shelolab SRI21D Installation & Operation Manual Download Page 2

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P a g e

Not for Fly Cultivation

This unit is not designed to hold

fruit flies

(

Drosophila melanogaster)

. Use with flies voids the

manufacturing warranty and risks damaging the unit.

Other incubator models in the SRI family are manufactured specifically for fly applications. Talk to

your distributor or customer service representative to identify a model compatible with your study or

production model.

Summary of Contents for SRI21D

Page 1: ...DIURNAL PLANT GROWTH CHAMBER 115 Voltage Installation Operation Manual SRI21D ...

Page 2: ...aster Use with flies voids the manufacturing warranty and risks damaging the unit Other incubator models in the SRI family are manufactured specifically for fly applications Talk to your distributor or customer service representative to identify a model compatible with your study or production model ...

Page 3: ...nt with the following standards for use within an ambient air pressure range of 22 14 31 3 inHg 75 106 kPa with no flammable volatile or combustible materials being heated CAN CSA C22 2 No 61010 1 2012 CAN CSA C22 2 No 61010 2 010 2004 Reaffirmed 2014 07 UL 61010 1 2012 05 UL 61010A 2 010 2002 03 EN 61010 1 2010 EN 61010 2 010 2014 Supplemented by UL 61010 2 010 2015 ...

Page 4: ... Cleaning and Disinfection 18 GRAPHIC SYMBOLS 19 CONTROL PANEL OVERVIEW 21 OPERATION 23 Theory of Operation 23 Put the Chamber into Operation 25 Set the Manual Day Mode Temperature Set Point 26 Set the Manual Night Mode Temperature Set Point 29 Program Clock Times and Cycle Temperature Set Points 32 Load the Chamber 38 Condensation and the Dew Point 38 Launching the Day Night Cycle 39 Accessory Co...

Page 5: ...ervisors must draw up a site specific standard operating procedure SOP covering each application and associated safety guidelines This SOP must be written and available to all operators in a language they understand Intended Applications and Locations The incubators are intended for constant temperature non humidified general incubation applications in professional industrial and educational envir...

Page 6: ...800 322 4897 extension 4 or 503 640 3000 FAX 503 640 1366 Sheldon Manufacturing INC P O Box 627 Cornelius OR 97113 ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its products As a result engineering changes and improvements are made from time to time Therefore some changes modifications and improvements may not be covered in this manual If your unit s operating characte...

Page 7: ...on chamber through the unit access port Select a thermocouple suitable for the application temperature you will be calibrating at Why a Probe Reference readings taken outside the chamber using wire temperature probes avoid chamber door openings Openings disrupt the chamber temperature Each disruption requires a minimum 1 hour wait to allow the atmosphere to re stabilize before continuing No Alcoho...

Page 8: ...8 P a g e INTRODUCTION ...

Page 9: ...exterior If you find any damage to the unit follow the carrier s procedure for claiming damage or loss 1 Carefully inspect the shipping carton for damage 2 Report any damage to the carrier service that delivered the unit 3 If the carton is not damaged open the carton and remove the contents 4 The unit should come with an Installation and Operation Manual 5 Verify that the correct number of accesso...

Page 10: ...10 P a g e Figure 1 SRI21D RECEIVING YOUR UNIT ORIENTATION PHOTO Control Panel Temperature Probe Wire Shelf 1 of 4 Chamber Door Cultivation Chamber Door Gasket Day Period Fluorescent Illumination Blubs ...

Page 11: ... DATA PLATE INFORMATION The unit data plate contains the chamber model number and serial number Record this information below for future reference The data plate is located on the unit exterior on the top right side Model Number Serial Number ...

Page 12: ...12 P a g e RECEIVING YOUR UNIT ...

Page 13: ...environment page 14 Check that a suitable electrical outlet and power supply is present page 15 Install the Chamber in a suitable workspace location Review the lifting and handling instructions page 15 Install the chamber in its workspace location page 15 Make sure the chamber is level page 16 Set up the Chamber for use Install the chamber door handle page 16 Verify that all packaging has been rem...

