Thermo Scientific HistoStar Operator'S Manual Download Page 16

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HistoStar Operator Guide A81010100 Issue 2

Compatibility

The HistoStar is designed to be compatible with most tissue cassettes and moulds, including Super Mega

Cassettes and SecureSette.
It is also designed to be compatible with most brands of histology grade paraffin wax - see Appendix D -

Approved Reagent List

Summary of Contents for HistoStar

Page 1: ...Thermo Scientific HistoStar Operator Guide A81010100 Issue 2 ...

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Page 3: ...isher Scientific Contact Addresses Anatomical Pathology Int Tudor Road Manor Park Runcorn WA7 1TA UK Tel 44 0 1928 534 050 Fax 44 0 1928 534 049 Web www thermo com pathology Anatomical Pathology USA 4481 Campus Drive Kalamazoo MI 49008 USA Tel 1 800 522 7270 Fax 1 269 372 2674 Web www thermo com pathology Standards The Thermo Scientific HistoStar meets the following CE Mark requirements In Vitro D...

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Page 5: ...is hot If this symbol appears on the instrument or in the documentation always refer to the operator guide Take suitable precautions This symbol indicates that a surface is cold If this symbol appears in the documentation always refer to the operator guide Take suitable precautions This symbol is used on the equipment or in a document to indicate that harmful chemicals are used Refer to the Materi...

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Page 7: ...mmer 24 Switching Off HistoStar 25 Chapter 3 Setup 27 Unpacking HistoStar 27 Connections 34 Setup and Location 37 Setting Workflow Direction 40 Filling with Wax 42 Dispense Lever Adjustment and Wax Flow Control 44 System Settings 47 Time and Date 49 Temperature Units 51 Temperature Set Points 52 Timer Settings 55 Display and Audio 58 Instrument Lighting 60 Forceps Block Power Settings 65 Language ...

Page 8: ...stem Check 89 Chapter 5 Cleaning and Maintenance 91 Safety 91 General Instrument Cleaning 92 Daily Tasks 93 Weekly Cleaning 94 Fuse Replacement 95 Emptying the Waste Wax Drawer 97 Using the Wax Draining Assembly 99 Draining the Tissue Storage Tank 103 Draining the Wax Tank 106 Appendices 111 Appendix A Spares and Accessories 111 Appendix B Packing Instructions Removal from Use and Disposal 113 App...

Page 9: ...upt the Mains supply at the source by removing the plug from the socket If the equipment is used in a manner not specified by Thermo Fisher Scientific the protection offered by the equipment may be impaired Make sure that there is at least 100mm 4in clearance around any fan inlets on the instrument In compliance with statutory requirements all our equipment is designed to accepted standards of saf...

Page 10: ... using The operator has carried out any legally required assessment of chemicals used and is using good laboratory practice Some chemicals which may be used during operation are flammable do not use sources of ignition in the vicinity of the instrument when it is loaded with reagents Harmful chemical vapours such as xylene and toluene may be emitted during the normal operation of some instruments ...

Page 11: ... in peak condition for many years to come Warranty provisions necessarily vary to comply with differences in national and regional legislation Specific details can be found in the delivery documentation or from your dealer or representative Please note that your warranty may be invalidated if This instrument is modified in any way Accessories and reagents which have not been approved by Thermo Fis...

Page 12: ...HistoStar Operator Guide A81010100 Issue 2 4 Product Return Safety Declaration ...

Page 13: ... information with regards to interfacing with the system Chapter 2 Basic Operation This chapter explains how to use the HistoStar on a day to day basis Chapter 3 Setup This chapter explains the procedures used to set up the HistoStar The information contained in this chapter also explains how users can set their own preferences to help HistoStar to fit into their workflow more efficiently Chapter ...

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Page 15: ...ent a wax reservoir a tissue storage tank a wax dispenser and a Para Trimmer HistoStar can be used with either heated or un heated forceps The temperature of various elements can be set using the built in touchscreen user interface Fixed LED lighting is incorporated into the unit in order to illuminate the main working areas The Cold Module consists of a temperature regulated cold plate which can ...

Page 16: ...lity The HistoStar is designed to be compatible with most tissue cassettes and moulds including Super Mega Cassettes and SecureSette It is also designed to be compatible with most brands of histology grade paraffin wax see Appendix D Approved Reagent List ...

