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TissueWave 2 Operator Guide

Shandon TissueWave 2

Microwave Specimen
Processing System

Operator Guide - Issue 1
B35210021 English

Summary of Contents for Shandon TissueWave 2

Page 1: ...1 TissueWave 2 Operator Guide Shandon TissueWave 2 Microwave Specimen Processing System Operator Guide Issue 1 B35210021 English...

Page 2: ...e status of the product If necessary check with Thermo Fisher or your local Thermo Fisher representative This manual may not in whole or in part be copied photocopied reproduced translated or converte...

Page 3: ...D BE AWARE OF THE SAMPLES USED TAKE SUITABLE PRECAUTIONS THIS SYMBOL WARNS YOU THAT SURFACES ARE HOT IF THIS SYMBOL APPEARS ON THE INSTRUMENT ALWAYS REFER TO THIS OPERATOR GUIDE THIS SYMBOL IS USED ON...

Page 4: ...on 3 Installation 4 Programming 5 Troubleshooting 6 Maintenance and Cleaning Appendix A Approved Reagents List Appendix B List of Available Names Appendix C Graph of Power Output Appendix D Power Outp...

Page 5: ...TissueWave 2 Microwave and describes their functions Section 2 Daily Operation This chapter explains the daily operation procedures required to run pre defined protocols Section 3 Installation This ch...

Page 6: ...lectrical shock and the possibility of interference with other electronic devices the unit must always be powered from a grounded receptacle Position the Shandon TissueWave 2 so that it is possible to...

Page 7: ...owavable containers in the Shandon TissueWave 2 Insufficient or improper loads or improper containers metal will cause the magnetron or parts of the microwave interior to overheat When this happens a...

Page 8: ...the chamber flammable materials should not be heated to their boiling point Reagents containing metals eg Zinc Formalin ot Mercuric Chloride need to be completely cleaned off of all internal component...

Page 9: ...overed with fluid during all processing and staining procedures Processing and staining fluids will disperse the heat generated by the microwaves in an even manner throughout the tissue sample This pr...

Page 10: ...The Shandon TissueWave 2 as supplied complies with EN 61010 1 2001 However chemicals introduce potential hazards and Thermo has adopted the following position with regard to the subject of heated surf...

Page 11: ...gents This product is required to comply with the European Union s Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific h...

Page 12: ...strument will free up technical time for more complex procedures The Shandon TissueWave 2 will deliver uniformly excellent results in a fraction of the time Laboratory safety is always an important co...

Page 13: ...EPLACE ONLYWITH SAMETYPE AND RATING EAST GRAN BY CONN ECTIC UT USA ENERGY BEAM SCIENCES INC V A Hz FREQUE NCY2450MHz MANUF ACTURE D MODEL SERIAL C US Power Switch Fuses Magnatron Cooling Outlet Power...

Page 14: ...4 TissueWave 2 Operator Guide Temperature Probe Vacuum Tube Exhaust Port Air Agitator Vacuum Chamber Agitator Valve Temperature Probe Vacuum Tube Exhaust Port Air Agitator Vacuum Chamber Agitator Valv...

Page 15: ...eration The Keypad is used to perform various actions and to interact with the LCD Display The Keys on the Keypad have the following functions Run Stop Quit Enter Cursor Left Right Cursor Up Down Incr...

Page 16: ...TissueWave 2 Operator Guide Interior Light On Off Agitation On Off Function Keys The Function Keys will perform Screen Specific Functions which are noted on the LCD Display adjacent to the Function Ke...

Page 17: ...ew other symbols used on this instrument The meanings of these symbols are as follows Additional Symbols Used On the Instrument Additional Symbols Used On the Instrument Power On Off Switch Serial Por...

Page 18: ...uate air flow established through the microwave chamber This prevents the accumulation of dangerous and toxic fumes The vent system on the Shandon TissueWave 2 is designed to be fed to a Powered Exhau...

Page 19: ...into the Accumulator Canister and collected at the bottom Paraffin will solidify and remain there until re heated for removal The accumulator s collection capacity before vacuum operation is affected...

Page 20: ...processing fluid within the container and at mid depth in the fluid This will produce the most reliable and stable reading of the fluid temperature The Temperature Probe is made from stainless steel a...

Page 21: ...However the operator may override this setting with the Air Agitator Key The Air Agitator Outlet Tube may be removed from the bushing and placed separately in the container for agitation The volume of...

