Thermo Scientific SORVALL RC-5C PLUS, User Manual

Page: 29 / 55

Operation SORVALL ® Centrifuges
4-4
W A R N I N G
When loading the rotor, be
sure not to exceed the maximum com-
partment mass of the rotor (see the
Rotor Information Table in the Ap-
pendix). If maximum compartment
mass is exceeded, the maximum op-
erating speed must be lowered (see
Reducing Speed for Rotor Compart-
ment loads in Excess of Design Mass
later in this chapter). Failure to do so
can cause rotor failure, which could
result in centrifuge damage and per-
sonal injury.
!
C A U T I O N
Failure to load and install
the rotor in accordance with the rotor
instruction manual could result in
damage to the centrifuge. The rotor
cover must be on and locked in place
and the rotor must be locked to the
drive spindle.
!
C A U T I O N
Do not operate the rotor at
its critical speed (see the Rotor Infor-
mation Table in the Appendix).
!
Zonal Mode Operation
The zonal mode of operation must be used when dynamically
loading the gradient and sample into a SORVALL
® TZ-28 Zonal
Rotor. When the zonal mode is activated, the centrifuge will
accelerate to a low speed for rotor loading and when loading is
complete, accelerate to the set run speed.
Preparation for Loading
Assemble the TZ-28 rotor according to the instructions in the rotor
instruction manual, then:
NOTE
If desired, precool the rotor in the centrifuge according to
the procedure on page 4-7.
Read the WARNING and the CAUTION.
1. Set the main power switch to ON.
2. Set the key switch on the front panel to ZONAL.
3. Press the door release button, and open the chamber door.
4. Install the rotor as instructed in the rotor manual. Set the ROTOR
CODE switch to 11.
5. Set the RPM switch to the desired loading speed, which must be
between 50 and 1400 rpm (1400 rpm is the recommended speed).
Read the CAUTION.
6. Set the OFF/
∫ω
2
dt switch to OFF.
7. Set the RUN and MAX temperature switches to the desired run
temperature and maximum temperature. The MAX temperature
setting should be for a value that is at least 5
°
C higher than the
RUN temperature setting.
NOTE
For optimal temperature control at RUN temperatures
below ambient, both the rotor and the rotor chamber
should be at the desired RUN temperature before the
run is started. If the rotor was precooled in the centrifuge,
the chamber will already be at the desired temperature.
If the rotor was precooled by some other means, you
should wait until the temperature display indicates the
desired RUN temperature before starting the run. The
run will not start as long as the displayed temperature is
greater than the MAX temperature setting.