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S.Seq
It is recommended that the HPLC system be validated from time to time to maintain
the accuracy and reliability of analysis. Thin task of validation has been made easier
due to the unique and user-friendly feature of
Shodex
RI-501EX
.
SPAN CHECK
Press the key to obtain the “SPAN VALIDATION”
screen.
（Refer to the following for the details.）
S.SEQ
Press the
key once to call the start-up sequence.
The sequence is complete and the sign(
RD
or“FAIL”) will
come up on the Monitor screen.
DISPLAY
TEMPERATURE
This indicates the difference between the set temperature
and the actual temperature in degree Celsius.
When the difference becomes
within
“+or -”1 degree,
a message(“GOOD”) will appear in the Monitor
screen.
LAMP VOLTAGE
This indicates the voltage applied to the lamp. When the
Voltage falls 4.9V, a message(“GOOD”) will appear in
the screen.
DRIFT
This indicates a result of
“
S.SEQ
”
.
When the drift falls
set value
, a message(“GOOD”) will appear
in the screen.
NOISE
This indicates a result of
“
S.SEQ
”
.
When the noise falls
se t va lue
, a message(“GOO
D”) will appear in the screen.
SPAN
This indicates a result of
“
SPAN CHECK
”
.
Please check whether the display value is "243-
268" μRIU.
Do the Span Validation, follow the procedures below :
1) Preparation of standard sucrose solution :
Weigh 175 mg of sucrose and transfer it to a 100 mL volumetric flask.
Dissolve it with deionized, filtered, degassed water until the solution reaches the
mark on the flask.
Always use freshly made sucrose solution.
Enter
8
8
8
8.
.
.
. Validation
Validation
Validation
Validation
Note
Note
Note
Note