Page 17: ...Front View 1 Wax Tank Cover 2 Base Mould Storage Compartment Serial Number Label is inside 3 Tissue Storage Tank 4 Cassette Lid Storage Dish 5 Waste Wax Drawer 6 Hot Spot 7 Cold Spot 8 Dispense Lever 9 Wax Dispense Flow Control 10 Forceps Block Removable Cover 11 Para Trimmer 12 Touchscreen ...

Page 18: ...HistoStar Operator Guide A81010100 Issue 2 10 Heated Module Rear View 13 Footswitch Connector 14 Cold Plate Timer Connector 15 Fuse Drawer 16 Power Switch 17 Mains Power Connector 18 Rating Plate ...

Page 19: ...hapter 1 Introducing HistoStar 11 Heated Module Underside View 19 Dispense Lever Position Adjustment Knob 20 Heated Forceps Connector 21 Tissue Storage Tank Drain Valve Lever 22 Tissue Storage Tank Drain ...

Page 20: ...00 Issue 2 12 Cold Module Cold Module Rear View 23 Cold Plate 24 Timer Control Switch 25 Cold Plate Timer Connector 26 Fuse Drawer 27 Power Switch 28 Mains Power Connector 29 Rating Plate 30 Serial Number Label 31 Refrigeration Label ...

Page 21: ...erences and timer functions Note Avoid using sharp or pointed objects to press buttons on the touchscreen It is recommended that a clean finger is used to operate the touchscreen However if you require a stylus the eraser tipped end of a pencil or similar is suitable The touchscreen is used to alter all the user preferences and setup features see System Settings ...

Page 22: ...ious screen and Left and Right Used to scroll through lists and Up and Down Used to increase or decrease values and scroll through lists Sleep Used to enter Sleep Mode Timer Off Used to disable the timer function Note There are many text buttons throughout the user interface which perform specific actions depending on the screen These are explained in the relevant sections of this document ...

Page 23: ... Frequency 50 60 Hz Power 700VA Fuses 2 x T10AH Environmental Specification Temperature Operating Limits 5 C to 40 C 41 F to 104 F Temperature Recommended Operation 17 C to 27 C 63 F to 80 F Note Performance may deteriorate when operated outside of this temperature range Temperature Transport Storage 25 C to 55 C 13 F to 131 F 70 C 158 F for short exposure Relative Humidity Max 80 RH up to 31 C De...

Page 24: ... x T10AH Environmental Specification Temperature Operating Limits 5 C to 40 C 41 F to 104 F Temperature Recommended Operation 17 C to 27 C 63 F to 80 F Note Performance may deteriorate when operated outside of this temperature range Temperature Transport Storage 25 C to 55 C 13 F to 131 F 70 C 158 F for short exposure Relative Humidity Max 80 RH up to 31 C Decreasing linearly to 50 RH at 40 C Alti...

Page 25: ...ck the temperature of the wax in the wax tank using a calibrated thermometer Compare the measured value with the reading on the touchscreen display The two readings should be within 2 0 C of each other at any setting If this is not the case contact your local Thermo Fisher Scientific representative Do not use wax with a melting point in excess of 70 C 167 F as it may not melt The HistoStar is seal...

Page 26: ...first time Ensure all power switches are in the OFF position and that all the HistoStar modules are connected to a suitably rated power supply Ensure the cold plate timer connector on the Embedding Module is connected to the matching connector on the Cold Module Ensure there is wax in the wax tank either molten or in pellet form Damage to the unit can occur if HistoStar is run for extended periods...

Page 27: ... the following screen will be displayed on the touchscreen display To exit Sleep Mode touch the ON button at the bottom of the screen Note If HistoStar has been in Sleep Mode for an extended period of time it may be necessary to wait a while whilst the Cold Module achieves its operating temperature Cold Module Stand Alone Power Up If the Cold Module has been moved recently allow the unit to stand ...

Page 28: ...hat melted wax covers all of the cassettes Remove a cassette from the tissue storage tank and remove the lid the lid can be stored in the cassette lid storage dish Note When storing cassettes in the tissue storage tank it is recommended that they be kept in an organizer basket Place the cassette on one of the hot areas in front of the tissue storage tank Select a suitable clean base mould from the...

Page 29: ...pecimens may result in cross contamination of tissue If tissue debris is transferred to the work surfaces ensure it is cleaned off before proceeding If recovering a dropped specimen from the work area or drainage route take care to ensure the specimen is not damaged Ensure that the tips of forceps are kept clean to avoid cross contamination of samples Move the base mould onto the cold spot and wai...

