Thermo Scientific KingFisher Flex, User Manual

Page: 58 / 94

Routine Operation
Changing the heating block
58 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific
Note The sliding door can be left open or the transparent lid can be absent
if desired. This action does not break the run.
s
7. After the run, remove the plate(s) according to the protocol request.
Confirm each plate removal by pressing
START
.
8. Press
STOP
after completing the run.
For startup, refer to
“Switching on” on page 40.
Figure 4
–46.
KingFisher Flex plates
Caution Do not use other plates than the ones listed in Table 4
–4. Other
plates may not be compatible with the KingFisher Flex heating blocks. They
may also cause unexpected problems, such as cross-contamination due to
the divergent well volume and bottom height of the plate.
s
The KingFisher Flex is compatible with KingFisher 24 deep well plates,
Microtiter deep well 96 plates, KingFisher 96 plates, and PCR plates
(Figure 4
–46 and Table 4–4). Specially designed magnetic rods
(KingFisher Flex heads) and tip combs that protect the magnets during the
process are available for different plate types and applications.
Table 4
–4.
Processing volumes vs. plate types and magnetic heads
Head / Plate KingFisher 24
deep well plate
Microtiter deep
well 96 plate
KingFisher 96
plate
PCR plate,
skirted
KingFisher Flex head for
KF 24 deep well plate
200
–5000 µl – – –
KingFisher Flex head for
Microtiter deep well 96 plate
–
50
–1000 µl
50
–150 µl –
KingFisher Flex head for KF 96 plate
– –
20
–200 µl –
KingFisher Flex head for PCR plate
– –
20
–200 µl
20
–100 µl
Changing the heating block is controlled by the software. To change the
heating block, choose the Change_heatblock protocol by using the up and
down cursor keys. Then press
START
. The protocol requests you to remove
the magnets and in the end to insert them. The heating block will thus rise.
Remove the heating block and then replace it
(
Figure 4–47
and
Figure 4–48
). Insert the heating block by first setting
it and then pressing it hard into place. The heating block will snap into
Changing the
heating block