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SORVALL

®

Centrifuges

ii

This manual is a guide for use of the

SORVALL

®

RC-5C PLUS Superspeed Centrifuge

Data herein has been verified and is believed adequate for the intended use of the centrifuge. Because
failure to follow the recommendations set forth in this manual could produce personal injury or property
damage, always follow the recommendations set forth herein.

Thermo

does not guarantee results and

assumes no obligation for the performance of products that are not used in accordance with the
instructions provided. This publication is not a license to operate under, nor a recommendation to
infringe upon, any process patents.

Publications prior to the Issue Date of this manual may contain data in apparent conflict with that
provided herein. Please consider all data in this manual to be the most curent.

WARNING

,

CAUTION

, and

NOTE

within the text of this manual are used to emphasize important

and critical instructions.

WARNING

informs the operator of a hazard or an unsafe practice that could result in personal

injury, affect the operator's health, or contaminate the environment.

CAUTION

informs the operator of an unsafe practice that could result in damage of equipment.

NOTE

highlights essential information.

CAUTION

and

WARNING

are accompanied by a hazard symbol

!

and appear in the left

sidebar near the information they correspond to.

© 1996, 1999, 2000, 2003 by Thermo Scientific

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Summary of Contents for SORVALL RC-5C PLUS

Page 1: ...Analyze Detect Measure Control RC 5C Plus User s Manual PN 74559 9 ...

Page 2: ...PN 74559 9 Issued February 2007 OPERATING INSTRUCTIONS SORVALL RC 5C PLUS Superspeed Centrifuge Thermo Scientific Asheville North Carolina U S A ...

Page 3: ...tion to infringe upon any process patents Publications prior to the Issue Date of this manual may contain data in apparent conflict with that provided herein Please consider all data in this manual to be the most curent WARNING CAUTION and NOTE within the text of this manual are used to emphasize important and critical instructions WARNING informs the operator of a hazard or an unsafe practice tha...

Page 4: ... compartment load specified See Chapter 4 Operation Failure to reduce rotor speed under these conditions can cause rotor failure Centrifuges routinely deal with high energy levels and could move suddenly in the unlikely event of rotor failure During centrifuge operation never lean on or move the centrifuge keep the surrounding area clear of objects including all hazardous materials and do not work...

Page 5: ... 2 2 Location Requirements 2 2 Installation 2 3 Chapter 3 CONTROLS DISPLAYS INDICATORS Controls Displays Indicators 3 1 Key Switch and Power Switch 3 2 Temperature Switches and Display 3 2 Speed Switches Display and Indicator 3 2 Time ω2 dt Switches Display and Indicator 3 3 Rotor Code Switches 3 3 TIMED HOLD Switch 3 3 OFF BRAKE Switch 3 4 START Switch 3 4 STOP Switch 3 4 RPM RCF Switch 3 4 OFF A...

Page 6: ...cing Speed for Loads in Excess of Design Mass 4 8 RCF Calculation 4 9 Test Run for Low Speed and Low Temperature Operation 4 10 Entry Errors 4 11 Chapter 5 CARE AND MAINTENANCE Maintenance 5 1 Cleaning and Decontamination 5 2 Lubrication 5 2 LED and Indicator Test 5 3 Parts Ordering Information 5 3 Emergency Sample Recovery 5 4 Service Decontamination Policy 5 4 APPENDIX Rotor Information Table Wa...

Page 7: ...1 Centrifuge Parts Location and Identification 1 2 3 1 Control Panel 3 1 4 1 Integral Value 4 6 5 1 Door Latch Override Button Location 5 4 List of Tables Table Page 4 1 Rotor Codes 4 3 5 1 Maintenance Schedule 5 1 Table of Contents Table of Contents continued ...

Page 8: ...l compatible SORVALL superspeed rotors Integrator ω2 dt It can be used either as a control or a display or both When used as a control the centrifuge will shut down based on a preselected integral value rather than a preselected time and when used as a display the accumulated integral value will be displayed during and after a timed run Automatic rate controlled slow start and slow stop Refrigerat...

