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magnification and convert the image into a field of view easily seen by the 
human eye. 
 

COVER GLASS 

– thin glass cut in circles, rectangles, or squares, for 

covering the specimen (usually a thickness of 0.15 to 0.17mm). The 
majority of specimens should be protected by a cover glass, and must be 
covered when using 40XRD or 100XRD objectives. 
 

DEPTH OF FOCUS 

– the ability of a lens to furnish a distinct image 

above and below the focal plane. Depth of focus decreases with the 
increase of numerical aperture or with the increase of magnification. 
 

DIN 

– (Deutsche Industrial Normen 

originally

 

Deutsches Institut für 

Normung)

A German standard for the manufacturing of microscope 

lenses.  DIN lenses will be interchangeable from one DIN microscope to 
another.  
 

EYE POINT or EYE RELIEF

 

– the distance from the eye lens of the 

eyepiece to your eye where a full field of view is seen. 
 

FIELD OF VIEW 

– the area of the object that is seen when the image is 

observed. It may range in diameter from several millimeters to less than 
0.1mm. 
 

FOCAL LENGTH

 

– parallel rays of light after refraction through a lens will 

be brought to a focus at the focal point.  The distance from the optical 
center of the lens to the focal point is the focal length. 
 

NUMERICAL APERTURE (NA) 

– a measure of an objective’s light 

gathering capabilities. The concept may be compared to the F-valve in 
photographic lenses. Generally speaking, N.A. values of less than 1.00 
are "Dry" objectives. Values of 1.00 or greater require oil as a medium. 
Please note that condensers are part of the optical system and are also 
assigned an N.A. value. That value must be at least as high as that of the 
highest objective used. 
 

PARFOCAL

 

– a term applied to objectives and eyepieces when 

practically no change in focus is needed when changing objectives.

  The 

objectives on your microscope are parfocalized at the factory so that 

only a slight adjustment of the fine focus knob is needed to maintain focus 
when switching magnification. 
 

RESOLUTION or RESOLVING POWER

 

– the ability of a lens to define 

the details of the specimen at a maximum magnification.  This is governed 
by the NA (Numerical Aperture) of the lens.  For example, a 40X objective 

Summary of Contents for M10D Series

Page 1: ...Swift M10D Series Digital Microscope Use and Care Manual SWIFT OPTICAL Enduring Quality and Technical Excellence ...

Page 2: ...ewing and durable construction to withstand the rigors of a busy clinical practice or lab A built in 3MP digital camera enables users to display capture pictures or capture video clips of live images Illuminator Objective Slide Holder Head Eyepiece On Off Switch Illuminator Rheostat Iris Diaphragm Mechanical Stage Control Coarse Focus Control Diopter Adjustment Fine Focus Control Arm Base Nosepiec...

Page 3: ...S MICROSCOPE 1 Remove head and body from styrofoam packaging 2 Remove both shipping blocks from the body of the microscope as pictured below 3 Secure the head to the body of the microscope with the head locking screw as pictured below ...

Page 4: ...ension control to prevent stage drift CONDENSER the function of the condenser is to provide full illumination to the specimen plane and to enhance the resolution and contrast of the object being viewed The standard condenser of the M10D Series has a numerical aperture of 1 25 with filter carrier and iris diaphragm It is mounted in a sub stage focusing assembly that can be raised or lowered for pre...

Page 5: ...aining reagents Before the advent of phase contrast such specimens could only be examined in transmitted light by closing down the substage condenser diaphragm to a small aperture The narrow cone of illumination produced diffraction with destruction of detail SIEDENTOPF a binocular head design where the interpupillary adjustment increasing or decreasing the distance between the eyepieces is achiev...

Page 6: ...l millimeters to less than 0 1mm FOCAL LENGTH parallel rays of light after refraction through a lens will be brought to a focus at the focal point The distance from the optical center of the lens to the focal point is the focal length NUMERICAL APERTURE NA a measure of an objective s light gathering capabilities The concept may be compared to the F valve in photographic lenses Generally speaking N...

Page 7: ...precise movement and scanning of the slide 3 Rotate the nosepiece to place the lowest power objective 4XD over the specimen Be sure the objective clicks into position 4 Adjust the interpupillary distance of the Siedentopf binocular head for a comfortable view Align the eyepiece tubes of the binocular head to create one perfect circle by moving the eyepiece tubes in an arc motion 5 While viewing th...

Page 8: ...on objectives The objectives are parfocalized so that once the lowest objective 4XD is focused only a slight turn of the fine focusing knob is required when changing to 10XD 40XRD and 100XRD objectives Oil Immersion It is desirable to use immersion oil with the 100XRD objective Oil generates a fine resolution and brightness of the image viewed through the microscope Drop a tiny amount of oil 1 dro...