Page 30: ...to the hot spot and place the cassette onto the base mould Fill the cassette with wax Note Use the wax flow control knob to adjust the wax flow rate to a suitable setting Incorrect flow settings can cause voids to form in the wax and or difficulties when orienting specimens ...

Page 31: ...frost build up will vary with the humidity in the laboratory Frost build up will not normally interfere with cooling but if excessive frost develops turn the Cold Module off for a short period until defrosting occurs Remove any water from the cold plate surface using a dry cloth An hour on the cold plate should be more than adequate to ensure that the wax is fully set Prolonged chilling can cause ...

Page 32: ...te across the serrated face of the Para Trimmer to scrape off the excess wax The surface of the Para Trimmer is hot and care should be taken to avoid skin contact during use The Para Trimmer edges are sharp Incorrect use can cause injury and or specimen damage Repeat the above procedure for all edges until the cassette is clean Notes The Para Trimmer is safe for cleaning the printed surface of a c...

Page 33: ...timer control entering Sleep Mode will turn it off The cold spot and hot spots on the embedding module will also be switched off This will not turn off the wax tank the tissue storage tank or the base mould storage area Switching Off HistoStar Completely To switch HistoStar OFF completely Remove any tissue samples from the tissue storage tank Switch the power switch on the back of each module to t...

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Page 35: ... Fisher Scientific representative Note If the Cold Module has been purchased as a stand alone unit the outer packaging will differ from that shown however the sections which refer to the specific unpacking of the Cold Module will still apply Unpacking the Modules To unpack the HistoStar modules Remove the lid and the outer sleeve and shown ...

Page 36: ... 28 Lift off the accessories box and set aside for unpacking later Lift out the cold module using the handles in the cardboard packing The cold module weighs approximately 20kg with uneven weight distribution seek help when lifting if necessary ...

Page 37: ... that the unit is carried in the manner shown below failure to do so may result in personal injury and or damage to the unit Fold down the side panels of the packing and manoeuvre the cold module out of the foam pieces as shown ...

Page 38: ...ing module using the handles in the cardboard packing The embedding module weighs approximately 25kg seek help when lifting if necessary Ensure that the unit is carried in the manner shown below failure to do so may result in personal injury and or damage to the unit ...

Page 39: ...Chapter 3 Setup 31 Fold down the side panels of the packing and manoeuvre the embedding module out of the foam pieces as shown ...

Page 40: ...010100 Issue 2 32 Assembling HistoStar To assemble the various components Place the Tissue Storage Tray Forceps Block Cover and Waste Tray in the locations shown Place the Tissue Storage Cover over the Tissue Storage Tank as shown ...

Page 41: ...r backwards and slide the raised edge at the back underneath the bottom edge of the Base Mould Storage Compartment This allows the raised edge to act as a drawer stop preventing the cover from being accidentally pulled too far forward ...

Page 42: ...ated Mains Power Supply see System Specification Insert the end of the power lead into the power inlet connector on each module Connect the loose end of each lead to the Mains Supply Switch the Mains Supply ON Timer Control Connection To connect the Cold Module to the Embedding Module for Timer control Insert one end of the timer control lead into the cold plate timer connector on the Cold Module ...

Page 43: ...e timer control lead is inserted in the correct orientation as shown Connect the loose end of the timer control lead into the cold plate timer connector on the Embedding Module Switch the timer control switch to the TIMER position ...

Page 44: ...h Insert the open end of the footswitch tubing into the footswitch connector port on the Embedding Module Ensure the tubing is fully inserted otherwise the footswitch may not operate correctly Route the footswitch bellows under or behind the unit and place on the floor in front of the dispense station ...

Page 45: ...ar module should be located with at least 100mm 4in of clearance at the rear as shown The air vents on the back of the HistoStar modules should be unobstructed at all times The air vents below the cold spot on the Embedding Module should be unobstructed at all times It is advisable to ensure that there is also at least 75mm 3in of clearance at the sides of the HistoStar ...

Page 46: ...r modules including the weight of the wax and accessories see System Specification The work bench must be level and made of non flammable material Do not position the HistoStar directly under air conditioning or heating vents or near to an open window Do not position the HistoStar in an area where it is likely that the operator could be bumped or knocked during embedding Keep out of direct sunligh...

Page 47: ...le The correct levelling should have the rear of the instrument approximately 1mm higher than the front Once the instrument is levelled correctly tighten the lock nuts to secure the adjustable feet Note The levelling of the HistoStar does not need to be particularly precise and the factory setting should be suitable for most cases ...

