4
CyFlow
™
Space | Operating Manual | March 2021
Table of figures
Figure 1:
Cells are separated in a suspension and stained with fluorescent markers ..... 9
Figure 2:
Light from the laser(s) excites cell-bound fluorophores and is scatted by the
cells .................................................................................................................. 9
Figure 3:
Fluorescence signals are displayed and analysed in histogram and/or dot plot
diagrams ......................................................................................................... 10
Figure 4:
True Volumetric Absolute Counting by the Electrode Principle ...................... 18
Figure 5:
Exemplary demonstration of the optical bench of a CyFlow™ Space with 2
lasers and 8 parameters (left) and 3 lasers and 8 parameters (right). Other
laser light sources and parameter allocations are possible. D = detector (photo
multiplier) ........................................................................................................ 22
Figure 6:
Optical geometry at the laser interrogation (schematics) ............................... 23
Figure 7:
Multiparameter instrument settings box ......................................................... 24
Figure 8:
The Parameter Setup Dialog Box ................................................................... 24
Figure 9:
Sample Transporting System of the CyFlow™ Space. .................................. 28
Figure 10:
Recommended placement of CyFlow™ Space components ......................... 32
Figure 11:
Instrument Setup and periphery (CyFlow™ Space shown from the rear) ...... 33
Figure 12:
Computer connection on the CyFlow™ Space rear ....................................... 34
Figure 13:
Power connection and light source switches on the left side of the CyFlow™
Space. ............................................................................................................ 35
Figure 14:
Tube connections for sheath and waste containers and cable connections for
the fluid level sensors on the right side of the CyFlow™ Space. .................... 36
Figure 15:
Flow cuvette with sample, sheath fluid and waste connections. .................... 37
Figure 16:
Warning laser radiation .................................................................................. 40
Figure 17:
Additional explanation .................................................................................... 40
Summary of Contents for CyFlow Space
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