Page 9: ...them The refrigeration system charged with environmentally friendly CFC free SUVA refrigerant offers reliable performance similar to earlier SORVALL superspeed centrifuges The high capacity refrigeration system is a low temperature hermetically sealed unit that consists of a compressor a condenser an evaporator rotor chamber and interconnecting tubing During operation the refrigeration system will...

Page 10: ... phase 30 A 240 V 50 Hz 230 V 60 Hz 230 V 50 Hz 220 V 50 Hz 208 V 60 Hz 200 V 60 Hz 200 V 50 Hz Input Power polyphase 30 A 230 V 50 Hz 1 Speed in revolutions per minute rpm is related to angular velocity ω according to the following ω rpm rpm 0 10472 Where ω rad s All further references in this manual to speed will be designated as rpm 2 May vary at very low speeds below approximately 2000 rpm 3 T...

Page 11: ...ingle phase 3 pin IEC 60309 for all other single phase NEMA 6 30R for NEMA 6 30P grounded plug supplied for 230 V 50Hz polyphase 5 pin IEC 60309 Centrifuge Accessories The following items are provided with the centrifuge Catalog No Description 74567 Condensed Operating Instructions 74559 Instruction Manual 68025 9 16 inch Wrench 91499 NORMAL ZONAL Key ...

Page 12: ...phase 230V 50Hz 32A polyphase 220V 50Hz 30A single phase 240V 50Hz 30A single phase Check the supply line voltage with a voltmeter then verify that the voltage indicated on the nameplate is in agreement with the mea sured line voltage If the measured line voltage is not within 10 of the voltage specified on the nameplate do not connect the power cord and operate the centrifuge Damage to the centri...

Page 13: ...Locate the RC 5C PLUS centrifuge on a level floor In addition to considering ambient temperature see environment requirements above allowing adequate space for air circulation is important for the centrifuge to function properly To ensure free air circulation the centrifuge must be positioned so that no air vents are blocked in a location that allows an additional 10 cm 4 inches clearance from all...

Page 14: ...otor on the drive spindle WITHOUT its lid Place a level on the center hub of the rotor 5 Using the 9 16 inch wrench provided alternately turn the two front feet to raise or lower the front corners of the centrifuge until the centrifuge is level and both feet bear weight Read the CAUTION WARNING 6 Remove the level and the rotor from the rotor chamber 7 Plug the centrifuge into the appropriate elect...

Page 15: ......

Page 16: ... let you know when the centrifuge is not functioning properly Refer to figure 3 1 for the location of the controls displays and indicators described in this chapter Figure 3 1 RC 5C PLUS Control Panel SPEED RPM TIME HRS MIN RPM RCF COEF EXP w2dt HRS MIN ROTOR CODE ACCESSORY MODE NORMAL MODE OFF TIMED OFF START STOP RPM RCF OFF ARC HOLD BRAKE OUT OF BALANC C OVER TEMP OVER SPEEE BRUSHES DOOR FAULT ...

Page 17: ...erature Switches and Display The temperature display indicates estimated sample temperature The RUN temperature switch is used to set desired sample temperature The MAX temperature switch is used to set the overtemperature which is the maximum permissible sample temperature If the sample temperature exceeds this limit the centrifuge will shut off and the OVERTEMP message will light Speed Switches ...

Page 18: ...the START switch is pressed The centrifuge will shut off when the preselected value is achieved provided the TIMED HOLD switch is set to TIMED if the switch is set to HOLD the run will continue until STOP is pressed Rotor Code Switches The code number of the rotor in use must be set on these switches for proper automatic temperature compensation speed control and RCF calculation At the beginning o...

Page 19: ...g a run the SPEED display numbers will change from the current rotor speed to the current relative centrifugal force RCF commonly referred to as g force value To be sure that the RCF value is correct make sure the proper rotor code number is set on the ROTOR CODE switches When the RPM RCF switch is released the display will change back to show rotor speed This switch can also be used to calculate ...

Page 20: ...ue is reached If the OFF ω2 dt switch is set to OFF when the START switch is pressed the centrifuge will operate in either a timed mode or a continuous run HOLD mode depending on the setting of the TIMED HOLD switch The TIME display will show either remaining run time TIMED or accumulated run time HOLD During a TIMED run the accumulated integral value can be displayed by changing the setting of th...