Page 48: ...ne how it is arranged in order to suit their workflow and laboratory layout The number of modules can also be varied as required Default Workflow Arrangement The default workflow arrangement for which HistoStar has been optimised is from right to left as shown Note Examples throughout this operator guide assume this layout ...

Page 49: ...n alternative arrangement is shown below It is also possible to create unique workflows by varying the number of each type of module see below for example Note Only one Cold Module can be timer controlled from a single Embedding Module ...

Page 50: ...Operator Guide A81010100 Issue 2 42 Filling with Wax To fill the wax tank Open the wax tank cover to allow access to the wax tank Use a beaker or other suitable container to pour pre molten wax into the wax tank ...

Page 51: ...ended that wax pellets are only added directly to the wax tank when there is still about a quarter of a tank of molten wax still in the tank Wax pellets added directly to the wax tank when it is empty can take up to 6 hours per kilogram to melt Do not overfill Maximum fill level is 20mm below the top of the tank Molten wax is hot and can cause severe burns Close the wax tank cover ...

Page 52: ... reposition the dispense lever and or adjust the flow rate of the wax to suit your specific working habits Adjusting the Dispense Lever Position To adjust the position of the dispense lever Loosen the dispense lever position adjustment knob by approximately 1 full turn as shown Do not remove the dispense lever position adjustment knob ...

Page 53: ...g the dispense lever always ensure that the wax dispense flow control knob is set to the lowest possible setting to avoid accidentally dispensing hot wax whilst moving the mechanism Tighten the dispense lever position adjustment knob to lock the dispense lever mechanism in the new position ...

Page 54: ...nse flow rate turn the wax dispense flow control knob to the required setting Notes Turning the adjustment knob counter clockwise will increase the flow of wax turning the adjustment knob clockwise will reduce the flow of wax Turning the adjustment knob fully clockwise may not cause the wax to stop flowing ...

Page 55: ... and Audio Instrument Lighting Forceps Block Power Settings Language Selection These settings can all be accessed from the Main Menu screen Note The Temperature Set Points and Language Selection settings can be accessed via other screens too Details are provided in the relevant sections To view the Main Menu screen after switching on the HistoStar Wait for the system to load and display the System...

Page 56: ...HistoStar Operator Guide A81010100 Issue 2 48 Press the Menu button to display the Main Menu screen ...

Page 57: ...Chapter 3 Setup 49 Time and Date To set the time and date Press the Timer button on the Main Menu Press the Set Time Date button on the Time Menu ...

Page 58: ...the value to change Use the Up and Down buttons to increase or decrease the highlighted value Press the Return button to exit the screen when finished Note HistoStar does not start to count the time until the Set Time Date screen has been exited HistoStar does not automatically adjust the time to allow for Daylight Saving Time ...

Page 59: ... degrees Celsius C and degrees Fahrenheit F Press the Options button in the Main Menu Press the Units button in the Options Menu the value shown will change to the alternate unit type Pressing the Units button again will change back to the original units Note The default unit is degrees Celsius ...

Page 60: ... Status button in the Main Menu When the Status Menu appears select the item to control Note It is advisable to have the Status Menu displayed during normal operation The Status Manu allows quick access to the settings which are likely to be changed most often It also gives a visual indication of the temperatures around the instrument and will alert the user of any faults which may occur ...

Page 61: ...ge Each of the buttons displays the current temperature of the heated area Pressing any of the four buttons listed above will display the relevant Settings screen Note The appearance of the screens for the heated areas will vary although they will all have the same features as shown below To switch the heater for the selected area OFF Press the Paraffin Tank button The button image will change to ...

Page 62: ...ure all wax is fully molten before use Ensure the temperature settings for all heated areas is appropriate for the type of wax being used Cooling Options Button Pressing the Cooling Options button will display the following screen The two buttons display the current condition of the relevant cold area Pressing either of the buttons will toggle the specific cold zone between ON and OFF Press the Re...

Page 63: ...ff areas of the instrument at given times The timer allows individual programs to be defined for all 7 days of the week There is also the option to disable the timer function on specific days if required For the cold module to be controlled by the timer function the timer control lead must be connected and the timer control switch on the cold module must be set to the Timer position see Connection...

Page 64: ...Program Timer screen will appear Disable Timer To disable the timer function for any given day In the Program Timer screen use the Left and Right buttons to scroll through the days of the week until the required day is displayed Press the Timer Off button The word Disabled will appear below the day ...

