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If any eluent freezes inside the detector, the flow line might break
damaging the detector. If there is any chance of the eluent freezing
during stoppage or storage of the detector, withdraw the eluent
completely from the flow line of the detector.
When using any eluent containing a salt of high concentration,
make sure that the flow line is washed with water thoroughly after use.
Failure to do so may result in the plugging of the line causing the
detector to cease operation.
If the detector is not to be used for more than one week, then, prior to
storage, wash the flow line with pure water and acetone, and dry the line
by allowing nitrogen gas to flow through.
Do not use any eluent, including hydrochloric acid, which might corrode
the material, such as stainless steel, that it comes into contact with.
Use of such eluent might cause a base line drift and damage the detector.
Use a thoroughly degassed eluent.
It is recommended that a degassing device be used to degas
the eluent as it is easy to use and permits continuous degassing.
If an eluent is replaced with another eluent in which it is insoluble,
after having withdrawn the old eluent from the line,
wash the line with a solvent which is soluble in both eluents,
then fill the line with the new eluent.
For example, if water is replaced with chloroform, wash the line with
acetone before filling the line with chloroform.
When replacing an eluent containing salt with an eluent containing
organic solvent, wash the line with pure water and then acetone
before filling the line with the organic-solvent-containing eluent.
In contrast, when replacing an eluent containing organic solvent
with an eluent containing salt, wash the line with acetone first and
then pure water before filling the line with the salt-containing-eluent.
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