Shodex R I-50 1 EX Operation Manual Download Page 32 | Manualshive

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Shodex R I-50 1 EX Operation Manual Download Page 32

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Problem

Possible cause

Solution

Large baseline drift

1. Insufficient solvent

replacement.

2. Room temperature

fluctuates greatly.

3. Bubbles are present in

flow cell.

4. Flow line is contaminated.

1. Replace the solvent thoroughly.

(Refer to the notes in 6-4.)

2. Use detector in places where changes

in room temperature are small.

3. Pass thoroughly degassed eluent

through to purge bubbles.

4. Wash flow line thoroughly.

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Summary of Contents for R I-50 1 EX

Page 1: ...al CAREFULLY CAREFULLY CAREFULLY CAREFULLY before using Shodex8 RI 501 Refractive Index Detector ＲｅｆｒａｃｔｉｖｅＩｎｄｅｘＤｅｔｅｃｔｏｒＯｐｅｒａｔｉｏｎＯｐｅｒａｔｉｏｎＯｐｅｒａｔｉｏｎＯｐｅｒａｔｉｏｎＭａｎｕａｌＭａｎｕａｌＭａｎｕａｌＭａｎｕａｌＲＩ５０１ＥＸ R ...

Page 2: ...O LTD CO LTD CO LTD Printed in Japan All rights reserved Contents of this publication may not be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from the copyright owner ...

Page 3: ...ts accuracy or completeness Shodex RI 501EX Refractive Index Detector should only used for Liquid chromatography analysis Do not use it any other purpose This manual does not expect Shodex RI 501EX Refractive Index Detector to be used for clinical or medical purposes Shodex RI 501EX Refractive Index Detector must not be used for such purposes The content of this manual is subject to change without...

Page 4: ... the detector in places where combustible gas or any source of fire or of spark exists or might exist Prior to connection make sure that the voltage of the power socket into which the detector power cable is plugged is the same as the power supply voltage indicated on the detector The type of the power socket into which the detector power cable is plugged should be of a 3P type with a grounding te...

Page 5: ...of dust exist Do not connect any tube other than the provided OUT tube to the eluent outlet joint of the detector Put the exit end of the OUT tube in the waste liquid bottle and do not apply back pressure When connecting this detector to other detectors in series put it at the end If eluent freezes inside the detector the flow line might break damaging the detector If there is any chance of eluent...

Page 6: ...ing organic solvent with an eluent containing salt wash the line with acetone first and then pure water before filling the line with the salt containing eluent When high voltage cause by static electricity applies to the instrument there are possibilities some incorrect actions are observed Please notice the static electricity Please do not set other equipments on this detector because of avoiding...

Page 7: ...d Connection 1 1 1 16 6 6 6 ６１ Power Connection and Grounding 16 ６２ Signal cable Connections 17 ６３ Tube Connections 18 ６４ Operation Procedures 19 ７ Care And Maintenance 21 21 21 21 ７１ Flow Line Cleaning 21 ７２ Replacing Fuse 21 ７３ Cleaning of detector exterior 21 ８ Validation 22 22 22 22 ９ Troubleshooting 2 2 2 24 4 4 4 10 WEEE Instructions 2 2 2 26 6 6 6 Appendix 1 Property Data of Solvents...

Page 8: ...e 2 core shield cable approx 2m 1 3 IN tube set Stainless steel tube of 1 6 mm outer dia x 0 25mm inner dia x 1000 mmLength setscrew and ferrule attached 1 4 OUT tube set Teflon tube of 2 5 mm outer dia x 1 5 mm inner dia x 2000 mmLength connecting tube setscrew and ferrule attached 1 5 Discharge tube set Silicone rubber tube of 10 mm outer dia x 7 mm inner dia x 2000 mmLength connection joint att...

Page 9: ... and excellent stable baseline performance 2 Safety Solvent leak sensor generates an output signal that stops pump flow in case of an eluent leak within the detector Relief valve provided in the flow path to prevent damage such as a solenoid valve by overpressure 3 Integration As well as an LAN communication port the detector is fitted with signal input and output terminals which permit highly aut...

Page 10: ...p FUSE Note External temperature control is set to a temperature 5 lower than the set temperature 15 Operator Support Automatic Start Up Start Up Sequence Span Validation Guide 16 External Communication LAN 17 Cell Volume 8 μ L 18 Maximum Flow Rate 10 mL min mobile phase pure water 19 Pressure Rating 50 kPa 0 5kgf cm2 20 Internal Volume Inlet Port Flow Cell approx 80 μ L Flow Cell Outlet Port appr...

Page 11: ...is refracted twice according to the difference of the refractive index For this reason the RI 501EX provides twice the sensitivity of conventional flow cell having a single interface 1 Light beam 2 Sample cell 3 Reference cell 4 Light beam at the 5 Light beam at the 6 Displacement 7 Photo sensor ns Refractive index of liquid in sample cell nr Refractive index of liquid in reference cell Fig 4 1 Pr...

