CyFlow
™
Space | Operating Manual | March 2021
27
The gain value of a parameter can be selected by clicking into the corresponding
gain value field in the instrument settings box, please see chapter 9 Instrument
Settings, Figure 7. By using the Right (Left) buttons gain values are increased
(decreased).
In case linear amplification is used, peaks are expanded to the right (compressed
to the left) when increasing (decreasing) the gain (see example).
In case logarithmic amplification is used, peaks are moved to the right (left) when increasing
(decreasing) the gain.
Use the gain to move the peaks of interest to a suited position in the histograms.
Start with low gain values around 250. When increasing the gain, make sure the most
intense signals still appear in the histogram and are not moved out of scale.
Increasing (decreasing) the gain by 50 approximately causes an increase (decrease) of
amplification by a factor of two. Gain values between 250 and 600 are a good start for
adjustment.
For each parameter, you can choose between linear or logarithmic amplification. In
logarithmic amplification mode, the signal range can be set to 3 (log3) or 4 decades (log4),
corresponding to a range of 1...1000 or 0.1...1000. Generally, a logarithmic amplification is
advisable if particles with a broad range of intensities above 1:10 are to be analysed.
Click the little arrow, select one of the following
lin
(linear)
log3
(logarithmic, 3 decade range)
log4
(logarithmic, 4 decade range)
and click it.
9.1.3 Sample
Speed
Adjustment of sample speed
By means of the speed value, the sample speed (in terms of µl/s) is set.
The speed value can be increased (decreased) by clicking into the
corresponding field in the instrument settings box and using the Right (Left) buttons.
The count rate increases with elevated speed values. If the speed is increased too much,
peaks in the histograms may become wider as a result of decreasing accuracy.
If the speed is too low, particle sedimentation effects can influence a counting result.
Summary of Contents for CyFlow Space
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