Thermo Scientific SORVALL RC-5C PLUS, User Manual

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RC-5C PLUS Operation
4-1
Chapter 4: OPERATION
This chapter provides step-by-step instructions on how to set the centrifuge power ON, open the
chamber door, and perform a run in the normal mode. It also describes the rate control, zonal, and
integrator modes of operation and how to precool the rotor.
Read and observe the Important Safety
Information supplied on page iii at the front of this manual.
The chapter contains supplementary information on how to calculate relative centrifugal force (RCF);
how to do a test run for low speed and low temperature operation; and what will cause an ENTRY
ERROR. The controls and displays referred to in this chapter are described in detail in Chapter 3.
Setting the Centrifuge Power ON
The centrifuge power ON/OFF switch is located in the upper-right
corner of the front cabinet panel, below the control panel (see figure
1-1). Press the top of the switch to set the centrifuge power ON.
Opening the Chamber Door
Press the door release button (located under the right-front corner
of the top deck, see figure 1-1) to r
elease the door latch and open the
chamber door.
Normal Mode Operation
The normal mode of operation should be used for most RC-5C
PLUS centrifuge runs. (EXCEPTIONS: To slowly accelerate or
decelerate fragile gradients or samples, see Rate Control [ARC]
Operation. To dynamically load a zonal rotor, see Zonal Mode
Operation. To control a run with the integrator, see Integrator Mode
Operation.)
To perform a normal centrifuge run, the centrifuge power must be
set ON and a rotor must be loaded and installed according to the
instructions in the rotor manual
(read the WARNING and the
CAUTION) . If the sample temperature during the centrifuge run is
critical, we recommend that you precool the rotor and chamber as
described on page 4-7 before beginning this procedure.
NOTE
The controls and displays referred to in this procedure
are defined in Chapter 3.
If the ENTRY ERROR message lights at any time during
this procedure, refer to page 4-11 for possible causes.
W A R N I N G
When loading the rotor, be
sure not to exceed the maximum com-
partment mass of the rotor (see the
Rotor Information Table in the Ap-
pendix). If maximum compartment
mass is exceeded, the maximum op-
erating speed must be lowered (see
Reducing Speed for Rotor Compart-
ment loads in Excess of Design Mass
later in this chapter). Failure to do so
can cause rotor failure, which could
result in centrifuge damage and per-
sonal injury.
C A U T I O N
Failure to load and install
the rotor in accordance with the rotor
instruction manual could result in
damage to the centrifuge. The rotor
cover must be on and locked in place
and the rotor must be locked to the
drive spindle.
!
!
W A R N I N G
DO NOT operate the GSA,
GS-3, and the F16/250 rotors in the
RC-5C PLUS Superspeed Centrifuge.
!