Running a protocol
22
Thermo Scientific™ Multidrop™ Pico 8 Digital Dispense Operating Manual
Thermo Fisher Scientific
Continue filling reservoirs until all the dispensehead locations have been
filled with the requested fluids.
If the Multidrop Pico dispenser was waiting for a reservoir to be filled
with fluid, when you click Filled, dispensing of that fluid will proceed.
Note
The Load Volume quantity of the Load Fluid prompt will always
be greater than the amount of fluid requested to be dispensed. The
volume to be dispensed for each fluid is labeled as the Total Volume in
the right column of Fluid list on the Main Operating Window. The
difference between the dispense volume and the load volume is to
account for the dead volume, the fluid which is retained in the cassette
after dispensing. The dead volume is dependent upon the dispense
volume and fluid class, with greater dispense volumes and certain classes
of fluids having greater dead volumes. The minimum load volume
ensures proper priming of the cassettes (see
Appendix E – Dispense
Specifications in the Multidrop Pico dispenser Software User Guide).
If you are loading the cassette and click the gray icon to the right of
the cassette graphic, this changes the status of the current fill
dispensehead and all remaining dispenseheads on the cassette to
‘locked’. You can continue to fill the remaining dispenseheads, but no
fluid will be dispensed from the locked dispenseheads until the red icon
is clicked to change the status to ‘unlock.’
The load volume, which is included in reports, can be modified at the
fluid prompt to match the actual volume to be loaded. Load volumes
can only be increased from the required amount.
To modify load volume, move the cursor over the volume on the s and
click when highlighted. A popup box allows the dispensehead volume
to be modified.
Locking
dispensehead
cassettes
Modifying load
volume