Page 65: ... the days of the week until the required day is displayed Press the Edit button The screen will change as shown Use the Left and Right buttons to highlight the value to change Use the Up and Down buttons to increase or decrease the highlighted value Press the Return button to exit the screen when finished Note The minimum increment for timer setting values is 5 minutes ...

Page 66: ...HistoStar Operator Guide A81010100 Issue 2 58 Display and Audio To change the display and audio settings Press the Options button in the Main Menu Press the Display button in the Options Menu ...

Page 67: ...equired contrast value from 1 to 5 5 is the darkest setting and 1 is the lightest The current setting is displayed in the top right hand corner of the screen Audio Settings An audible tone can be set to occur every time the touchscreen is activated To switch the tone on or off In the Display Settings screen press the Touch Beep button to toggle between ON and OFF ...

Page 68: ... during embedding One set of lights is located at the end of the wax dispense arm and are referred to as Hot Spot Lights The other set of lights is located in the instrument casing below the touchscreen display These are referred to as Accessory Lights To access the Light Menu Press the Options button in the Main Menu Press the Light button in the Options Menu ...

Page 69: ...en will appear Adjust Hot Spot Lights Settings To adjust the hot spot lights settings Press the Hot Spot Light button to access the Hot Spot Lights Settings screen Use the Up and Down buttons to increase or decrease the Idle Minutes setting ...

Page 70: ...on for a period of time This period of time is defined by the Idle Minutes setting This setting disables the hot spot lights Once the settings have been configured as required press the Return button to return to the previous screen Hot Spot Lights Power Settings To adjust the hot spot lights power settings In the Hot Spot Lights Settings screen press the Power Options button to access the Hot Spo...

Page 71: ...t and Right buttons to scroll through the activation options below This setting links the accessory lights to the hot spot lights so that they are both on off at the same time This setting switches the accessory lights on permanently This setting disables the accessory lights Once the settings have been configured as required press the Return button to return to the previous screen ...

Page 72: ...cessory lights power settings In the Accessory Lights Settings screen press the Power Options button to access the Accessory Lights Power Settings screen Select a power setting between 1 and 5 where 5 is the brightest and 1 is the most dim Press the Return button to return to the previous screen ...

Page 73: ...Chapter 3 Setup 65 Forceps Block Power Settings To adjust the forceps block power setting Press the Options button in the Main Menu Press the Forceps button in the Options Menu ...

Page 74: ...isplay the Forceps Block Settings screen Select a power setting between 1 and 5 where 5 is the warmest and 1 is the coolest Press the Return button to return to the previous screen The tips of forceps in the forceps block can reach up to 65 C Avoid skin contact Ensure forceps are at an appropriate temperature before use ...

Page 75: ...Chapter 3 Setup 67 Language Selection To change the language which the user interface is displayed in Press the Options button in the Main Menu Press the Language button in the Options Menu ...

Page 76: ...ll appear Use the Up and Down buttons to scroll through the available languages Press the Return button to return to the previous screen Note The Language screen can also be accessed from the Startup screen by pressing the Language button in the bottom left hand corner ...

Page 77: ...ation Switch HistoStar Off using the power switch see Switching Off HistoStar Wait until the touchscreen goes blank and then place a finger on the touchscreen Keeping the finger firmly in contact with the touchscreen switch HistoStar On using the power switch see Switching On HistoStar The following screen should appear Remove the finger from the screen the following screen will appear ...

Page 78: ...oStar Operator Guide A81010100 Issue 2 70 Touch the A button the screen will change to the following Touch the B button to complete the calibration sequence HistoStar will now begin to initialise as normal ...

Page 79: ...ories see Appendix A Spares and Accessories The heated forceps come in 3 different tip sizes and have been designed specifically for use with the HistoStar Only use HistoStar forceps with the HistoStar use of other forceps may cause damage to the instrument and or the forceps Do not use HistoStar forceps with any other instrument including the Histocentre 3 ...

Page 80: ...e electric forceps are connected via the socket at the front of the embedding module There is also a small hook which can be used to guide the wire to the left hand side of the dispense if required Only use connect disconnect the electric forceps when the instrument is switched off ...

Page 81: ...Chapter 3 Setup 73 Once connected the electric forceps should be located in the smaller front holes of the forceps block when not in use ...

