Thermo Scientific KingFisher Flex, User Manual

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Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 89
Glossary
heating block There are four different kinds
of interchangeable heating blocks
available, that is, for KingFisher 24 deep
well plates, Microtiter deep well 96
plates, KingFisher 96 plates, and PCR
plates (
Figure 4–49
).
KingFisher Flex head There are four kinds
of interchangeable KingFisher Flex heads
available, for KingFisher 24 deep well
plates, Microtiter deep well 96 plates,
KingFisher 96 plates, and PCR plates.
The KingFisher Flex heads all have
corresponding disposable plastic tip
combs.
magnetic bead (magnetic particle) The
magnetic particles attach to the magnetic
rods
(
Figure 2–7
and
Figure 2–8
) that are
protected by a disposable tip comb. The
magnetic particles enable the purification
of a variety of target molecules.
magnetic rod The rods which are magnetic
and collect magnetic particles (
Figure 4–
44
). The rods do not collect the particles
on their own; the magnetic rods must
always be protected by a tip comb.
plate The disposable plates (1
–8) where all
the reagents and samples are located and
where the processing takes place. Four
different types of 24 and 96-well plates
(
Figure 4–46
) that can be used are as
follows:

KingFisher 24 deep well plate
(200 µl
– 5 mL*)

Microtiter deep well 96 plate
(50
–1000 µl*)

KingFisher 96 plate (20
–200 µl*)

PCR plate (20
–100 µl*), skirted
* = recommended filling volume
shield plate When the turntable moves, the
shield plate moves over the plate
underneath
(
Figure 4–45
) forming a protective cover.
tip comb Protects the magnetic rods (24 or
96 in a frame). A disposable tip comb
always has to be placed onto the
microplate prior to processing. There are
four different kinds of disposable tip
combs available:

for KingFisher 24 deep well plates

for Microtiter deep well 96 plates

for KingFisher 96 plates

for PCR plates
turntable A turntable with eight plate
stations that can be detached for cleaning
purposes
(
Figure 2–2
).