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Tissue-Tek TEC 5100, Operating Manual

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Tissue-Tek TEC 5100 Operating Manual Download Page 2

           IMPORTANT NOTICE

If this equipment is used in a manner not

specified by the manufacturer, the protection 

provided by the equipment may be impaired.

© 2011 Sakura Finetek USA, Inc.

All Rights Reserved

Printed in U.S.A.

 

 

 

 

 

 

 

 

 

 

 

Manufactured for:

 

 

Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.

 

 

Sakura Finetek Japan Co., Ltd., Tokyo, 135-0007, Japan

 

 

Sakura Finetek Europe B.V., 2804 AV Alphen aan den Rijn, NL

 

 

 

 

 

 

0001338-02 Rev.C

Summary of Contents for TEC 5100

Page 1: ...Operating Manual Tissue Embedding Console System Tissue Tek TEC 5 MODEL NUMBERS 5100 115 VAC America 5229 230 VAC Europe 5232 220 240 VAC Asia 5235 115 VAC Asia 5238 100 VAC Asia ...

Page 2: ...ction provided by the equipment may be impaired 2011 Sakura Finetek USA Inc All Rights Reserved Printed in U S A Manufactured for Sakura Finetek USA Inc Torrance CA 90501 U S A Sakura Finetek Japan Co Ltd Tokyo 135 0007 Japan Sakura Finetek Europe B V 2804 AV Alphen aan den Rijn NL 0001338 02 Rev C ...

Page 3: ...ch Magnifying Lens Assembly 3 2 Verify Proper Installation System Functioning Power On 3 3 Access Set Up Mode 3 4 Set Day and Time 3 4 Set Cryo Module Mode of Operation 3 4 Input Cryo Offset Valve 3 4 Change or View Set Temperatures 3 5 Add Paraffin 3 6 Place Base Molds into Chamber 3 6 What Happens When Power Is Turned Off 3 7 When To Set Power Switches On Front Panel To Off 3 7 4 AUTOMATIC TIMER...

Page 4: ... MINOR REPAIRS Instrument Care 7 1 Routine Maintenance Chart summary 7 1 Daily Maintenance 7 2 Disinfection 7 2 Periodic Maintenance As Required 7 3 Minor Repair 7 6 8 SERVICE AND REPLACEMENT PARTS Service 8 1 Replacement Parts 8 3 Accessories 8 3 Other Available Equipment and Accessories 8 4 TABLE OF CONTENTS ...

Page 5: ...pad and LCD display for easy access Q Two 3 hole forceps holders located on each side of the paraffin dispenser for right or left hand operations Q Menu driven software simplifies setup of the Automatic Timer you specify the time you require paraffin to be ready The instrument cal culates the time it should automatically turn the heaters on Q Embedding and Cryo Modules work areas of the same heigh...

Page 6: ...remaining four areas begin to heat 60 minutes prior to the preset ready time During that time the instrument status will be WARMING UP When all set temperatures are reached the instrument status will change to READY The module automatically turns OFF at your preset Shut down time or if you select the MANUAL mode and press HEAT May be longer depending on current source and environmental factors Cry...

Page 7: ...mps 60 Hz 9 0 amps 60 Hz Model Nos 5229 5232 220 240 VAC 10 or 220 240 VAC 10 or less 2 0 amps 50 Hz less 9 0 amps 50 Hz Chamber Capacity Paraffin Chamber approx 4 liters no chambers Left and Right Chambers approx 1 5 liters Temperature Range Paraffin Chamber Right and Cooling Plate Available Left Chambers Hot Plate 10 C to 0 C 50 C 75 C Electric Forceps optional 1 to 5 Factory Set Temperature Par...

Page 8: ......

Page 9: ...Cable to Embedding Module Embedding Module Power Cord Operating Manual not shown instructions for both Embedding and Cryo Modules Q Use extreme care when unpacking each module Each module contains sensitive electronic parts Check each item for shipping damage Q If there is visible shipping damage immediately file a complaint with the carrier Then notify your nearest Tissue Tek instrument distribut...

