Chapter 4 – Using the Instrument
2.
Set up method parameters.
Parameter
Function
Measure Standards
Use Enter to toggle between Yes and No.
•
Yes:
Enter the Ids of the standards and
concentrations into the Standards Table. After
pressing the Calibrate function key, the user
will be prompted to measure each standard.
•
No:
Highlight the first standard ID in the
Standard Table and press Enter.
The Library page will open showing available
.FXD results files. Highlight the required
Fixed multiwavelength result file and press the
Load File function key. The method
parameters will be set to those used to measure
the first standard and cannot be changed.
All subsequent standards must have been
measured using exactly the same method. Each
file must contain data for a single sample. Use
Clear Results between Fixed measurements.
Standards
Transfers to the MCA standards page.
•
When Measure Standards = Yes, enter an ID
and concentration for each standard.
•
When Measure Standards = No, load
Standards as described above and enter a
concentration for each standard.
Wavelengths
•
When Measure Standards = Yes, enter the
wavelengths at which standards and samples
will be measured. Wavelengths may be entered
from a scan.
•
When Measure Standards = No, the
wavelengths used are defined by the standard
method.
Note
At least one wavelength must be entered for each standard. Choose
wavelengths at which there are significant differences between standards.
3.
Clear the beams or insert suitable blank(s) and close the lid of the
sample compartment.
4.
Press Zero.
50
Evolution 300 and Evolution 600 User’s Guide
Thermo Electron Corporation
