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Sigma 146774, Operating Manual

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Sigma 146774 Operating Manual Download Page 3

 

Refrigerated Centrifuge with Heating System Sigma 3-30KHS 

  

 

 

 

 

Version 09/2014, Rev. 1.15 of 08/05/2020 

• sb 

3 / 91 

Translation of the original operating manual, part no. 0701402 

 

 

© Copyright by 
Sigma Laborzentrifugen GmbH 
An der Unteren Söse 50 
37520 Osterode am Harz 
Germany 
 
Tel.: 

+49 (0) 5522 / 5007-0 

Fax: 

+49 (0) 5522 / 5007-12 

Web: www.sigma-zentrifugen.de 
E-mail: [email protected] 
 

 

 

 

Summary of Contents for 146774

Page 1: ...e original operating manual sb 09 2014 Rev 1 15 of 08 05 2020 Sigma 3 30KHS part no 0701402 Sigma 3 30KHS from serial no 146774 Refrigerated Centrifuge with Heating System Operating Manual Please reta...

Page 2: ...Refrigerated Centrifuge with Heating System Sigma 3 30KHS 2 91 Version 09 2014 Rev 1 15 of 08 05 2020 sb Translation of the original operating manual part no 0701402...

Page 3: ...of 08 05 2020 sb 3 91 Translation of the original operating manual part no 0701402 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 F...

Page 4: ...Refrigerated Centrifuge with Heating System Sigma 3 30KHS 4 91 Version 09 2014 Rev 1 15 of 08 05 2020 sb Translation of the original operating manual part no 0701402...

Page 5: ...l force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety in...

Page 6: ...tallation of a standard angle rotor with a hermetic lid 30 6 2 2 3 Installing a rotor with the g lock system 31 6 2 2 4 Installation of accessories 31 6 2 2 5 Adapters 33 6 2 2 6 Vessels 33 6 3 Contro...

Page 7: ...8 1 3 Rotors buckets and carriers 63 8 1 4 Load bearing bolts 64 8 1 5 Glass breakage 65 8 2 Sterilisation and disinfection of the rotor chamber and accessories 65 8 2 1 Autoclaving 66 8 3 Service 67...

Page 8: ...Refrigerated Centrifuge with Heating System Sigma 3 30KHS Table of contents 8 91 Version 09 2014 Rev 1 15 of 08 05 2020 sb Translation of the original operating manual part no 0701402...

Page 9: ...a Standardm odule 010 Allgem eine Inform ationen 010 0020 Bestimm ungsgem e Verw endung Sigma 26 m od_1405318951312_68 docx 191709 2 1 1 2 Intended use Centrifuges are power driven machines that separ...

Page 10: ...odule 010 Allgemei ne Informati onen 010 0050 N ormen und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declaratio...

Page 11: ...e 020 Aufbau und Wirkungsw eise 020 0010 Aufbau der Zentrifuge 26 m od_1405318982945_68 docx 191793 2 1 2 1 Layout of the centrifuge Pos 17 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 3 30KS_3 30KH S...

Page 12: ...ypenschild 3 30KHS 13 mod_1342766672292_68 docx 68839 3 1 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal voltage 9...

Page 13: ...cally as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor...

Page 14: ...rifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram prov...

Page 15: ...1378 2 1 3 1 Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma Dangerous voltage I On Power Hot surface 0 Off Power Caution Risk of bruising Name pl...

Page 16: ...s operating manual the following names and symbols to indicate hazards are used DANGER This symbol stands for a direct hazard to the life and health of persons Non observance of these symbols causes s...

Page 17: ...y The centrifuge has to be maintained regularly see chapter 8 Maintenance and service Components that are not in a perfect state must be replaced immediately Pos 38 010 U niversalmodule Leerzeile 0 m...

Page 18: ...g in compliance with the relevant standards Pos 45 010 U niversalmodule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 46 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0...

Page 19: ..._0 docx 114 1 Pos 50 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0040 C hemische und bi ologische Sicher hei t 26 m od_1405319028141_68 docx 192101 3 1 3 6 4 Chemical a...

Page 20: ...ing vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of a standard rotor Observe the instructions on the instal...

Page 21: ...ion cracks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service lif...

Page 22: ...a dialog box see chapter 7 2 Table of error codes Pos 65 010 U niversalmodule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 66 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030...

Page 23: ...gen Sigma Standar dmodule 030 Sicher heit 030 0100 R estrisiken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The centrifuge was built in accordance with the state of the art and in co...

Page 24: ...m od_1405319161442_68 docx 192367 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage tem...

Page 25: ...KS_KHS berschrift 29 mod_1405321884012_68 docx 197967 2 1 4 5 Transport safety device Pos 89 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 3 30KS_3 30KH S 040 Lagerung und Tr ansport 040 0051 Transport...