Page 10: ...or shown only connects underneath the control panel Adjustable to 5 different temperature lev els Forceps Warmer Holders hold forceps can be set to any temperature at or between 50 75 C 122 167 C using the Hot Plate temperature settings factory set at 62 C 149 F Paraffin Dispenser molten paraffin is dis pensed here press fingerplate to dispense paraf fin or use the optional foot pedal turn knob cl...

Page 11: ...affin bath Waste Drawers catch molten paraffin that drains through two holes in grooves around hot plate area Cold Spot cools liquid paraffin at the bottom of a base mold so tissue can be correctly posi tioned Hot Plate ensures that paraffin does not solidi fy during embedding process can be set to any temperature at or between 50 75 C 122 167 F factory set at 62 C 143 F This temperature setting i...

Page 12: ... Power Cord carries electri cal current to Embedding Module Tampers 1 large and 1 small used as neces sary to press tissue firmly against the bottom of the base mold Plastic Scraper used to remove frost from cooling plate of Cryo Module 2 4 Revised 07 12 11 UNPACKING SYSTEM DESCRIPTION ...

Page 13: ...c and manual operation The corresponding LED will be lit to indicate the current mode of opera tion Selecting the Manual Mode of operation does not permanently cancel the automatic timer On Off times LIGHT key turns Work Light ON or OFF CRYO key turns the Cryo Module ON or OFF The independent operation of the CRYO Module can be restricted if the Automatic Timer has been programmed to also control ...

Page 14: ... EXIT key allows to return to the previous menu item without saving the changes made in the SETUP mode and keys increase or decrease numerical values and add or delete days when in the SETUP mode Holding these keys for more than 2 seconds enables rapid scrolling ENTER key when pressed stores data into memory in the SETUP mode Display Adjustment Wheel controls the con trast for the display ...

Page 15: ...Revised 07 12 11 2 7 ...

Page 16: ......

Page 17: ...This allows you to place the modules next to each other in any order to accommodate your choice of right to left or left to right work flow 3 Check to make sure the power switches on the front panel of the Embedding Module and the Cryo Module are set to OFF O 4 Connect the Cryo Module to the Embedding Module using the provided cable The end of this cable having the right angle connector is plugged...

Page 18: ...the threaded hole of the paraffin dispenser The end of the flexible attach ment screws directly into the hole Tighten 3 Adjust magnifying lens to preferred focal range pull lens forward and down CONNECT ELECTRIC FORCEPS sold separately refer to section 8 1 Connect the wire underneath the Embedding Module control panel 3 2 Revised 07 12 11 SET UP ...

Page 19: ...D dis play 4 Press CRYO key on Embedding Module control panel The CRYO IS OFF message will disappear from the display and the compressor will start If System functions properly Q press CRYO key to turn off compressor the mes sage CRYO IS OFF is back on the display Q continue with Set Day and Time procedure next page NOTE Paraffin will not be dispensed and an audi ble alarm will sound if the finger...

Page 20: ...the Embedding Module when the MAN AUTO keys is set to MANUAL MAN LED is illuminated The CRYO key can then be used to turn the Cryo Module ON or OFF NOTE The Cryo Module power switch must be turned ON first The Embedding Module power switch must then be ON to operate the Cryo module When AUTOMATIC operation is selected AUTO LED is illuminated control of the Cryo Module ON and OFF times can be synch...

Page 21: ...d keys to set the temperatures When finished press ENTER to save the values into memory or EXIT to discard the changes Temperature can be set for the following compo nents in 1 C increments 1 Paraffin Paraffin Chamber from 50 C to 75 C 2 Cryo Cryo Module from 10 C to 0 C 3 Hot Plate Hot Plate from 50 C to 75 C The Hot Plate setting also controls the Forceps Warmer Holders 4 Left Left Chamber from ...

