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MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 4.04 (20210122) 

 

 Never touch any part of the micromanipulator electromechanical device while it is in 

operation and moving. Doing so can result in physical injury (e.g., fingers can be caught 
and pinched between the moving parts of the micromanipulator). 

 

 As with all microinjection devices, sharp micropipettes can fly out of their holder 

unexpectedly. Always take precautions to prevent this from happening. Never loosen the 

micropipette holder chuck when the tubing is pressurized, and never point micropipette 
holders at yourself or others. Always wear safety glasses when using sharp glass 
micropipettes with pressure microinjectors. 

 

 Do not handle the manipulator mechanical while the power is on, and take care to 

ensure no cables pass close to the mechanical manipulator. 

Other 

 

Use this instrument only for microinjection purposes in conjunction with the procedures 
and guidelines in this manual. 

 

Retain the original packaging for future transport of the instrument. 

 

Some applications, such as piezo-impact microinjection call for the use of mercury in the 

micropipette tip. The use of any hazardous materials with any Sutter Instrument’s 
instrument is not recommended and if undertaken is done so at the users’ own risk. 

 

When transporting the mechanical manipulator, be sure to install the shipping screws 
supplied in their correct locations. Failure to do this may result in damage to the motors. 

 

This instrument contains no user-serviceable components — do not open the instrument 

casing. This instrument should be serviced and repaired only by Sutter Instrument or an 
authorized Sutter Instrument servicing agent. 

 

Sutter Instrument reserves the right to change specifications without prior notice. 

 

This device is intended only for research purposes. 

Handling Micropipettes 

 Failure to comply with any of the following precautions may result in injury to the users 

of this device as well as those working in the general area near the device.  

 

The micropipettes used with this instrument are very sharp and relatively fragile. 

Contact with the pulled micropipette tips, therefore, should be avoided to prevent 
accidentally impaling yourself.  

 

Always dispose of micropipettes by placing them into a well-marked, spill-proof “sharps” 
container. 

Summary of Contents for MP-285

Page 1: ...tal Drive Novato CA 94949 Voice 415 883 0128 Web www sutter com Fax 415 883 0572 Email info sutter com MP 285 Micromanipulator System Including Model MP 285A Controller ROE Basic Operations Rev 4 04 2...

Page 2: ...MP 285 SYSTEM ROE BASIC OPERATIONS REV 4 04 20210122 ii Copyright 2021 Sutter Instrument Company All Rights Reserved...

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Page 5: ...esigned SAFETY WARNINGS AND PRECAUTIONS Electrical Operate the MP 285 using 110 240 V AC 50 60 Hz line voltage This instrument is designed for use in a laboratory environment that has low electrical n...

Page 6: ...This unit is not designed for operation at altitudes above 2000 meters nor was it tested for safety above 2000 meters DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE CONTROLLER AND THE MECHANICAL...

Page 7: ...zo impact microinjection call for the use of mercury in the micropipette tip The use of any hazardous materials with any Sutter Instrument s instrument is not recommended and if undertaken is done so...

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Page 9: ...otating Dovetail Base 8 2 6 Minimizing Electrical Noise 9 3 MICROSCOPIC MANIPULATION 11 3 1 Some Basic Information 11 3 2 SETUP Selection 11 3 3 Changing Minimal Resolution 12 4 PULSE MOVEMENT 15 5 HO...

Page 10: ...r connections 4 Figure 2 5 Manipulator base dimensions 6 Figure 2 6 Headstage mounting plate 7 Figure 2 7 Mounting the headstage on the manipulator s Z axis 8 Figure 2 8 Adjusting the headstage angle...

Page 11: ...h emphasis on using the ROE Rotary Optical Encoder for user control These steps are in many ways identical to the steps performed any time one sets up an MP 285 for use at a microscope Many users will...

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Page 13: ...dovetail headstage mounting plate with plastic isolation plate Metal dovetail rod clamp 2 5mm hex wrench for removing the shipping screws 7 64 hex wrench for adjusting the rotating dovetail base MP 28...

