functioning Clean float switch on probe attached to inside of waste bottle cap 8 Manifold hitting plate after washing several rows ...

Page 38: ... traveler nut Kink or blockage in tubing from Wash and Rinse reservoir to the syringe valve Clear inlet tubing of all obstructions Reset syringe plunger Wash and rinse reservoir inlet filter clogged Backflush or replace filters The syringe motor assembly needs to be replaced Contact your distributor for assistance See Appendix 2 13 E02 displayed Syringe plunger has become disconnected or jammed Se...

Page 39: ...steps that are available Check program and calibration parameters and Logic PCBA E 4 No room to Limit A stepper motor reached its Limit opto before the requested move can be completed Check program and calibration parameters and the Logic PCBA E 5 Limit error A stepper motor reached its Limit opto before the requested move towards Limit was completed Check the stepper motors stepper motor drive ci...

Page 40: ...5 4 USER TROUBLE SHOOTING NOTES Multiwash III 35 ...

Page 41: ...volume 3µL per well Liquid contact materials Glass Nylon Stainless Steel Polypropylene Tygon Teflon Delrin Polyethylene Santoprene Available upon request 6 2 MECHANICAL ELECTRICAL Dimensions h x w x d 9 5 h x 11 5 w x 16 0 d 242 x 292 x 406mm Weight approximate 12 lbs instrument only 21 lbs domestic shipping wt 25 lbs international shipping wt Power requirements 99 132VAC 198 264VAC 50 60Hz Power ...

Page 42: ...rranty In the event that the product is inoperable please call or fax your distributor and they will provide full failure details When making your call please ensure that you have the serial number of the instrument In the event that you are requested to return the instrument or any parts thereof IT IS YOUR LEGAL REQUIREMENT to ensure that the instrument is fully DECONTAMINATED see section 4 10 an...

Page 43: ...s Start Stop Press Start to begin processing the plate Press Stop to cancel the wash Press Stop again to return to the manifold to the soaking microstrip well The Prime key is used to prime the syringe pump lines and manifold with wash solution The Rinse key is used to rinse clean the syringe pump lines and manifold when the rinse tubing is connected to distilled water Step 2 Select of Rows Press ...

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