Page 22: ...in to either right or left chamber whichever you select depending on your work flow direction added to increase heat conductivity between tray and chamber Place transfer tray into the chamber over 50 mL molten paraffin 4 Add paraffin to the paraffin bath to a depth of about 13 mm 0 5 in or about 9 liter 24 gal molten paraffin or equivalent in dry chips flakes 4 NOTE The paraffin bath may be either...

Page 23: ...errupted or turned off while the compressor is ON The fan will start immediately When the Cryo Module is intermittently not in use during the day turn the compressor OFF by pressing the CRYO key on the Embedding Module control panel This action will not be allowed if the Cryo Module operation was set to synchronize with the Embedding Module and the mode of operation selected is AUTO AUTO LED is il...

Page 24: ......

Page 25: ...gram the Automatic Timer access the SET UP mode by pressing the SET key and highlight menu item 3 TIMER Press ENTER The fol lowing information will be displayed Days already programmed will be annoted by a symbol The current day of the week will be high lighted In the above example the Automatic Timer will turn on and off the TEC 5 from Monday to Friday but not during the weekends the current day ...

Page 26: ...ing mode is AUTO AUTO LED is illu minated and the system is in Standby the following information will be displayed When the instrument is in STANDBY all heaters are off and the Cryo module is off To turn the heaters on before the next scheduled ON cycle press the HEAT Key To turn the Cryo Module ON press the AUTO MAN key to illuminate the MAN LED then press the CRYO key NOTE The CRYO key can be pr...

Page 27: ... Using forceps place tissue s from cassette into base mold If tissues do not sink to bottom of mold use tampers or forceps to lightly push tissue s down into proper position on the bottom of base mold 8 Move the base mold to the cold spot Bottom of the base mold rapidly cools and a thin layer of paraffin solidifies NOTE If the tissue is not correctly positioned move base mold back to hot plate the...

Page 28: ... humidity This enhances heat exchange between the warmed base mold and the cooling plate 13 Check to make sure the paraffin block is com pletely solidified then remove paraffin block from base mold NOTE The paraffin block should easily release from the base mold If not the base mold may need cleaning Use of the Tissue Tek Mold Release Concentrate product code 4141 will make demolding easier 14 If ...

Page 29: ...e does not reach set temperature 6 2 Power indicator will not illuminate 6 2 Embedding Module Page Alarm sounds and error message is displayed 6 3 Paraffin will not dispense correctly 6 4 Power indicator other indicators will not illuminate 6 4 In the event this section cannot assist you in solving your instrument problem call 1 800 725 8723 U S Customers only If located outside the U S contact yo...

Page 30: ... to make sure function or compressor has Q the power cord is connected to a live outlet malfunctioned Q the connecting cable is securely attached to the Q Cryo and the Embedding Modules Q the power switch on the front panel of the Embedding Module is set to ON and power indica tor is illuminated Q the CRYO key on the Cryo Module control Q panel is ON If the problem persists contact Customer Suppor...

Page 31: ...the cursor to highlight the next error code number and record the error indication Embedding Module Check to make sure status never reaches Q if you have recently added cold paraffin base READY Q molds install a cool forceps warmer etc Q that may have caused more than a 5 C drop Q in an area temperature If so wait a few minutes Q for the temperature to stabilize and the error Q condition will then...

Page 32: ...take longer depending on current source and environmental factors Paraffin filter may If paraffin is molten and set temperature has been need cleaning reached paraffin flow may be obstructed by a clogged paraffin filter Refer to Section 7 for cleaning instructions If the problem persists contact Customer Support Power indicator other Power outage or Check to make sure indicators will not Embedding...