Page 14: ...e conditions may adversely affect its temperature range and stability ENVIRONMENTAL DISRUPTION SOURCES When selecting a location to install the chamber consider all environmental conditions that can affect the unit temperature performance For example Proximity to ovens autoclaves and any device that produces significant radiant heat Heating and cooling ducts or other sources of fast moving air cur...

Page 15: ... The unit must be positioned so that all end users can quickly unplug the power cord in the event of an emergency The unit is provided with an integral 125V 15 Amp NEMA 5 15P 8ft 2 5m power cord Circuit Breaker The unit is provided with an integral circuit breaker to protect against overcurrent conditions Always determine the cause of an overcurrent event before resetting a tripped circuit breaker...

Page 16: ...e previous entries of the Installation section LEVELING The unit must be level and stable for safe operation Ensure that the chamber is placed on a flat and level surface prior to placing the unit in operation ATTACH THE CHAMBER DOOR HANDLE Attach provided the door handle to the chamber door Use the instructions packaged with the handle ...

Page 17: ...ge is used to secure the shelves during transit and prevent damage to the chamber interior 1 Remove all shipping dunnage from the shelving Optional Shelf Adjustment Move the shelves as needed for your application Figure 2 Shelf Installed 2 Install the right side mounting prongs first 3 Install the left side mounting prongs last 1 Tilt the shelf at rough 60 when moving up or down to avoid scarping ...

Page 18: ... your laboratory protocol clean and disinfect the cultivation chamber and shelving components prior to installation Cleaning and disinfecting reduce the risk of contamination The chamber and shelving were cleaned and disinfected at the factory however Sheldon Manufacturing cannot guarantee that the chamber was not exposed to contaminants during shipping Remove all protective wrappings from shelvin...

Page 19: ...age de la température Over Temperature Limit system Indique le système de dépassement de temperature AC Power Repère le courant alternatif Indicates I ON and O OFF I indique que l interrupteur est en position marche O indique que le commutateur est en position d arrêt Indicates protective earth ground Repère terre électrique Adjusts UP and DOWN Ajuster le haut et vers le bas Potential Shock Hazard...

Page 20: ...20 P a g e GRAPHIC SYMBOLS ...

Page 21: ...illumination and temperature phases in the cultivation chamber The EZ2 button does not have a function in the SRI chamber The green Advance button is used to scroll forward through Day Night Cycle pages and to save entered time and temperature parameter values The Reset button scrolls the display back to a previous page or menu and also saves currently selected parameter values Pressing the Reset ...

Page 22: ...splay illuminates when the day night auto cycle is active Heating Indicator The green light labeled HEATING ACTIVATED illuminates whenever power is flowing to the heating element This light will illuminate frequently during normal operations with the door closed as the heater balances the chilling effect of the refrigeration compressor to achieve a stable and uniform chamber air temperature Set Ov...

Page 23: ...e in maintaining temperature uniformity around the shelf space Manual Modes and the Day Night Auto Cycle The SRI21D comes from the factory set to operate in manual night mode which runs indefinitely with the chamber illumination lights off and at a steady state temperature A small light will turn on inside the chamber whenever the chamber door is opened The unit is set to 20 C for manual night mod...

Page 24: ...event of a failure of the main temperature microprocessor or its sensor probe by rerouting power away from the heating element Rerouting takes place whenever the temperature in the cultivation chamber exceeds the OTL setting The OTL is provided with an independent temperature probe The system is set by the end user at approximately 1 C above the current chamber temperature typically when operating...

Page 25: ...its maximum position This prevents the OTL system from interfering with setting up temperature operations 5 Perform the following procedures in the Operation section to finish preparing the chamber Set the Day Mode Temperature Set Point page 26 Set the Night Mode Temperature Set Point page 29 Program Clock Times and Auto Cycle Temperature Set Points page 32 Optional If you are required to verify t...

