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P a g e
Begin Day Temperature
Calibration
Begin Night Temperature
Calibration
USER MAINTENANCE
Temperature Stabilization
The chamber air temperature must stabilize in order to perform an accurate calibration. Additionally,
the unit must be calibrated twice — once at your application day temperature and then at your
application night temperature to compensate for the extra heat generated during the day mode by
the day illumination bulbs in the chamber. Calibrate twice, even if the day and night temperatures of
your study are the same.
The calibrations must be carried out with the unit running in its manual mode. Accurate calibrations
cannot be carried out while the auto cycle is running. Calibrating in the manual day and night modes
will also calibrate for the auto cycle day and night phases.
1.
After introducing and placing the temperature probes, place the unit into manual day mode
(page 26, steps 1 - 8). The day temperature set point should be that of your application day
period.
o
This is the day temperature you will be running the unit at during your study,
regardless if it will be operating in the auto cycle phases or a manual mode.
2.
Allow the unit to operate undisturbed for
8 hours
in the manual mode at your application
day temperature—for example overnight—before starting the calibration when first putting
the unit into operation in a new environment.
o
To be considered stabilized the unit must operate at your day calibration
temperature with
no fluctuations of ±0.5°C or greater.
3.
See next page for a suggested calibration procedure.
Night Temperature Calibration
4.
Following a successful day calibration, place the unit in its manual night mode (page 52). The
temperature of the manual night mode should be set to night temperature of your
application.
5.
Allow the unit to operate undisturbed for
a minimum of 2 hours
to achieve and stabilize at
the night temperature.
o
To be considered stabilized the unit must operate at your night calibration
temperature for at least 1 hour with
no fluctuations of ±0.5°C or greater
.
Required day temperature stabilization
period with the incubator door closed
Incubator Powered
Fluctuations
(Exaggerated)
Day Calibration
Completed
Place unit in manual night mode
Door remains closed and sealed