Page 82: ...010100 Issue 2 74 Using the Electric Forceps The electric forceps must be switched on before they can be used To switch the electric forceps on Press the Options button in the Main Menu Press the Forceps button in the Options Menu ...

Page 83: ...s Options screen appears Press the Electric Forceps button to toggle the electric forceps On and Off Ensure the electric forceps are at the correct temperature before use The tips of the electric forceps are hot during use only hold the forceps using the insulated grips ...

Page 84: ... the electric forceps switched On and at their working temperature use a dry lint free cloth to wipe any excess wax off the tips Never use xylene to clean the electric forceps Never immerse the electric forceps in any liquid other than molten wax Only immerse the tips of the forceps up to the moulded insulation ...

Page 85: ...not turn on Unit is not powered Switch the embedding module off for 30 seconds and then back on again to reset the control system Check that the Mains Lead is connected correctly see Connections Key touch position is incorrect Touchscreen positional misalignment Recalibrate the touchscreen see Touchscreen Calibration ...

Page 86: ...r clockwise Instrument malfunction Switch the embedding module off for 30 seconds and then back on again to reset the control system Perform Heater System Check Call Thermo Fisher Scientific Service Department Wax will not dispense when using footswitch Disconnected or poorly fitted air tube Check that air tube is fully inserted into footswitch connector port see Connections Ruptured tubing or foo...

Page 87: ... after a suitable length of time Switch off the embedding module Wait for 20 minutes and then switch the embedding module back on Perform Heater System Check Call Thermo Fisher Scientific Service Department Excessive wax drips from the dispenser Debris blocking the valve seal Call Thermo Fisher Scientific Service Department Wax dispenses continuously Dispense lever jammed Check operation of dispen...

Page 88: ...off forceps Power level too low for wax in use Increase the power level of the forcep block see Temperature Set Points Does not heat up Instrument malfunction Switch the embedding module off for 30 seconds and then back on again to reset the control system Perform Heater System Check Call Thermo Fisher Scientific Service Department ...

Page 89: ...re if the set point temperature is not achieved after a suitable length of time Switch off the embedding module Wait for 20 minutes and then switch the embedding module back on Perform Heater System Check Call Thermo Fisher Scientific Service Department Hot spot lights do not switch on Lighting not switched on Switch on the hot spot lights see Instrument Lighting Instrument malfunction Switch the ...

Page 90: ...fter a suitable length of time Switch off the embedding module Wait for 20 minutes and then switch the embedding module back on If problem persists call Thermo Fisher Scientific Service Department Wax surface not melted Close tissue storage cover during warm up as draughts can cause a skin to form on the surface of the wax Tank does not heat up Instrument malfunction Switch the embedding module of...

Page 91: ... 4 Troubleshooting 83 Temperature Sensors Problem Possible Cause Remedy Display shows current temperature as XXX Sensor malfunction Perform Heater System Check Call Thermo Fisher Scientific Service Department ...

Page 92: ...t point temperature to the actual temperature if the set point temperature is not achieved after a suitable length of time Switch off the embedding module Wait for 20 minutes and then switch the embedding module back on If problem persists call Thermo Fisher Scientific Service Department Instrument malfunction Switch the embedding module off for 30 seconds and then back on again to reset the contr...

Page 93: ...he required temperature yet Allow sufficient time for the cold spot to cool Fan air flow blocked Remove blockage from fan inlet directly underneath cold spot Instrument malfunction Switch the embedding module off for 30 seconds and then back on again to reset the control system Call Thermo Fisher Scientific Service Department ...

Page 94: ...lfunction Call Thermo Fisher Scientific Service Department Compressor will not switch on Cold module not powered Check fuses and replace if necessary see Fuse Replacement Check that Mains lead is connected correctly Cold module set for Timer operation but not connected to embedding module Either Connect to embedding module see Connections or Change setting to Compressor On Power not switched on vi...

Page 95: ... or fail to reach working temperature Power is not switched on Ensure electric forceps are switched on see Electric Forceps Ensure temperature setting is correct Faulty or poor connection Ensure the jack is fully inserted into the connection socket Failure of heating element Replace forceps ...

Page 96: ... to use Surface of block is uneven due to unclean mould Ensure the moulds are clean before use Tissue storage tank is too hot Check the melting point of the wax and reduce the temperature setting as required see Temperature Set Points Cross contamination of wax in wax tank mixing of different waxes Drain the wax tank see Draining the Wax Tank Clean the wax tank see General Instrument Cleaning Refi...