Page 26: ...manual day mode Continued on next page Put the unit in manual day mode 1 Verify the unit is running the manual night mode before attempting to put it in the day mode Indicator 6 near the bottom of the screen will be illuminated in the manual night mode If the auto day night cycle is running turn it off by pressing the EZ1 button If the unit is already in the manual day mode skip to step 9 to set t...

Page 27: ...rameter controls the day illumination lights located in the chamber interior Event 1 Parameter On 6 Save and advance to the Event 2 parameter a Push the Advance key once This simultaneously saves the Event 1 parameter and advances to the next parameter The small red 2 indicator will illuminate Event 2 Parameter Off 7 Change the Event 2 parameter to On a Press the Up Arrow once This parameter toggl...

Page 28: ... the homepage while operating in the day mode adjust the day temperature set point a Use the Up or Down Arrows to adjust the green set point to that of your cultivation application After the set point is adjusted there may be a pause as the unit controller calculates the optimum heating profile before heating or cooling to the new set point Note The manual mode temperature set points use memory sl...

Page 29: ...ual night mode 1 Verify the unit is running the manual day mode before attempting to place it in the night mode Indicators 2 3 and 5 will be illuminated in the manual day mode If the auto day night cycle is running turn it off by pressing the EZ1 button If the unit is already in the manual night mode skip to step 9 to set the night set point When in manual night mode the small 6 indicator near the...

Page 30: ...Up Arrow once This parameter controls the chamber day illumination lights Event 1 Parameter Off 6 Save and advance to the Event 2 parameter a Press the Advance key once This simultaneously saves the Event 1 parameter as Off while advancing The small red 2 indicator will go dark Event 2 Parameter On 7 Change the Event 2 parameter to Off a Press the Up Arrow once This parameter toggles two outputs t...

Page 31: ... homepage adjust the temperature set point with the unit operating in the manual night mode a Use the Up or Down Arrows to adjust the green set point to that of your cultivation application night temperature After the set point is adjusted there may be a pause as the unit controller calculates the optimum profile before cooling or heating to the new set point Note The manual mode temperature set p...

Page 32: ...usting it Go back a step To go back to the start of this procedure press the Reset button once Pressing the button twice at any point will return to the Home page Procedure continued on next page 12 Hour Clock 24 Hour Controller Clock 12 Hour Clock 24 Hour Controller Clock 8 00AM 08 00 2 00PM 14 00 10 00AM 10 00 6 00PM 18 00 12 00PM 12 00 8 00PM 20 00 1 00PM 13 00 12 00AM 00 00 Setting the chamber...

Page 33: ...vance button once to both save the clock setting and advance to the start of the auto cycle day phase hoU2 is the start hour of the day cycle Optional If you do not wish to adjust the day night cycle settings press the Reset button repeatedly to return to the home page after having completed Step 5a Initial Day Phase Start Hour Day phase parameters 6 Set the day phase start hour a Use the Arrow ke...

Page 34: ...ute a Use the Up and Down Arrow keys adjust the day minute 30 minutes in this example Adjusted Day Phase Start Minute 9 Save the day start minute setting and scroll to the day phase temperature set point a Press the Advance button once to save the minute setting and advance to the day temperature set point page t SP3 indicates that this is a temperature set point page reserved for the day Initial ...

Page 35: ...al Night Phase Start Hour Night phase parameters 12 Set the night phase start hour a Use the Up and Down arrow keys to adjust the night start hour Adjusted Night Phase Start Hour 13 Save the night start hour setting and scroll to the night phase start minute a Press the Advance button once to save the hour setting and advance to the night minute page Mi4 indicates the night start minute page Initi...

Page 36: ...arrow keys to adjust the set point In the display examples to the right the chamber now has a day phase temperature set point of 21 C and a night phase set point adjusted to 19 C Adjusted Night Phase Temp Set Point 17 Save the night phase set point return to the home page a Press the Advance button once to save the temperature set point at the currently displayed value and return to the home Page ...

