Chapter 4 – Using the Instrument
The method will be executed and the absorbance(s) of the sample(s)
will be plotted on the Rate Graph.
When the Pause function key is selected, data collection will cease but
the clock will continue to run. When the Pause function key is selected
again, data collection will resume. There will be a gap in the Kinetics
Graph for the duration of each pause. In Parallel Rate, the pause will
not take effect until the current cycle of measurements has been
completed.
8.
Press the Manipulate function key to access the Manipulate menu.
9.
Rescale, smooth or change the absorbance display, if required.
Select Original to return to the unmanipulated data.
10.
Set up the rate calculations.
Section allows up to 5 markers to be placed on the graph defining up to
4 contiguous time intervals over which the rate statistics will be
calculated.
When the markers have been placed, press the function key to exit from
the Section Mode.
11.
Press the View Results function key to see the results of the rate
calculations performed using Section.
Function keys on the Results page enable you to save the results to the
instrument Library or to disk.
12.
Press the Save Results function key to save the results to the
instrument Library or to disk.
48
Evolution 300 and Evolution 600 User’s Guide
Thermo Electron Corporation