Page 21: ...ed the desired maximum run temperature selected on the MAX temperature switch Some problems that could cause this are run temperature setting may be too low for the rotor speed combination check rotor instruction manual to verify temperature setting room ambient temperature is above 25 C or inadequate air circulation see Location Requirements page 2 1 Check each of these possible causes before not...

Page 22: ... Blinks on and off whenever a diagnostic indicator lights up the indicator represents a condition that has caused the run to end diagnostic indicators number 1 8 See Diagnostic Indicators later in the chapter ENTRY ERROR Lights when a wrong entry has been made on one of the control settings See page 4 11 for a list of possible entry errors ω2 dt Indicates that you have selected the integral w2 dt ...

Page 23: ... Engineer MEMORY SYSTEM FAILURE Notify Thermo Field Service Engineer DRIVE SYSTEM FAILURE Notify Thermo Field Service Engineer NO ROTOR The microcomputer has detected a sudden and sustained increase in rpm because there was no rotor installed when the START switch was pressed The centrifuge ran for a few seconds then shut off Install a rotor and restart the run REFRIGERATION SYSTEM FAILURE There w...

Page 24: ...mentary If the condition persists notify your Thermo Field Service Engineer REGISTER OVERFLOW This problem could be momentary if it is the condition will clear and the light will go out Also a wrong entry on one of the control switches may cause this light to come on if the ENTRY ERROR message is lit check all control settings If the condition persists notify your Thermo Field Service Engineer NOT...

Page 25: ......

Page 26: ...ol ARC Operation To dynamically load a zonal rotor see Zonal Mode Operation To control a run with the integrator see Integrator Mode Operation To perform a normal centrifuge run the centrifuge power must be set ON and a rotor must be loaded and installed according to the instructions in the rotor manual read the WARNING and the CAUTION If the sample temperature during the centrifuge run is critica...

Page 27: ... MAX temperature setting 3 Set the key switch on the front panel to NORMAL 4 Open the chamber door 5 If applicable make sure the rotor cover is installed on the rotor and for fixed angle rotors secured 6 Place the rotor on the drive spindle and lock it in place by turning the rotor locking knob counterclockwise 7 Set the ROTOR CODE switches for the proper ROTOR CODE number see Table 4 1 8 Close th...

Page 28: ...ll end the run will still end when the preselected time has elapsed 14 After the rotor has come to a stop and the door has unlatched open the chamber door and remove the rotor Read the CAUTION NOTE We recommend keeping the chamber door closed after the rotor has been removed to inhibit the formation of condensation on the chamber walls The run can be repeated with the same parameters by installing...

Page 29: ... the TZ 28 rotor according to the instructions in the rotor instruction manual then NOTE If desired precool the rotor in the centrifuge according to the procedure on page 4 7 Read the WARNING and the CAUTION 1 Set the main power switch to ON 2 Set the key switch on the front panel to ZONAL 3 Press the door release button and open the chamber door 4 Install the rotor as instructed in the rotor manu...

Page 30: ... ROTOR CODE advisory message will blink for a few seconds as a reminder to set the proper ROTOR CODE number NOTE The timer will start as soon as you press the START switch therefore include the time it will take to load the rotor in the run time you select 2 Wait for the rotor to reach the selected loading speed then load the rotor according to the instructions in the rotor instruction manual When...

Page 31: ...the integrator contact the SORVALL Applications Laboratory The RC 5C PLUS allows you to obtain the integral value generated during a normally timed run by pressing the OFF ω2 dt switch to ω2 dt when the run has stopped see Integral Recall The integral value can then be used to control subsequent centrifuge runs This procedure specifies how to set up a run to be controlled by an integral value Prep...

Page 32: ...any TIMED run normal or zonal mode you can recall the integral value ω2 dt at timeout and use this value in the integrator ω2 dt mode to duplicate the run When the rotor has stopped change the setting of the OFF ω2 dt to ω2 dt the integral value at timeout will appear in the TIME display The value must be recalled before another run is started or before the main power is set OFF Rotor Precool If y...

