20
CyFlow
™
Space | Operating Manual | March 2021
NOTICE
If in Acquisition Setup/Acquisition Control, the function Clear Histograms
on Count Start is enabled all data acquired during RUN phase prior to the
COUNT phase will be deleted. The final fcs data file will contain only the
data acquired during COUNT phase of 200 µl sample. Analysing the data
with 3
rd
-party fcs software will allow the calculation of concentrations. To
achieve numbers per ml multiply the count with the factor 5 (count in 0.2
ml x 5 = count / ml).
If the function, Clear Histograms on Count Start is disabled FloMax
®
will
label the data set acquired during COUNT phase but will maintain all data
of RUN and COUNT phase. In FloMax
®
Cell concentration will be
calculated on basis of the data label. In 3
rd
party software a calculation of
concentration is not possible as a read out of the data label is not
possible.
CAUTION
When analysing data without TVAC make sure Clear Histograms on
Count Start is disabled. Otherwise data are lost when entering COUNT
phase!
Summary of Contents for CyFlow Space
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