ColorView III • Operation of the Shading Correction
10
Shading Correction
For a shading correction, the image errors are first determined in order to be
able to make corrections to the acquired image. Using the online shading
correction of ColorView III, these corrections already take place in the live-
image.
Operation of the Shading Correction
The correction of the image proceeds pixel to pixel with the aid of two differ-
ent reference image series: one consists of dark images for each resolution
of the camera, and the other consists of correction images. Before the online
shading correction is able to be used, you must acquire these reference im-
ages.
The illustration explains the
principle of the online shad-
ing correction.
Dark image and correction
image are transferred from
the PC to the camera based
on the magnification and ob-
servation method.
The corrections based on
these images are done in
the camera.
The image transferred to the
PC has already undergone
shading correction.
Dark Images
A dark image is an acquisition in which no light falls on the camera. Errors
can occur from noise or defective pixels which can be corrected by the dark
image. An own dark image is required for each resolution of the camera.
Correction Images
The illumination of the entire optical system without the object (with refer-
ence samples in reflected light mode) is shown. In addition to the camera
characteristics, the microscope's optical characteristics, especially the ob-
jective being used contribute to the correction image. A correction image for
each camera resolution and objective combination must be made accord-
ingly.
Acquisition Methods
The utilized acquisition method also plays a vital role in addition to the al-
ready mentioned factors of the actual cameras and objectives. The online
shading correction has been aligned with transmitted light, reflected light,
DIC and Phase Contrast microscopy. When acquiring the correction imag-
