CyFlow
™
Space | Operating Manual | March 2021
37
11.4
The Flow Cuvette
NOTICE
The flow cuvette is “the heart” of your flow cytometer. It is responsible to
guide cells and particles through the centre of the illumination spots with
a micrometre precision. The flow cell is manufactured with highest
possible precision and should be treated like that. Any deformation will
degrade the flow precision and make it irreparably unusable.
Figure 15:
Flow cuvette with sample, sheath fluid and waste connections.
WARNING
This device is a class 1 laser product, while the housing is unopened. If
the housing is opened and laser light is emitted, the device is a class 3b
laser product. Laser light exposure causes damage to skin and eyes.
Avoid
direct
exposure.
Do not open the device without authorization by Sysmex Partec.
NOTICE
Air bubbles usually enter the flow cuvette through the sheath fluid inlet.
Bubbles might stick inside the conical section above the sample nozzle
and do not enter the flow channel of the quartz cuvette segment. The
laminar flow of sheath fluid and sample flow as well as hydrodynamic
focusing are disturbed and broad peaks appear in the histograms.
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