Page 26: ...fficient time for drying min 24 h before the centrifuge can be used again Pos 94 010 U niversalmodule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 95 100 Sigma 100 BA Zentrifugen Sigma Standar dmod...

Page 27: ...pically the centrifuge must be protected with 16 Amp L or B fuses that are to be provided by the customer NOTE To ensure safe disconnection in the event of a fault an AC DC sensitive RCD residual curr...

Page 28: ...ndardm odul e 060 Betrieb_Spi ncontr ol L S 060 0020 Ei nschalten au er 6 16HS 27 mod_1405319201340_68 docx 192451 2 1 6 2 Switching the centrifuge on Press the mains power switch The display then ill...

Page 29: ...counter clockwise Lower the rotor with its central bore straight down onto the motor shaft Tighten the rotor tie down screw clockwise with the supplied rotor wrench with 7 5 Nm In doing so hold the ro...

Page 30: ...t the rotor tie down screw into the motor shaft Tighten the rotor tie down screw clockwise with the supplied rotor wrench with 7 5 Nm In doing so hold the rotor at its outer rim Follow the safety inst...

Page 31: ...ul e 060 Betrieb_Spi ncontr ol L S 060 0020 0020 0031 Einsetzen von Zubeh r 4pl tzig 3 30KS_KH S 27 mod_1405319206727_68 docx 192521 4 1 6 2 2 4 Installation of accessories Only use vessels that are s...

Page 32: ...loaded evenly It is not permissible to load angle rotors on only one axis correct incorrect Fig 8 Permissible and impermissible loading of an angle rotor and a swing out rotor example illustrations N...

Page 33: ...gen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S 060 0020 0020 0050 Gef e 2 16KL bis 4 5L 27 m od_1405319211622_68 docx 192577 4 1 6 2 2 6 Vessels Load the vessels outside of the centrifuge Li...

Page 34: ...mod_1405320193718_68 docx 193123 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several d...

Page 35: ...ifuge lid must be opened before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is t...

Page 36: ...he actual speed is displayed below this value The values are stated in revolutions per minute min 1 rpm and they depend on the RCF values see chapter 2 2 2 1 Speed radius and relative centrifugal forc...

Page 37: ...peed is reached Select the field Runtime and press the function knob The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 cloc...

Page 38: ...unction knob in order to accept the data Fig 13 Rotor selection list here for a swing out rotor Automatic rotor identification The centrifuge automatically identifies the rotor that is currently being...

Page 39: ...Standard menu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode...

Page 40: ...No message will be issued when the set temperature is reached A parameter is changed except for the temperature or another input is made In this case the RAPID_TEMP program will be aborted No message...

Page 41: ...runtime of the running centrifugation run For this purpose a green progress bar and percentage value are displayed in the program field Fig 16 Progress indicator during a centrifugation run After the...

Page 42: ...me and temperature exception the RAPID_TEMP program will not be listed The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be display...

Page 43: ...still Depending on the substances that are to be centrifuged it may make sense to bring the centrifuge to a certain temperature prior to starting a centrifugation The temperature adaptation prevents t...

Page 44: ...ward facing arrow in the speed field Values between 1 2 and 10 0 g cm3 are possible Centrifugation monitoring The centrifugation monitoring function enables the continuous monitoring of the speed and...

Page 45: ...ed if the current temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message wil...

Page 46: ...he Save function will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be displayed in the status line and the lock...

Page 47: ...ge It enables the optimum adaptation of the centrifuge to its specific area of application Fig 21 Menu Setup Function Open lid after run The automatic lid opening function ensures that the lid opens w...

Page 48: ...erature can be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follo...

Page 49: ...e entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in order to enter the next cha...

Page 50: ...u The curve editor will be displayed Select a curve number between 20 and 29 in the input field Curve If the curve number is already used the stored curve will be displayed The input field Int is used...

Page 51: ...e since the necessary slope 0 03 min 1 rpm which means that it is beyond the defined range Example 2 Start speed 0 min 1 rpm final speed 15000 min 1 rpm runtime 10 sec not possible since the necessary...

Page 52: ...BA Zentrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S Spincontrol S Zent7 060 0030 0030 Programmbetri eb 27 m od_1405320235093_68 docx 193459 3 1 6 3 3 Program mode A program contains a...

Page 53: ...to confirm the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm it The program will be...

Page 54: ...list and confirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The program will be executed and...

Page 55: ...n in the Setup menu Fig 28 Program rotation function When a program is loaded while the program rotation function is active this program will be used as the start program for the rotation After the co...

Page 56: ...tandard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 250V max 6 A part no 17702 Pos 160 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 161 010 Universa...

Page 57: ...nnection Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illuminated Several possible causes Power off on If the error occurs again contact servic...

