Thermo Scientific SORVALL RC-5C PLUS, User Manual

Page: 28 / 55

RC-5C PLUS Operation
4-3
10. Make sure the OFF/
∫ω
2 dt switch is set to OFF.
11. Set the TIMED/HOLD switch:
• For a timed run, set the switch to TIMED, then set the HRS
and MIN switches for the desired length of run time.
• For a continuous run, set the switch to HOLD (the setting on
the HRS and MIN switches is ignored). When HOLD is
selected, the HOLD message indicator light will come on.
12. Set the OFF/BRAKE switch to the desired setting (refer to
Chapter 3). Set the OFF/ARC switch to OFF.
13. Press the START switch. (The ROTOR CODE advisory message
will blink for a few seconds as a reminder to set the proper
ROTOR CODE number.)
NOTE
To end a run in HOLD or before the selected run time
has elapsed, press the STOP switch.
If you want to see the accumulated integral value anytime
during a TIMED run, change the setting of the OFF/
∫ω
2
dt
switch to
∫ω
2 dt
. As long as you change the setting of this
switch AFTER the START switch is pressed it will not
have any affect on how the run will end - the run will still
end when the preselected time has elapsed.
14. After the rotor has come to a stop and the door has unlatched,
open the chamber door and remove the rotor.
Read the
CAUTION.
NOTE
We recommend keeping the chamber door closed after
the rotor has been removed to inhibit the formation of
condensation on the chamber walls.
The run can be repeated with the same parameters by installing
the rotor, closing the chamber door, and pressing START. The selected
speed, time, and temperature are retained from previous run.
Rate Control (ARC) Operation
The automatic rate control (ARC) accessory provides a slow,
controlled acceleration from 0 to 512 rpm and a slow, controlled
deceleration from 768 to 0 rpm. The ARC should be used when
centrifuging gradients or fragile pellets. To do a rate-controlled run,
follow the Normal Mode Operation procedure, except set the OFF/
ARC switch to ARC in Step 12 to activate the rate control.
C A U T I O N
To reduce the possibility of
samples freezing, remove the rotor
from the centrifuge chamber as soon
as possible after it stops spinning.
!
W A R N I N G
Do not leave rotors or other
objects on centrifuge surfaces during
operation.
!