Page 33: ...ng used turn OFF the compressor use CRYO key on control panel Q Do not place anything on the top panels of either module Placing items on the top panels interferes with proper ventilation and heat exchange Q WARNING MOLTEN PARAFFIN MAY CAUSE BURNS DO NOT MOVE THE EMBEDDING MODULE WHEN THE PARAFFIN CHAMBER OR PARAFFIN BATH IS FILLED WITH MOLTEN PARAFFIN TURN THE POWER OFF THEN ALLOW THE PARAFFIN TO...

Page 34: ...solidified paraffin from the gray insulated work space and hot plate area Q As necessary wipe the insulated work space and hot plate area with a xylene dampened cloth Q Clean the grooves surrounding the hot plate areas with a xylene dampened cotton swab Cryo Module Turn off compressor press CRYO key on Embedding Module Control Panel Allow cooling plate to reach ambient temperature Remove condensat...

Page 35: ...d transfer tray to liquefy just enough to be able to remove tray remove tray Press the HEAT key OFF 3 Turn tray upside down over a waste container and slightly twist tray to eject solidified paraffin 4 WARNING BEFORE PROCEEDING MAKE SURE THE HEAT KEY IS OFF Allow paraffin in chamber where tray was locat ed to solidify Using the plastic scraper remove as much solidified paraffin as possible from th...

Page 36: ...th and paraf fin chamber use plastic scraper Using a clean cloth remove loose debris from the chamber used to store heat base molds 7 Using a xylene dampened cloth wipe exterior surfaces ventilation panels and inside surfaces of paraffin chamber paraffin bath base mold storage chamber and waste drawer Wipe each forceps well with a xylene dampened cotton swab WARNING XYLENE IS FLAMMABLE BE SURE THE...

Page 37: ...rticles and other non paraffin debris 3 Dry entire assembly using a soft clean dry cloth To remove paraffin debris 1 Dampen a soft cloth with xylene 2 Wipe assembly to remove paraffin and other debris 2 WARNING XYLENE IS FLAMMABLE BE SURE THE MODULE IS COOL PRIOR TO USING XYLENE 2 CAUTION Do not immerse assembly in xylene or frame surrounding lens may soften or disintegrate 3 Dry assembly using a ...

Page 38: ...ar plastic shield protecting lights 2 Grasp light bulb and pull downward to remove light bulb Repeat procedure for other light bulb if it also requires replacement WARNING Avoid touching hot plate 3 Grasp new light bulb and insert upwards into socket Repeat as required for other light bulb 4 Plug power cord into wall outlet Set power switch on front panel to ON 5 Press LIGHT key 5 NOTE To extend l...

Page 39: ...CE AND REPLACEMENT PARTS SERVICE When you have a problem with either module Q Refer to Troubleshooting Section 6 If Section 6 cannot assist you in solving the prob lem continue with How To Report the Problem next page Section 8 ...

Page 40: ...r message displayed Yes No 6 Does the LCD display Yes No function 7 Do keys properly function Yes No CRYO MODULE 1 Is the cryo module Yes No power cord plugged in 2 Is the power switch on the front Yes No panel of both Modules set to ON 3 Are the POWER indicators lit Yes No 4 Is the connecting cable linking Yes No both modules inserted properly at both ends 5 Does the cooling plate reach Yes No th...

Page 41: ...7 100 VAC Asia 5240 part numbers are subject to change without notice ACCESSORIES Available for use with your Tissue Tek Tissue Embedding Console System Tissue Tek Uni Cassette Embedding Cassettes Tissue Tek II Processing Embedding Cassettes Tissue Tek Base Molds Tissue Tek Mold Release Concentrate Product Code No 4141 12 case Magnifying Lens Assembly Product Code No 5784 Foot Pedal Product Code N...

Page 42: ...ambient air and keeps solvent fumes out of the working area Tissue Tek Timing Disk provides for Rotary automatic processing Tissue Processor handles approximately 100 specimens at a time Equipment and Accessories Features Tissue Tek Available for use with Uni Cassette mechanical imprinters and for manual identification 11 distincitive colors Tissue Tek has 1 mm pores no slots Biopsy Cassette reduc...

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