Page 58: ...m the opening on the left side of the control panel e g with a screwdriver Fig 30 Position of the opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hol...

Page 59: ...centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance err...

Page 60: ...ent of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma z...

Page 61: ...al stress e g by heat generators Pos 176 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 177 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 080 War tung und Instandhaltung 080...

Page 62: ...5320618247_68 docx 194845 3 1 8 1 2 Accessories CAUTION For the care of the accessories special safety measures must be considered as these are measures that will ensure operational safety at the same...

Page 63: ...ts and carriers Rotors buckets and carriers are produced with the highest precision in order to withstand the permanent high stress from high gravitational fields Chemical reactions as well as stress...

Page 64: ...t of the buckets and therefore the smooth operation of the centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bol...

Page 65: ...Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the gre...

Page 66: ...Accessories Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 3...

Page 67: ...o withstand this high level of stress high quality components were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especial...

Page 68: ...ment we are obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and sp...

Page 69: ...afe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new pack...

Page 70: ...ong household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return...

Page 71: ...50 37520 Osterode Germany Type 3 30KHS Connection requirements Electr connection Protection class IP code see name plate I 20 Power consumption kW Input fuse AT 1 35 12 0 at 220 240 V 60 Hz temperatu...

Page 72: ...y of air 80 from 5 C up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 211 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx...

Page 73: ...www sigma zentrifugen de Pos 218 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 219 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 220 100 Sigma 100 BA Zentri...

Page 74: ...l operating manual part no 0701402 Pos 222 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 110 Anhang 110 0020 Dr ehzahl Schwer efeld Diagr amm 28 m od_1405320705642_68 docx 195153 2 1 11 2 Speed...

Page 75: ...ope of the fixed acceleration curves defines the time that is required to accelerate the rotor by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maxim...

Page 76: ...Appendix 76 91 Version 09 2014 Rev 1 15 of 08 05 2020 sb Translation of the original operating manual part no 0701402 Fig 38 Diagram of linear curves Fig 39 Diagram of quadratic curves Pos 225 010 Uni...

Page 77: ...dition 1 16K Edition 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 1...

Page 78: ...1 4 4 1 1 Acetone C3H6O 100 1 1 4 1 1 4 4 1 4 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Alumini...

Page 79: ...iaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylanili...

Page 80: ...2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1...

Page 81: ...30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric...

Page 82: ...91 Version 09 2014 Rev 1 15 of 08 05 2020 sb Translation of the original operating manual part no 0701402 Pos 230 100 Sigma 110 BA Zentrifugen Sigma Proj ekte 3 30KS_3 30KHS 110 Anhang 110 0060 Konfor...

Page 83: ...erated Centrifuge with Heating System Sigma 3 30KHS 11 Appendix Version 09 2014 Rev 1 15 of 08 05 2020 sb 83 91 Translation of the original operating manual part no 0701402 11 6 EC declaration of conf...

Page 84: ...Refrigerated Centrifuge with Heating System Sigma 3 30KHS 11 Appendix 84 91 Version 09 2014 Rev 1 15 of 08 05 2020 sb Translation of the original operating manual part no 0701402...

Page 85: ...d Centrifuge with Heating System Sigma 3 30KHS 11 Appendix Version 09 2014 Rev 1 15 of 08 05 2020 sb 85 91 Translation of the original operating manual part no 0701402 11 7 Declaration of conformity C...

Page 86: ...ated Centrifuge with Heating System Sigma 3 30KHS 11 Appendix 86 91 Version 09 2014 Rev 1 15 of 08 05 2020 sb Translation of the original operating manual part no 0701402 Ende der Liste f r Textm arke...

Page 87: ...entrifugation monitoring 44 Centrifugation of infectious toxic radioactive or pathogenic substances 19 Centrifugation principle 13 Centrifugation with low capacity 32 Centrifugation with vessels of va...

Page 88: ...or correction 57 Error message 57 Executing a program 54 Explanation of the symbols and notes 16 Explosive substances 19 External signal 49 F Filling quantity refrigerant 71 Fire preventions 19 Form f...

Page 89: ...Standard 36 Mode of operation 13 Modification mode active 35 Motor error 59 Motor shaft 29 30 61 Multiple carrier 33 N Name plate 11 12 No indication on the display 57 No power in the mains supply 57...

Page 90: ...30 33 Safety instructions for centrifugation 20 Safety of rotors and accessories 21 Safety chemical and biological 19 Safety electrical 18 Safety mechanical 18 Safety conscious work 17 Saving a progr...

Page 91: ...r chamber 22 Temperature monitoring 45 Temperature monitoring system 22 Temperature range 71 Temperature unit 48 Temperature value not reached 57 Thermal stress 61 Time fine 48 Time range 71 Toxic sub...

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