Page 33: ...the rotor lid Lock the rotor to the drive spindle Close the chamber door 11 Perform the desired centrifuge run Reducing Speed for Loads in Excess of Design Mass There is a maximum allowable compartment mass established for each rotor see the Rotor Information Table in the Appendix or the individual rotor manual To prevent rotor failure the total contents of any compartment including specimen tubes...

Page 34: ...ld the RPM RCF switch down to RCF 4 The SPEED display will show the RCF value for the identified rotor at the speed that is set on the RPM switches 5 Adjust the speed setting on the RPM switches until the desired RCF value appears in the SPEED display the speed set on the RPM switches is the speed at which you have to run that rotor to achieve the displayed RCF NOTE If the ENTRY ERROR advisory mes...

Page 35: ... them in the rotor Balance the rotor according to the instructions in the rotor instruction manual NOTE The dispensable fluid should have a freezing temperature well below the desired sample temperature 2 Set the RUN temperature switch to the desired sample temperature 3 Install the rotor in the centrifuge and run it for at least two hours at the speed for which the temperature setting is being de...

Page 36: ... speed setting The RPM switches are set at or above 105 of the maximum speed of the identified rotor Check the ROTOR CODE setting to make sure you have selected the proper code number and check the speed setting to make sure it is within range for the rotor The TIMED HOLD switch is set at TIMED the OFF ω2 dt switch is set at OFF and the TIME HRS and MIN switches are set at zero The run will not st...

Page 37: ......

Page 38: ... continuity tested Once a year or whenever centri fuge is serviced Clean the air inlet on the drive Once a month box wipe with cloth Chapter 5 CARE and MAINTENANCE This chapter describes routine maintenance procedures for your RC 5C PLUS Centrifuge It also includes cleaning and decontamination recommendations and the Service Decontamination Policy As the user it is your responsibility to make cert...

Page 39: ... nonalkaline detergent and water Do not use abrasive cleansers Refrigeration System Condenser Fins To maintain the efficiency of the refrigeration system the condenser fins located in the rear of the centrifuge must be kept free of dust and dirt Condenser fins should be cleaned with a brush or a vacuum cleaner at least annually more often if dust accumulates significantly The fins will bend easily...

Page 40: ...sory messages should light diagnostic indicators 1 through 12 should light the ω2 dt indicator should light NOTE The RCF indicator will not light to test this indicator just press the RCF switch If any of the LEDs or indicators are not working contact a Thermo Field Service Engineer to repair it Parts Ordering Information To order replacement parts in the United States call Thermo toll free 1 800 ...

Page 41: ... pencil or similar object and at the same time push the door button The chamber door will open NOTE The chamber door will not open if the door latch override button and door button are not pushed at the same time Service Decontamination Policy If a centrifuge or rotor that has been used with radioactive or pathogenic material requires servicing by Thermo personnel either at the customer s laborato...

Page 42: ...icer can decide whether to authorize the rotor s return to a Thermo facility Do not leave a loaded rotor locked inside a centrifuge that requires servicing If with a loaded rotor installed in the cham ber a centrifuge malfunction makes it so that the chamber door will not open by normal means follow the Emergency Sample Recovery procedure found in your centrifuge operating instruc tions manual to ...

Page 43: ...om your Thermo representative NOTE United States federal regulations require that parts and instruments must be decontaminated before being trans ported Outside the United States check local regulations If equipment is received at Thermo facilities without a valid RMA Number on the outside of the shipping container and a completed Equipment Return Decontamination Form on file the equipment will be...

Page 44: ...RC 5C PLUS Rotor Information Table APPENDIX Appendix ...

Page 45: ... 45 959 514 30 9 33 3 81 HB 4 07 13 000 1 100 27 712 1 667 250 14 68 4 82 HS 4 08 7 000 1 000 9 430 4 491 1 035 17 23 7 22 SM 24 09 20 000 1 100 49 461 2 456 2 27 11 07 2 5 38 2 TZ 28 11 20 000 900 42 580 612 1 620 9 53 3 62 SH MT 12 13 730 3 1 400 19 141 3 657 3 36 4 9 09 3 5 57 3 SH 80 13 20 000 700 45 395 400 78 10 16 5 40 F 28 13 14 19 500 1 200 48 548 294 23 11 43 7 34 F 28 36 15 18 000 1 200...