Page 37: ...inute hoU4 New Night Phase Start Hour Mi4 Night Phase Start Minute Adjust Night Phase Start Minute Mi4 New Night Phase Start Minute t SP5 Night Phase Set Point Adjust Night Phase Temp Set Point t SP5 New Night Phase Temp Set Point 18 0 18 0 EZ1 18 0 18 0 Push Once Push Once Push Once Push Once Push Once Push Once Push Once Push Once To Set the Launch of the Day Night Auto Cycle Push Once Push Once...

Page 38: ...primarily depends on either lowering the humidity level or increasing the air temperature in the cultivation chamber Note Rising or falling air pressure from the weather will adjust the dew point up and down in small increments If the relative humidity in the incubation chamber is already near the dew point barometric fluctuations may push it across the dew point threshold Note Thin air at higher ...

Page 39: ...n end user To terminate the Cycle briefly press and hold the EZ1 button After a short pause the red graph icon will turn off The Cycle has now ended ACCESSORY COMPATIBILITY AND POWER OUTLET The SRI21D is provided with a 115 voltage 1 amp power outlet inside the chamber Do not attach powered equipment that draws more than 1 amp Verify that any powered accessory equipment used inside the chamber can...

Page 40: ...sources of heat or cold that may be adversely impacting the unit temperature stability Check if any powered accessories in the workspace chamber are generating heat If you can find no sources of external or internal temperature fluctuations contact Tech Support or your distributor for assistance End of procedure 1 Set OTL control to its maximum setting if not already set to max 2 Turn the dial cou...

Page 41: ...ts about the compatibility of decontamination or cleaning agents with the parts of the equipment or with material contained in it Warning Exercise caution if cleaning the unit with alcohol or flammable cleaners Always allow the unit to cool down to room temperature prior to cleaning and make sure all cleaning agents have evaporated or otherwise been completely removed prior to putting the unit bac...

Page 42: ...ons If possible remove all interior accessories shelves Petri dish racks and other non attached items from the chamber when disinfecting Disinfect all corners of the chamber After completion of your institutional protocol allow all disinfectants to evaporate completely MINIMIZING CONTAMINATION EXPOSURE The following are suggestions for minimizing exposure of the cultivation chamber to potential co...

Page 43: ...low freezing This happens frequently when the unit is run toward the cold end of its performance range At these temperatures humidity in the air will condense and freeze on the coil Figure 11 Refrigeration Coil and Heating Element To counteract this a defrost thermostat that checks the temperature of the coils for 20 minutes once every 12 hours If the coil surface temperature is cool enough for ic...

Page 44: ... the heat transporting coolant fluid within the coil driving an increasing loss of chilling efficiency This may even cause false high temperature readings on the defrost thermostat and prevent the system from recognizing that a defrost run is needed When this occurs the first sign of trouble is often a rise in the chamber temperature even though the main control temperature set point has not been ...

Page 45: ...ter 24 hours have elapsed 3 Close the chamber door plug the incubator power cord into the wall power source and place the unit back into operation The Low Set Limit Dial Avoiding Freezing Temps The chamber comes with a low set limit system control set at the factory to turn off the refrigeration compressor when the incubator air temperature falls to just above 0 C This is intended to prevent the i...

Page 46: ...equirements or laboratory protocol A quick reference card for the calibration procedure steps can be found on page 57 Suggested Single Point Offset Calibration Setup 3 The incubator chamber door must be closed and latched Use the tape to seal any exterior gaps caused by the probe wires 2 Place the sensor probes inside the cultivation chamber with the probe heads at least at least 2 inches 50 mm fr...