Page 12: ...the refractive index Fig 4 3 Movement of the image on the photo sensor 4 4 4 4 2 2 2 2 Flow Flow Flow Flow L L L Line ine ine ine As shown in Fig 4 4 below the flow line is so designed as to enable replacement of a reference liquid by only pushing a button Fig 4 4 Flow Line Panel From column To waste liquid receptacle 4 3 4 OUT IN COM NO NC 1 5 6 7 NO Normally Open NC Normally Close 2 1 3 2 1 2 3 ...

Page 13: ...ing circuit with display and keyboard a temperature control circuit and a light intensity control circuit The temperature control circuit and light intensity control circuit are controlled by the signal processing circuit Fig 4 5 shows electrical system block diagram of the RI 501EX 1 Light source 9 Temperature sensor internal 17 Recorder Output 2 I V converters 10 Temperature fuse internal 18 LAN...

Page 14: ...On Off Event Marker and Auto Zero Optical Auto Zero is carried out by activation of the null Glass driver and fine adjustments are made through calculation Execution of commands given via external device e g purge on off auto zero Displaying data and status information such as Refractive Index Temperature Signal outputs displays various error messages such as Temperature control Light Intensity co...

Page 15: ...ge the flow path When the valve is on the LED above the key will be lit and the eluent will flow through the reference cell chambers Press to turn off the valve Marker key Press key to generate an event marker signal and add it to the recorder output During this function the LED above the key will be lit Leak LED Leak Illuminated when solvent leak is detected Auto Zero Key Press the key to activat...

Page 16: ...s or settings Inlet Port IN Connects tubing from separation column outlet Outlet Port OUT Eluent passing through the flow path is discharged from this port 5 5 5 5 2 2 2 2 Side Panel Side Panel Side Panel Side Panel Fig 5 2 Side panel of Shodex RI 501EX No Name of parts Function Drain Port In case of internal eluent leak the eluent will be discharged From this port Connect the attached tubing as n...

Page 17: ... RAM Parameter Home Position Overheating Optical Balance Intensity Leak out terminals LEAK OUT A contact signal is sent out through these terminals when an eluent leak is detected Ready out terminals READY OUT A contact signal is sent out through these terminals when 10 minutes have passed since the temperature of the optical system reached the designated value Recorder terminals REC OUT Signals t...

Page 18: ...arameter setting screen Validation screen 123 4uRIU 40 2 PARAMETERS VALIDATION TEMP UNSTABILIZE SETTING Monitor screen 26 27 28 29 ＸＸＸＸｕＲＩＵＸＸﾟＣＡＡＸＸＡＡＡＡＡＡＡＡＡＡＡＡＡＡＡＡ 30 No Function This indicates the refractive index in RIU unit This indicates the temperature of the optical system One of the following is displayed in response to the detector status 1 LK Key Lock status 2 ER Error status 3 RD A...

Page 19: ... or dirt 10 TEMP UNSTABLE After the detector is started this remains displayed until the temperature remains within 1 C of the set temperature for 10 minutes 11 Cannot set the LAN unit Push the Enter Key Is displayed when a LAN setting error has occurred Parameter setting screen To show the currently set parameters in order press the key or the key while the parameter setting screen is displayed V...

Page 20: ... S Seq key to start and stop the S SEQ Press The Enter key to display the result of the validation Each S SEQ mode is changed with the ENTER key and Arrow Keys The S SEQ mode is displayed by a screen2 Only in CUSTOM mode screen 25 is displayed from screen 19 ...

Page 21: ...RIU 1024mV 256 3 TEMPERATURE OFF 30 55 1 Step 40 4 TIME CONSTANT 0 1 0 25 0 5 1 1 5 2 3 6 8 Steps sec 1 5 5 POLARITY 6 BASELINE SHIFT 0 200 １ Step 50nRIU 0 7 S SEQ MODE FINE STANDARD COARSE CUSTOM STANDARD 8 LEAK SENSOR ON OFF ON 9 KEY LOCK YES NO NO 10 IP ADDRESS 0 0 0 0 255 255 255 255 192 168 1 2 11 SUBNETMASK 128 0 0 0 255 255 255 254 255 255 255 0 12 USE DEFAULT GATEWAY YES NO NO 13 DEFAULT G...

Page 22: ...lt setting is 30 seconds With this setting the valve remains on for 15 seconds and off for another 15 seconds Choose preferred setting Custom if necessary 2 Number of Cycle 3 3 9 This sets the number of the purge cycle 3 Time to Auto Zero 240 sec 60 990 sec At the end of the purge cycles the auto zeroing should be made This defines the length of the pause period between the end of the purge cycles...

Page 23: ...r cable is plugged should be of a 3P type with a grounding terminal Other type of power socket should not be used The accessory power cable should be used to connect the detector to the power socket Other cable should not be used Do not use the detector in places where combustible gas or any source of fire or of spark exists or might exist As the detector is readily affected by the ambient tempera...

Page 24: ...clear wire to FG Inserts the end of lead wire while pressing the button with driver Then remove the driver to lock the wire Make sure the wire is firmLy grabbed connect the wire disconnect the wire For the contact signal out Ready Solvent Leak and Error cable connection please use duplex lead wire External input output Auto Zero In Purge In Ready Out Leak Out and Error Out are for contact closure ...