Page 22: ...xplains the routine operating procedures for processing with the Shandon TissueWave 2 The procedures explained are Loading the Shandon TissueWave 2 Internal Connections Running a Protocol Running a Ma...

Page 23: ...sket halves Place the two halves together as shown and insert into the Vacuum Chamber Base Fill to the required level with Processing Fluid Insufficient fluid levels may cause the probe to overheat an...

Page 24: ...2 Operator Guide Insert the Vacuum Chamber into the Shandon TissueWave 2 TISSUEWAVE 2 SHANDON 15 20 10 25 5 30 0 SMC inHg TISSUEWAVE 2 SHANDON TISSUEWAVE 2 SHANDON 15 20 10 25 5 30 0 SMC inHg TISSUEW...

Page 25: ...rature Probe should be at about the Half Way Point of the Depth of Fluid and not in contact with any solid material Securely fit the Vacuum Tube to the Central Connector on the Vacuum Chamber Lid If t...

Page 26: ...Processing Without Vacuum Mount Temperature Probe so that the tip is at about the Half Way Point of the Depth of Fluid and as close to the centre as possible and not in contact with any solid materia...

Page 27: ...he option to proceed to the Next Step Once all steps are complete the Welcome screen will appear Step Runs COMPLETE NEXT START SELECT SELECT START START THIS SCREEN CHANGES TO DISPLAY THE UP COMING ST...

Page 28: ...art Function Key Perform the on screen checks Press the Start Function Key The Run screen will appear and the process will begin START START Use the and keys to alter variables Press and to move the p...

Page 29: ...the available settings until the word Power appears Press the Start Function Key Perform the on screen checks Press the Start Function Key The Run screen will appear and the process will begin START...

Page 30: ...Editing Steps set the Timer Mode to Power for all the steps in a Protocol Run the Protocol as described in Running a Protocol Using the method described in Chapter 4 Editing Steps set the Timer Mode...

Page 31: ...inute test method System Fusing 2 x 15A Slow Blow Temperature Readout Refresh Rate Once per second Temperature Readout Accuracy Temperature Error 0 5 C Power Control Accuracy Power Control Error 1 Air...

Page 32: ...se should be kept to less than 8 feet 2 4 m The Shandon TissueWave draws cooling air through a Filter located on the right hand front bottom surface of the unit and exhausts it through a port on the b...

Page 33: ...a particular Temperature Profile The Temperature Profile can be further defined using the Timer Mode field The Timer Mode options are Time Used to define the Procedure Time from the time when the Sol...

Page 34: ...required at any given time The Shandon TissueWave 2 uses Pulsating Power to control Temperature and Output Power This allows the Shandon TissueWave 2 to provide accurate temperature control and a smo...

Page 35: ...Key Insert the required steps and edit them as required see Add Step and Edit Step Flow Charts SELECT SELECT From the Protocol List use the Up and Down Arrow Keys to highlight New Protocol Press the...

Page 36: ...light the Protocol Name required from the available options Press the Select Function Key to complete SELECT SELECT SELECT From the Protocol List use the Up and Down Arrow Keys to highlight the protoc...

Page 37: ...required step name Press Enter to confirm The step can then be edited as required see Edit Step Flow Chart SELECT SELECT BACK From the Protocol List use the Up and Down Arrow Keys to highlight the Pro...

Page 38: ...etween variables and to alter their values Press the Back Function Key to move the cursor back to the Step Selection position Cursor Moves to Step Selection SELECT SELECT Use the and keys to alter var...

Page 39: ...d Press the Delete Function Key Ensure the correct Step has been selected Press the Yes Function Key NO SELECT SELECT DELETE YES From the Protocol List use the Up and Down Arrow Keys to highlight the...

Page 40: ...om the available options Press the Select Function Key Check that the correct Protocol has been selected Press the Yes Function Key SELECT SELECT NO YES From the Protocol List use the Up and Down Arro...

Page 41: ...and Down Arrow Keys to highlight the Protocol to be renamed Press the Select Function Key Use the Up and Down Arrow Keys to select Rename Protocol from the available options Press the Select Function...

Page 42: ...ng system Check that the hose is properly attached Turn power off and then back on If problems persist call ThermoFisher Scientific Technical Support This is a fatal error Turn power off and then back...

Page 43: ...st call ThermoFisher Scientific Technical Support Check probe for damage If problems persist call ThermoFisher Scientific Technical Support Measured temperature exceeds 120 C Measured temperaure is le...