Page 97: ...c Service Department for technical support From the Status Menu press the button for the required heater Check that the heater is set to On Check that the current temperature is within 5 C of the set point temperature Note Ensure that a suitable amount of time has been allowed for the heater to reach its set point temperature If the current temperature is displayed as XXX this indicates that there...

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Page 99: ...h room temperature prior to cleaning When dealing with solvents and cleaning chemicals take all suitable precautions to minimize exposure during cleaning Cold plate should be switched off when not in use to prevent excessive frost build up Before using any cleaning or decontamination method except those specified in this document users should check with their Thermo Fisher Scientific representativ...

Page 100: ...d wax from the surfaces Once the wax has been removed use a damp lint free cloth to wipe the surfaces Cleaning the Heated Surfaces To clean the heated surfaces Use a dry lint free cloth to wipe the surfaces clean Pay particular attention to the drainage channels Clean the forceps block holes to ensure that any debris is removed Note A cotton tipped swab is useful for cleaning the drainage channels...

Page 101: ...te wax tray Note In the case of a large spillage on the main deck pay close attention to the level of wax in the waste wax tray and empty as required Wipe the mould storage area to remove any wax build up pay particular attention to the front lip area where the wax should collect Wipe the surface of the cold plate Check the wax level in the wax tank and top up as required ...

Page 102: ... and wipe away any wax Clean the touchscreen Remove waste wax tray and clean around the drain holes and the metal plate that the tray rests on Check for wax build up on the casework and remove if necessary Remove storage drawer in cold module and clean if necessary Depending on usage it may be necessary to drain the tissue storage tank and refill it with fresh wax ...

Page 103: ...nce 95 Fuse Replacement To replace the fuses Ensure HistoStar is switched off and disconnected from the mains power supply Remove the power lead from the mains inlet connector Open the hinged cover to allow access to the fuse drawer ...

Page 104: ... drawer and replace Note Always replace fuses as a pair Ensure the replacement fuses are the correct rating see System Specification Fuses should only be replaced by a competent person To replace the fuse drawer follow the above procedure in reverse If the fuses blow frequently repeatedly disconnect the unit from the mains supply and contact the Thermo Fisher Scientific Service Department ...

Page 105: ...sure that the tissue storage tank is switched on when emptying as this will ensure that the wax remains molten and does not solidify in the bottom of the removable waste tray Completely remove the waste wax tray from the unit Lift out and empty the removable waste tray Take care when removing the waste tray as it may be hot ...

Page 106: ...ons Use a dry paper towel to wipe any molten wax off the waste tray and the metal plate that the drawer rests on in the instrument Use the spatula provided to scrape any dried wax off the metal plate and the drawer moulding Replace the waste tray in the waste wax drawer Replace the waste wax drawer ...

Page 107: ...aining Assembly is used to aid safe and efficient draining of molten wax from the HistoStar The Wax Draining Assembly is made up of several components which must be assembled before use Assembly Completely remove the waste wax tray from the unit Fit the wax draining funnel as shown ...

Page 108: ...that the slides on the edges of the funnel are located on the drawer runners as shown below The HistoStar should be positioned so that the funnel hole overhangs the edge of the worktop allowing the assembly to hang below it Hang the two side pieces on the edges of the wax draining funnel as shown ...

Page 109: ...es as shown Note Ensure the hooks locate in the slots in the base plate Place a suitable leak proof container on the base Notes A 2 5kg Thermo Scientific Histoplast box including the internal plastic bag is ideal Maximum recommended dimensions of the container are 170 x 165 x 210mm W x D x H ...

Page 110: ...HistoStar Operator Guide A81010100 Issue 2 102 The Wax Draining Assembly is now ready to use See also Draining the Tissue Storage Tank Draining the Wax Tank ...

Page 111: ... is molten and the tissue storage tank is switched on Note It is advisable to ensure that the hot spot is switched on when draining as this will ensure that the wax remains molten until it has exited the internal pipework Completely remove the waste wax tray from the unit Fit the wax draining container assembly see Using the Wax Draining Assembly ...

Page 112: ...HistoStar Operator Guide A81010100 Issue 2 104 To drain the wax pull the drain valve lever outwards as shown The wax should drain out of the tissue storage tank and into the wax draining assembly ...

Page 113: ...ete take care when lifting If the tissue storage tank has been overfilled it may be necessary to change the box part way through the draining process Take care to avoid spilling molten wax during draining Wax being drained is hot and can cause severe burns Dispose of drained wax according to local regulations ...