Page 25: ...t joint to the outlet of the column to be used through the provided IN TUBE When fastening the stainless steel set screw of the IN TUBE grip the eluent inlet joint with a spanner 2 Connect the supplied OUT TUBE to the eluent outlet joint Do not connect any tube other than the provided OUT TUBE to the Eluent outlet joint of the detector Put the exit end of the OUT TUBE in a waste liquid receptacle ...

Page 26: ...rget values during Measuring Time the sequence is complete and the RD sign will come up on the Monitor screen Manual Start Manual Start Manual Start Manual Start 1 Set the parameter on operating parameter setting screen Refer to 5 4 2 Start pumping mobile phase solvent at flow rate 1 mL min to reference cell Purge On 3 Press key in every 10 seconds to on off the purge valve for few minutes 4 Keep ...

Page 27: ...a thoroughly degassed eluent It is recommended that a degassing device be used to degas the eluent as it is easy to use and permits continuous degassing If an eluent is replaced with another eluent in which it is insoluble after having withdrawn the old eluent from the line wash the line with a solvent which is soluble in both eluents then fill the line with the new eluent For example if water is ...

Page 28: ... being replaced power off the detector immediately and disconnect the power cord Please consult our local representative in your area or SHOKO SCIENCE CO LTD 7 7 7 7 3 3 3 3 Cleaning of detector Cleaning of detector Cleaning of detector Cleaning of detector exterior exterior exterior exterior When the exterior such as top cover of front panel were stained or got wet for whatever reasons please kee...

Page 29: ... will appear in the Monitor screen LAMP VOLTAGE This indicates the voltage applied to the lamp When the Voltage falls 4 9V a message GOOD will appear in the screen DRIFT This indicates a result of S SEQ When the drift falls set value a message GOOD will appear in the screen NOISE This indicates a result of S SEQ When the noise falls set value a message GOO D will appear in the screen SPAN This ind...

Page 30: ...en and press the key to memorize the original 0μ RIU baseline The screen will move to the sample measurement screen automatically after storing the original baseline information 6 Disconnect and remove the tube from the inlet port of the detector 7 Make sure that the purge valve is turned off 8 Fill a syringe with the standard sucrose solution and gently inject the solution through the inlet port ...

Page 31: ...ive to perform one passage of methanol Periodic noises are generated 1 Pulsation by pump is large 2 Existence of bubbles in the flow cell enlarges the pulsation by pump 1 Install device such as damper and resistance pipe to eliminate pulsation close to delivery side of pump 2 Pass thoroughly degassed eluent through at flow rate of about 1mL min While pushing the key repeatedly into ON and OFF posi...

Page 32: ...erature fluctuates greatly 3 Bubbles are present in flow cell 4 Flow line is contaminated 1 Replace the solvent thoroughly Refer to the notes in 6 4 2 Use detector in places where changes in room temperature are small 3 Pass thoroughly degassed eluent through to purge bubbles 4 Wash flow line thoroughly ...

Page 33: ...ched the end of its life instrument has reached the end of its life instrument has reached the end of its life instrument has reached the end of its life They will advise They will advise They will advise They will advise you regarding the instrument treatment you regarding the instrument treatment you regarding the instrument treatment you regarding the instrument treatment The objective of this ...

Page 34: ...62 2 1 4 07 1 2 8 080 0 0 9 Ethyl bromide 0 37 1 424 511 1 6 7 11 3 3 8 037 8 1 4 Ethyl ether 0 38 0 23 1 353 220 45 0 180 0 1 9 48 0 2 6 035 0 0 7 Ethyl sulfide 0 38 0 45 1 442 290 Chloroform 0 40 0 57 1 443 245 Methylene chloride 0 42 0 44 1 424 245 50 0 518 9 3 8 15 4 2 2 038 5 0 9 Methyl i butyl ketone 0 43 1 394 330 Tetrahydrofuran 0 45 1 408 220 14 4 321 1 2 0 11 8 2 5 066 1 0 9 Ethylene dic...

Page 35: ...y SD122810 Limit switch assembly SD122900 Photo sensor assembly SD122000 Heater set Internal SD122010 Heater set External SD122220 I V circuit board SD123000 Leak sensor board SD122310 Measurement board SD122420 Joint Panel Cover SD122430 Power Switch key SD122440 LCD unit SD122450 Key board SD122470 Membrane Key Panel Assembly SD122600 Switching Regulator SD100100 Power cable 125V SD100110 Power ...

Page 36: ...i Road Jing An Shanghai 200041 P R China Tel 86 0 21 6217 6111 Fax 86 0 21 6217 9879 E mail sales shodexchina com Tel 1 212 370 0033 Fax 1 212 370 4566 E mail sales shodexhplc com URL www shodexhplc com URL www shodex com cn KOREA KOREA KOREA KOREA Shoko Shoko Shoko Shoko Kor Kor Kor Korea ea ea ea Co Co Co Co Ltd Ltd Ltd Ltd E E E EU U U UR R R RO O O OP P P PE E E E A A A AF F F FR R R RI I I IC...

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