Page 46: ...3000 buckets 29 4 350 900 3 919 9 500 1 805 18 54 9 11 SLA 3000 30 11 000 500 20 449 2 813 780 15 13 3 94 SH 3000 microplates 31 4 350 900 3 175 2 987 15 02 2 SLA 600TC 32 12 500 820 25 656 987 74 14 70 7 99 SLC 4000 33 7 000 400 9 184 7 655 1 500 16 76 2 51 SLC 1500 34 14 000 700 25 862 1 944 420 13 70 3 74 SLC 3000 35 10 000 650 17 604 3 394 780 15 76 4 12 SA 512 36 18 500 1 000 44 040 3 438 3 3...

Page 47: ......

Page 48: ...se abuse use of electri cal currents or circuits other than those specified on the plate affixed to the instrument accident negligence failure to follow operating instructions or use of any rotor other than a SORVALL rotor in tended for use in this instrument Thermo reserves the right to change alter modify or improve any of its instruments without any obligation whatsoever to make cor responding ...

Page 49: ...s activated by turning the key on the front panel to NORMAL and the OFF ARC switch and the OFF ω2 dt switch to OFF Overtemperature The maximum estimated sample temperature MAX allowed during a centrifuge run if the temperature in the display exceeds this value the run will terminate RCF Relative Centrifugal For ce An accessory that calculates RCF The force g for ce applied to a suspension of parti...

Page 50: ...Speed RCF 3 2 Temperature 3 2 Time ω2 dt 3 3 DOOR message 3 7 Door opening emergency 5 4 Electrical requirements 1 3 2 1 2 2 Emergency sample recovery 5 4 ENTRY ERROR message 3 7 4 11 Entry errors 4 11 Environment requirements 2 2 FAULT message 3 7 Heat output 1 3 HOLD message 3 7 Indicator test 5 3 Indicator ω2 dt 3 3 Advisory message backlit 3 6 3 7 Diagnostic number 1 thr ough 12 3 8 3 9 RCF 3 ...

Page 51: ...ble ROTOR CODE message 3 7 Rotor code switches 3 3 RPM switches 3 2 RPM RCF switch 3 4 Safety iii Service Decontamination Policy 5 5 5 6 Specifications centrifuge 1 3 Speed maximum centrifuge 1 1 1 3 Speed RCF display 3 2 Speed switches 3 2 START switch 3 4 STOP switch 3 4 Temperature ambient 1 3 2 1 Temperature display 3 2 Temperature switches 3 2 Test LED and indicator 5 3 Test run low speed and...

Page 52: ..._________________ INSTRUMENT ____________________________________________________________________ SERIAL NUMBER _____________________________________ ROTOR _________________________________________________________________________ SERIAL NUMBER _____________________________________ PART ______________________________________________________________________________________ PART NUMBER ______________...

Page 53: ...cal hazard our represen tative will not service the equipment until proper decontamination and certification has been completed If an instrument is received at our Service facilities and in our opinion poses a radioactive or biological hazard the sender will be contacted for instructions as to disposition of the equipment Disposition costs will be borne by the sender Decontamination Information Ce...

Page 54: ...Sie unter www thermo com WEEERoHS Conformità WEEE Questo prodotto deve rispondere alla direttiva dell Unione Europea 2002 96EC in merito ai Rifiuti degli Apparecchi Elettrici ed Elettronici WEEE È marcato col seguente simbolo Thermo Scientific ha stipulato contratti con una o diverse società di riciclaggio smaltimento in ognuno degli Stati Membri Europei Questo prodotto verrà smaltito o riciclato ...

Page 55: ...es autres pays Contactez votre concessionnaire local des produits SORVALL ou leur representant AUSTRIA Tel 43 1 80 14 00 FRANCE Tel 33 1 69 18 77 77 33 2 28 03 20 00 NETHERLANDS Tel 31 7 65 71 44 40 UNITED KINGDOM or IRELAND Tel 44 87 06 09 92 03 BELGIUM Tel 32 24 82 30 30 GERMANY Tel 49 61 84 90 69 40 49 61 03 40 81 012 SPAIN or PORTUGAL Tel 34 9 32 23 31 54 Other EUROPE MIDDLE EAST or AFRICA Tel...

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