Page 47: ...ll be running the unit at during your study regardless if it will be operating in the auto cycle phases or a manual mode 2 Allow the unit to operate undisturbed for 8 hours in the manual mode at your application day temperature for example overnight before starting the calibration when first putting the unit into operation in a new environment o To be considered stabilized the unit must operate at...

Page 48: ...calibration offset must be entered to match the current display value to the reference device reading Reference Device 3 Determine the offset value and write down for use in Step 5 The offset value is the difference between the reference device reading and the reading of the chamber controller temperature display Reference Sensor Reading Controller Display Offset Value 23 0 C 22 0 C 1 0 21 2 C 22 ...

Page 49: ...wice The first push exits the Operation Options menu and saves the offset in the controller short term memory The second push returns the display to the Home Page which will now show a display value corrected with the offset to match the reference device reading The chamber will heat or chill to match the set point with the corrected display value Chamber heating to achieve the set point with the ...

Page 50: ...ce Device Chamber Display 10 If a difference still falls outside the acceptable range of your protocol repeat steps 3 11 up to two more times Three calibration attempts may be required to successfully calibrate units that are more than 2 C out of calibration If the temperature readings of the display and the reference device are still not close enough to be acceptable by your laboratory protocol a...

Page 51: ... page after approximately 5 seconds then to the Memory Management Set page after another five seconds Settings Menu Page 12 Advance to the Global Memory Parameter list a Press the Up arrow button 3 times Global Parameters List 13 Scroll to the Calibration Memory parameter a Press the green Advance button 15 times Temperature Slot Parameters 14 Select the Day Temperature Calibration Set 2 Memory sl...

Page 52: ... with the door closed This allows the unit to achieve temperature stability after the day illumination bulbs in the chamber turn off The reference device should register no temperature fluctuations of greater than 0 5 C for at least 1 hour prior to continuing Failure to wait for stabilization will result in an inaccurate display reading for the night temperature Wait 2 Hours 3 Once the temperature...

Page 53: ...The offset value will be used to adjust the controller reading to match the reference device Reference Sensor Reading Controller Display Offset Value 20 0 C 19 0 C 1 0 18 0 C 19 0 C 1 0 Reference Device Chamber Display Matching Offset Value 1 0 6 Navigate to the Operations menu page a Push and hold both the Up and Down Arrow buttons simultaneously for approximately 5 seconds until the oPEr menu ap...

Page 54: ...ce The first push exits the Operation Options menu and saves the current offset in the controller short term memory The second push returns the display to the home page now showing current display value corrected with the offset to match the reference device reading The chamber will now heat or chill to match the set point with the corrected display value Chamber cooling to achieve the set point w...

Page 55: ...Repeat steps 3 11 up to two more times for the night phase if the unit is still not calibrated Three calibration attempts may be required to successfully calibrate units that are more than 2 C out of calibration If the readings of the current temperature display and the reference device are still not close enough to be acceptable by your laboratory protocol after three calibration attempts contact...

Page 56: ...then to the Settings Menu page after another five seconds Settings Menu Page 14 Advance to the Global Settings parameter list a Press the Up arrow button 3 times Global Settings List 15 Scroll to the Memory parameter a Press the green Advance button 15 times Temperature Slot Parameters 16 Select the night temperature calibration Set 1 memory slot a Press the Up Arrow button 1 time Note Select the ...

Page 57: ...me Page Selecting the Set 2 memory slot and pressing RESET saves the current calibration offset day temp in the in the long term memory Operations Menu RESET Current Calibration Value Home Page Home Page Home Page Push and hold both buttons 10 seconds Memory Parameters Menu Global Parameters List Offset Memory Slots Day Cal Memory Slot RESET Home Page Saving the Night Calibration Continued 17 Save...

Page 58: ...nt calibration value night temp in the long term memory A1 SET 19 0 19 0 Push 3 Times LbL g SET Push 15 times Or until offset memory slots appears nonE Usr S Usr S SET 1 Push once Push Reset twice 19 0 19 0 Return to Home Page Selecting the Set 1 memory slot and pressing RESET saves the current calibration offset night in the in the long term memory Operations Menu RESET Current Calibration Value ...