Page 44: ...Check that the Vacuum Gauge is working correctly Check that the seal between the Vacuum Chamber and its Lid is clear of debris If problems persist call ThermoFisher Scientific Technical Support Check...

Page 45: ...situation can arise if The Temperature Probe is not placed in the reagent being heated The reagent does not absorb the microwave energy sufficiently to reach the Set Point Temperature The Watchdog Ti...

Page 46: ...onnel will Invalidate the Warranty Keep the Air Filter clean The Air Filter is located under the instrument directly below the Control Panel Wash the Air Filter with Detergent and Water and Dry thorou...

Page 47: ...ety Directives for decontamination To avoid any misunderstanding we request that all instruments or parts returned to us must be accompanied by a certificate stating the following We certify that this...

Page 48: ...If the use of a reagent not on this list is required contact your Thermo Fisher agent for advice The Shandon TissueWave 2 should not be used to heat any of the following Potentially explosive substanc...

Page 49: ...Paraffin Wax Fixatives Formalin 10 Ensure proper ventilation when using Formalin based reagents Zinc Formalin Glyo Fixx Epitope Sodium Citrate Buffer 0 1 mol l Retreival PBS Phosphate Buffered Saline...

Page 50: ...RDANT 15 FUCHSIN 55 FORMALIN 95 MUCICARMINE 16 BOUIN S 56 UNMASK 96 PERIODIC SCHIFF 17 FE HEMATOXYLIN 57 BLOOD 97 SAFRANIN 18 STEP 1 58 BLOODY 98 SILVER 19 STEP 2 59 BONE 1 99 SPORE 20 STEP 3 60 BONE...

Page 51: ...g the Power Process Operation Method should be used The following graph can be used to identify the Power Watts Output for a given Duty Cycle Notes The Power Output shown in the graph is a theoretical...

Page 52: ...t provides an approximate qualitative measure of Microwave Output Performance During this two minute test the unit will monitor the percentage of power output and the wattage output Influences on the...

Page 53: ...owave Chamber Place the Temperature Probe centrally in the water Press the Start Function Key The TissueWave 2 will run for 2 minutes at 100 Power Output The Wattage Output and Temperature will be mon...

Page 54: ...ner B1002517 Operator Guide B35210021 Temperature Probe B35240003 Vacuum Chamber B35210001 Microwavable Plastic Coplin Staining Jar 194 Microwavable Plastic Spill Free Slide Jar each 1001362 Microwava...

Page 55: ...your purchase in peak condition for many years to come Warranty provisions necessarily vary to comply with differences in national and regional legislation Specific details can be found in the deliver...

Page 56: ...nostics Directive Low Voltage Directive 98 79 EC 2006 95 EC This product complies with the following International Standards EMC Safety Issued by K Waldron Quality Manager Thermo Fisher Scientific Ana...

Page 57: ...dth 13 5 34 3 cm Chamber Depth 15 5 39 4 cm Chamber Height 9 5 24 1 cm Mass 78 lbs 35 5 kg Electrical Voltage 120 V 240 V Current 14 A 120 V 7 3 A 240 V Frequency 50 60 Hz Maximum supply voltage fluct...

Page 58: ...eral Indoor Use Only Operating Temperature 5 C to 40 C Storage Temperature 25 C to 55 C 70 C for short exposure Humidity Maximum 80 RH to 31 C decreasing linearly to 50 RH at 40 C Altitude Up to 2000...

Page 59: ...Air Agitator Key 16 21 F Air Filter 46 Fluid Ports 13 31 Aluminium Outlet Hose 13 18 32 Function Keys 16 27 Audible Alert 42 Fuses 13 31 C I Chamber Dimensions 31 Improper Load 7 Combustible Fluids 9...

Page 60: ...rformance 51 Sponge Biopsy Pads 9 Output Power 31 Step Complete 27 P T Paraffin Wax 10 Temperature Power Control 33 Control 33 51 Probe 8 14 18 20 25 45 Control Accuracy 31 Profile 33 Output Test 52 R...

Page 61: ...8 19 Chamber 9 18 23 Gauge 13 26 Line 9 14 Ports 17 19 25 Pump 19 31 Release Knob 10 13 Ventilation System 12 18 26 31 Interlock 18 Volatile Materials 8 10 W Warning Screen 42 44 Watchdog Timer 45 Wat...

Page 62: ...62 TissueWave 2 Operator Guide...

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