Revised 07/12/11
5.1
EMBEDDING PROCEDURE
EMBEDDING PROCEDURE
(Example)
NOTE
: The following procedure is an example
only. You may prefer to use your own method.
Before proceeding, make sure:
Q
both modules are properly set up according to
instructions in Section 3 (and Section 4, if auto-
matic timer is used)
Q
both modules are ready to use (check temperature
indicators, paraffin volume, base molds, paraffin
tray)
Q
tissues have been processed and are in the paraffin
bath of the Embedding Module.
4.
Select (and remove from heated chamber) one
base mold that would best fit the tissue(s) in the
cassette. Place under the paraffin dispenser.
5.
While holding base mold under paraffin dis-
penser, press fingerplate or use the optional
pedal to dispense only enough paraffin to half-
fill base mold.
6.
Place base mold on hot plate under paraffin dis-
penser.
7.
Using forceps, place tissue(s) from cassette into
base mold.
If tissues do not sink to bottom of mold, use
tampers or forceps to lightly push tissue(s)
down (into proper position) on the bottom of
base mold.
8.
Move the base mold to the cold spot. Bottom of
the base mold rapidly cools and a thin layer of
paraffin solidifies.
NOTE:
If the tissue is not correctly positioned,
move base mold back to hot plate; then use for-
ceps to reposition tissue(s). Return base mold
to cold spot. Repeat this procedure as necessary
until tissue(s) are properly oriented.
NOTE:
Never allow paraffin to completely
solidify during this process.
9.
Place one cassette over properly positioned tis-
sue(s) in base mold.
Embedded tissue(s) adhere to cassette.
Section 5
Embedding Procedure
(example)
1
.
Press LIGHT key to turn on Work Light.
Optional:
Properly position magnifying glass for best
view
2. Using heated forceps, remove one cassette
from the paraffin bath and place on either
right or left hot plate (depending on your
work flow direction).
3. Remove cassette cover, if required.