Page 59: ...es the chance a service technician will be dispatched with the parts needed to fix your unit during their first visit Steps Share this information with Tech Support Read the SDRAP diagnostic questions on pages 64 and 65 and observe the unit in operation Record the observations in the SDRAP Data Log on page 60 Read the Unit Performance Specification questions on page 61 and consult the user manual ...

Page 60: ...ns SDRAP Record SDRAP Answers and Any Notes Here Set Point present setting Display present Temperature reading Reference device present reading Ambient present temperature Pilot Lights illuminating Y N Heating Indicator Refrigeration compressor running yes no Over Temperature Activated This page may be copied for institutional use ...

Page 61: ...ge 68 What is the required ambient temperature range for the unit See below under this table What is the minimum time required for your unit to come up to temperature and stabilize Allow a minimum of 4 hours of continual operation for the unit to achieve a stable temperature Standard ambient environment temperature requirements A room temperature of 15 C 30 C 59 F 86 F Standard Ventilation Spacing...

Page 62: ... answer the diagnostic questions A temperature reference device A calibrated digital thermometer with sensor probes The device must be at least as accurate as the temperature display of your unit A copy of the user manual for the unit Must be available in the same room as the unit for use ...

Page 63: ...o a power source that meets the requirements in the Installation chapter page 15 and turned on 2 A reference temperature device sensor probe must be placed in the chamber See the probe placement instructions in the Temperature Calibration procedure on page 46 3 The unit must have adequate time to come up to temperature and stabilize Failure to wait will result in an inaccurate diagnosis Allow 4 ho...

Page 64: ...rature entry in the Operation chapter of the user manual for how to display the present set point Display What temperature is presently showing in the chamber temperature display Reference What temperature is the reference device currently showing for the chamber temperature Figure 17 Set Point in Green Figure 18 Present temperature in red ...

Page 65: ...C The compressor should run continually when the unit is on Refrigeration Please listen to hear if the refrigeration compressor is running or not Note the absence of compressor noises on the SDRAP form The operation of the compressor in a noisy environment can also be detected from feeling for vibrations on the lower back of the unit Cooling issues can also be generated by a failure of the blower ...

Page 66: ...iagnoses The information is used to help ensure service techs are dispatched with the tools and parts needed to fix your unit during the first visit Facilities Technicians SDRAP and Unit Specifications data are also useful to any institutional repair technicians at your facility who may be responsible for servicing of out of warranty units End Diagnostic Data Procedure ...

Page 67: ...tandard following DIN 12880 respecting the recommended wall clearances of 10 of the height width and depth of the inner chamber All indications are average values typical for units produced in the series We reserve the right to alter technical specifications at all times WEIGHT Shipping Net Weight 381 lb 173 kg 267 0 lb 121 0 kg DIMENSIONS In inches Exterior W D H Interior W D H 36 5 x 34 5 x 79 0...

Page 68: ...lves 4 SHELF CAPACITY BY WEIGHT Per Shelf Total 20 0 lb 9 0 kg 80 0 lb 36 0 kg 20lbs 9kg with the weight evenly distributed across the shelf TEMPERATURE Temp Range Uniformity 0 to 45 C 0 5 20 C POWER Model Voltage Amperage Frequency SRI21D 115 9 0 50 60 Hz ...

Page 69: ...ling 1 800 322 4897 extension 3 If you are uncertain that you have the correct Part Number or if you need that specific part please contact Sheldon Technical Support for help at 1 800 322 4897 extension 4 or 503 640 3000 Please have the model number and serial number of the chamber ready as Tech Support will need this information to match your unit to its correct part ...

Page 70: ...P O Box 627 Cornelius OR 97113 USA support sheldonmfg com sheldonmanufacturing com 1 800 322 4897 503 640 3000 FAX 503 640 1366 ...

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