Page 9: ...r the visualization of stained cells To start you will need to make sure the condenser is in the highest position Use of the iris diaphragm is highly recommended to aid in adding contrast Rotate the condenser disk to the BF designation 4x 10x 40x Objectives Adjust focus and iris as normal 100x Objective Adjust and focus as normal It is highly recommended that immersion oil be used on the surface o...

Page 10: ...s to be softened with a neutral diffusing filter when using the 10X objective Place the neutral diffusing filter in the secondary filter carrier Rotate the disc to the desired phase magnification 10 20 40 or 100 to match the objective you are using Please note the 4x objective included with the system is not phase 10x and 40x Objectives Adjust and focus as normal Iris must be fully opened 100x Obj...

Page 11: ...us baseline Begin by rotating the condenser control knob to move the phase condenser to its highest point Rotate the phase turret control disc to the Bright Field BF setting Ensure that the disc clicks into position Rotate the 10x phase objective into the optical path Place a standard prepared specimen slide cover slip facing upwards on the stage Use the microscope focus controls to bring the spec...

Page 12: ... one hand grasp the very top portion of the centering telescope with the other hand look through the eyepiece of the centering telescope while slowly sliding the telescope tube out until the phase ring in the objective is in focus Tighten the knurled locking screw 10X Phase Begin by placing the condenser disk of the phase condenser on the 10 position Ensure that it is clicked into position the 10X...

Page 13: ...rings are superimposed If not perform adjustments as described above Please note As the magnification of the objective increases the number of concentric rings will also increase To determine which ring you are moving turn the adjusters to elicit movement Once you have determined which ring represents your condenser annulus align this to the rings in your field of view to achieve the proper alignm...

Page 14: ... the Motic Images Plus program and click on the CAPTURE WINDOW icon 6th icon from the top left or click on File then Capture Window to view a live image 2 The background balance setting will need to be adjusted to compensate for any uneven illumination light patterns Place a slide on the stage Move the specimen out of the field of view so an empty blank spot of the slide is being displayed Click o...

Page 15: ... file your images Full Help Menu The full software manual for Motic Images is accessible within the software s main page To begin open the Motic Images Software At the top of main screen find the menu tab labeled Help Click on Help and then select the help option This will open the Motic Images help file contents containing the full help menu ...

Page 16: ...pture your images Full Help Menu The full Live Imaging Module manual is accessible within the live Imaging main page To begin open the Motic Images Software At the top of main screen find the menu tab labeled File and click on Capture Once the Motic Live Imaging Module has opened click on Help This will open the Motic Live Imaging Module help file containing the full help menu ...

Page 17: ...ware please refer to the Motic Help files These files will help explain the functions of software There are help files for both the main Motic Images software window as well as the Motic Images Live Imaging window MOTIC IMAGES 3 0 HELP GUIDE To access the Motic Images 3 0 help menu click on Help located at the top of the Motic Images software screen Once the Help window open you will find the help...

Page 18: ...Motic Live Imaging Module Help To access the Motic Images Live Imaging help menu click on Help located at the top left hand side of the screen Once the Help window open you will find the help guide within ...

Page 19: ...ty See Care and Cleaning Section B PROBLEM Hairs or dust seem to be moving in the image CORRECTION The iris diaphragm is not open wide enough Slowly open the iris diaphragm to increase the size of the opening allowing for additional illumination C PROBLEM Unable to bring specimen into focus with any objective CORRECTION Eye lens of the eyepiece is partially unscrewed Remove the eyepiece and screw ...

Page 20: ...ning method depends on the nature of the optical surface and type of dirt Dirtiness on the image may be caused by the following variables Dirt on the outer or inner eyepiece lens Dirt on the front lens of the objective Dirt on the upper lens of the condenser Dirt on the surface of the sample slide glass Dirt on the upper lens of illuminator Dirt on other optical components of the microscope such a...

Page 21: ... with immersion oil it is essential to clean them after each observation session To clean use a cleaning cloth for lenses slightly dampened with a low graduation of alcohol Proceed by cleaning the frontal objective lens normally 100X Oil It is important for those objectives that work at a very close distance to the sample For optical components such as eyepieces condensers filters etc we recommend...

Page 22: ...omponents of the microscope such as the ON OFF switch the dimmer the lamp holder If there are grease stains use the same cloth moistened with a low graduation of alcohol If you face any problems related to the maintenance of your microscope please contact us Our technicians will gladly help you solve your maintenance issue s CLEANING The front lens of the objectives particularly the 40XRD and 100X...

Page 23: ...ng storage in the cabinet LED REPLACEMENT The Swift M10D series is equipped with a 3 watt LED illumination system The life of the LED may vary depending on use and intensity To prolong the life of the LED you should always turn off the unit when not in use It is important that you only use a Swift replacement LED because it is integrated on to a circuit board This LED has been tested and approved ...

Page 24: ...IFT OPTICAL INSTRUMENTS INC LIMITED LIFETIME WARRANTY Please see our website www swiftoptical com for complete warranty details and exclusions Swift Optical Instruments Inc 877 967 9438 www swiftoptical com ...

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