Thermo Scientific Vitrobot Mk IV, User Manual

Page: 27 / 38

Chapter | Operating Instructions
Confidential, limited rights
PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 27
For manual application of the sample onto a grid, select with the foot pedal switch or the
stylus ‘Continue’ to proceed. The tweezers is slightly lowered as to allow the application of
suspension through the side-entry port using a pipette. As a consequence of this method only
one side of the grid is inoculated with suspension. The advantage of this method is th at only
small volumes of the sample (typically 3 µl) are used.
3.8 Blotting and Vitrification
The excess of suspension must be removed from the grid prior to plunge freezing. To do so,
select ‘Continue’ in the UI or use the foot pedal. This activates a slight uptake of the grid
towards the correct position between the blot pads and a subsequent blotting of the grid. The
blotting conditions can be set in the ‘Options page’ of the UI (. After each blot, the blot pads
undergo a slight rotation to ensure a clean, new area of filter paper for the next blot session.
For details about the UI see:
Starting Up
– Switching on the Vitrobot on page
17
Note After a maximum of 16 blottings, the filter papers must be replaced. The UI displays
the message: ‘Replace Blotpapers’.