Page 14: ...cessary strain 1 Connect the power cord to the MP 285 controller Make certain that the voltage selector next to the power cord socket on the back of the MP 285 controller has been set to the appropria...

Page 15: ...three axes Before the unit was shipped from Sutter its absolute origin location 0 0 0 was set at the center of travel of each axis This same location was also defined as Home This means that you can...

Page 16: ...the bearing ways A supply of four precision thickness washers is included with your MP 285 manipulator To limit misalignment you should use one on each of the four mounting screws between the MP 285 m...

Page 17: ...y to the rotating dovetail on the MP 285 Z axis Other headstages require use of the dovetail mounting plate dovetail mounting plate 2 middle D holes EPC 1 1 16 x 2 7 32 2 holes A B CV 4 1 1 8 x 1 15 1...

Page 18: ...igure 2 8 Adjusting the headstage angle To adjust the rotary dovetail to your desired angle 1 Loosen the brake set screw to rotate the headstage or pipette holder Failure to loosen the screw may lead...

Page 19: ...ulator will largely eliminate this noise source Ground tabs are located on each motor housing for this purpose It should be noted that the manipulator produces negligible electrical noise when it is n...

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Page 21: ...microsteps the minimal increment of manipulator movement per rotation of the ROE knobs Using simple commands you choose one of four different Setups that vary the number of microsteps per turn of the...

Page 22: ...the pipette If you wish to have even better control press the COARSE FINE button on the ROE see below Four factory installed Setups are selected from the main menu by pressing the corresponding numbe...

Page 23: ...3 2 Microns per ROE turn settings Setup Number Microns per ROE turn at 0 2 m 0 04 m 1 716 8 143 36 2 1024 204 8 3 512 102 4 4 102 4 20 48 Thus you have a relatively wide range of precision of movemen...

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Page 25: ...e unit is in continuous mode Pulse movement can be most easily observed under a microscope If you have already configured your microscope for micromanipulation as detailed in section Microscopic Manip...

Page 26: ...1 The speed of pulse movement is 5000 microsteps second for all Setups As there are two different resolutions this translates to the same two different speeds for each setup 1 m sec at coarse resoluti...

Page 27: ...the cursor indicated by the arrow to New Home Press ENTR the New Home menu will appear 5 Press ENTR to accept the default speed and resolution You will be back in the movement screen 5 2 Moving to the...

Page 28: ...xtend the same amount If you use a rotating device to position the manipulator try to install it such that rotation is up against a hard stop when the manipulator is in the operating location Note tha...

Page 29: ...advantageous to define this location as the absolute zero 0 0 0 for the MP 285 controller Once this is done it is straightforward to know where you are when you are looking at the coordinate display...

Page 30: ...ent press MOVE to return to the movement screen 2 Press to enter the Robotics menu TAB 3X to Learn and press ENTR to gain access to Learn functions You will be issued the following warning 3 If you ha...

Page 31: ...ogram To store your newly entered routine in a location other than program location 1 follow the steps detailed in the section Clear Program Location 1 for recording a new Robotic Series 6 5 Executing...

Page 32: ...ogrammed a multiple step withdrawal pattern to move your pipette out through a circuitous route you may want to use a similar pattern to move your pipette into the working area This can be accomplishe...

Page 33: ...gonal movements while in the movement screen 1 Press the remote NORM DIAG button on the encoder down and lit diagonal mode 2 The movement screen should now indicate that the controller is in diagonal...

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Page 35: ...ipulator Furthermore one can control the direction of movement commanded by a given input Axes assignment is covered in the manual under Controller Configuration and is adjusted using the menu command...

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Page 37: ...l void this warranty Warranty work will be performed only at the factory The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect after...

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Page 39: ...precautions v H home function 17 M mains fuses iii microscopic manipulation 11 movement amount per ROE turn 25 control of 25 N notes user 29 30 P precautions iii electrical iii pulse movement 15 R RO...

Page 40: ...MP 285 SYSTEM ROE BASIC OPERATIONS REV 4 04 20210122 30 NOTES...

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