Refrigerated Centrifuge Sigma 6-16KS
6 Using the centrifuge
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Version 06/2012, Rev. 1.11 of 06/04/2016 • sb
Translation of the original operating manual, part no. 0702202
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6.2.2.5
Tubes
• Load the tubes outside of the centrifuge. Liquids in the buckets or
multiple carriers cause corrosion .
• Fill the tubes carefully and arrange them according to their weight.
Imbalances result in the excessive wear of the bearings.
• In high-speed angle rotors, the vessels must be filled up to their useful
volume (= the volume stated for the vessel). If the vessels are only
partially filled, they will deform. This may result in leaks at the seals that
may become loose.
• When using glass tubes, the maximum value of 4,000 x g must not be
exceeded (except special high-strength glass tubes; please refer to the
information provided by the manufacturer).
• Follow the safety instructions and hazard warnings (see chapter 3 -
"Safety")!
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6.2.2.6
Blood bag systems
The centrifuge tolerates an imbalance of approx. 20 g. Therefore, it is
important to balance the buckets carefully.
• All four places of a rotor must be loaded with buckets. It is permissible
to fill just two opposite buckets with blood bag systems.
• The opposite buckets including the filled blood bag systems must have
an equal weight.
• The blood bags have to be put into the opposite buckets mirror-inverted.
• If in one bucket the main blood bag is on the left side, then the main
blood bag inserted in the opposite bucket has to be on the right side.
1
Filter
2
Blood bag
Fig. 11: Loading of blood bag systems
• When using smaller bag systems or in case of incompletely filled blood
bags adapters (e.g. part no. 17750) have to be inserted together with
the blood bag systems. This will avoid slipping of the bags which could
result in an imbalance.
• For the centrifugation of human blood suitable acceleration and
deceleration curves must be used. Curve no. 12 is the most suitable for
this purpose.
A german blood bank works successfully using the following data:
1