Page 114: ... tank are switched on when draining as this will ensure that the wax remains molten until it has exited the internal pipework It is recommended that the wax tank should be no more than half full before draining If the wax tank is too full manually remove wax from the wax tank by opening the wax tank cover and bailing out the wax using a suitable vessel Completely remove the waste wax tray from the...

Page 115: ...Chapter 5 Cleaning and Maintenance 107 Fit the wax draining assembly see Using the Wax Draining Assembly To drain the wax push the dispense lever towards the back of the instrument as shown ...

Page 116: ...Operator Guide A81010100 Issue 2 108 The wax will drain out of the wax tank and into the wax draining channels and out of the drain hole The wax should drain from the drain hole and into the wax draining assembly ...

Page 117: ... in the drainage channels to drain into the wax draining assembly When removing the wax draining assembly immediately insert the waste wax tray to catch any further residue Take care to avoid spilling molten wax during draining Wax being drained is hot and can cause severe burns Dispose of drained wax according to local regulations ...

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Page 119: ...16629 Footswitch A81010033 Electric heated forceps Red 1mm tip A81010034 Electric heated forceps Yellow 2mm tip A81010035 Electric heated forceps Blue 4mm tip A81000004 Cold module 220 240V 50 60Hz A81000005 Cold module 100 120V 50 60Hz A81000006 Cold module 220 240V 50 60Hz A81010036 Document holder kit China variant not available in ROW Spares Part Number Description A81010037 Fuse kit A81010055...

Page 120: ...st PE 8 x 1kg 8331 Histoplast IM 8 x 1kg 8332 Histoplast LP 8 x 1kg 9810 Fast Flex 10 x 2lb 8334 Type 1 10 x 2lb 8335 Type 3 10 x 2lb 8336 Type 6 10 x 2lb 8337 Type 9 10 x 2lb 8338 Type H 10 x 2lb 8339 Type L 10 x 2lb B1002490 PrcsnCt56 58C 4 x 2 5kg T8330 Ameriplast PE 8 x 1kg T8331 Ameriplast IM 8 x 1kg T8332 Ameriplast LP 8 x 1kg 031 557 Fisher Brand 8 x 2lb ...

Page 121: ... box see Unpacking HistoStar Pack the modules and accessories box by reversing the unpacking instructions see Unpacking HistoStar Use sticky tape to keep the wax tank lid and base mould storage compartment door from opening during transit If returning the unit to Thermo Fisher Scientific ensure that the Product Return Safety Declaration certificate has been completed and is packed with the shippin...

Page 122: ...HistoStar Operator Guide A81010100 Issue 2 114 Appendix C Screen maps ...

Page 123: ...ote Use of unapproved reagents may cause damage to the instrument and or samples Waxes Paraffins Histoplast Histoplast LP Histoplast PE Histoplast IM Fast Flex Type 1 Type 3 Type 6 Type 9 Type H Type L PrcsnCt56 58C Ameriplast LP Ameriplast PE Ameriplast IM Fisher Brand Cleaning Reagents Water 10 Commercial Bleach ...

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Page 125: ... Heated Forceps 9 Mains Power 9 D Daily Tasks 93 Date 49 Depth 15 Disable Timer 55 Dispense Flow Control 9 Dispense Lever 9 20 106 Dispense Lever Position 44 Display Settings 47 58 Drain Hole 106 Drain Valve 9 Drainage 37 Draining Channels 106 E Edit Timer 55 Electrical Safety 1 Electrical Specification 15 Environment 1 Environmental Specification 15 F Fahrenheit Degrees 51 Flow Control Adjustment...

Page 126: ...Directive 1 S Safety Information 1 17 91 Screen Map 114 Service Contracts 1 Settings 47 Audio 58 Date 49 Display 58 Forceps Block Power 65 Language 67 Lights 60 Temperature Set Points 52 Temperature Units 51 Time 49 Timer 55 Touchscreen Calibration 69 Setup 37 Sleep Mode 18 25 Spares 111 Symbols 5 System Menu 47 T Temperature Operating Limits 15 Recommended Operating 15 Set Points 52 Transport 15 ...

Page 127: ...Index 119 Waste Wax Tray 9 103 106 Wax Dispense Flow Control 44 Wax Draining Container 103 106 Wax Tank 42 Cover 8 Draining 106 WEEE Directive 1 Weekly Tasks 94 Weight 15 Width 15 Workflow 40 ...

Page 128: ...120 ...

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