Sutter Instrument MP-285, Basic Operations

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MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 4.04 (20210122)
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If these operations are part of your initial installation, your manipulator may not be mounted
in a position to allow you to center the pipette tip in the microscope field of view. If this is
true, you will have to stop and adjust the mounting location of the manipulator and/or the
angle of approach of the pipette and/or the mounting of the headstage in the rotary dovetail.
After repositioning, you will be able to bring the pipette to the correct working location. See
the
MP-285 Reference Manual
for additional information on pipette positioning and Sutter
accessories to aid in pipette access to different microscopes.
NOTE: Once you can see the pipette in your field of view, you are ready for
micromanipulation.
1.
Press <ESC> to get to the main menu
2. Press <4> to select Setup 4; the upper left-hand corner of the display should now read
S
⇒ 4.
3. Press <MOVE> to return to the movement screen.
4. Rotate the knobs on the ROE and observe the precise control you have over the
movement of the pipette. If you wish to have even better control, press the
<COARSE/FINE> button on the ROE (see below)
Four factory-installed Setups are selected from the main menu by pressing the corresponding
number (1 through 4) from the MP-285 keypad. Assuming you are in the movement screen
and you want to change the current Setup, the key sequence is as follows:
1.
From the movement screen press <ESC> to access the main menu
2. Press the number key <1> through <4> corresponding to the Setup you wish to use.
The actual values for number of microsteps per ROE turn are as follows:
Table 3-1. Microsteps per ROE turn settings
Setup
Number
Microsteps per
ROE turn
1 3694
2 5120
3 2560
4 512
3. Press <MOVE> to return to the movement screen and begin moving at the precision
specified by the selected Setup.
The selected setup is maintained when the MP-285 controller is turned off; you only need to
select a different setup when you wish to change precision of movement.
3.3 Changing Minimal Resolution
In addition to controlling the number of microsteps per ROE turn, you can also set the
microstep size to one of two values (coarse=0.2
µ
m or fine=0.04
µ
m). You then have two
different amounts of movement (ultimately two different precisions for each selected Setup).