Table 6-7 Combined Method Experiments
Definition
Type
An IDA (Information Dependent Acquisition) experiment analyzes data as it is being
acquired and changes experiment conditions according to the results of the analysis.
Analysis of the results determine the masses on which to perform dependent scans. The
user has total control over the criteria that activates an IDA experiment and the parameters
of the IDA experiment that are activated.
IDA
SWATH
®
acquisition enables the MS/MS analysis of all precursor ions across a wide mass
range on an LC timescale. The Q1 quadrupole is set to a wider selection window width
(typically 10 Da to 50 Da) than that used for conventional product ion acquisitions. By
stepping through multiple, sequential selection windows, a wide mass range is covered
rapidly. The resulting mass spectra are a composite of the fragments of all of the precursor
ions that passed through the respective Q1 selection window. This technique allows for
non-targeted MS/MS analysis of all species in a sample.
SWATH
The MRM
HR
experiment helps acquire high quality MS/MS data from compounds with
known masses and retention times. This acquisition can also be used to extract fragment
masses with narrow widths (0.02 Da) from TOF MSMS spectra. The narrow extraction
gives much better selectivity.
MRM HR
A step by step procedure to guide the creation of an MRM
HR
method. After the procedure
steps are completed, the parameters are used to populate the MRM
HR
method type.
Guided MRM HR
About MS Methods
An MS method is comprised of the following elements:
• Parameters that pertain to the entire method, including
Source and Gas
parameters.
• One or more experiments.
• Each method must contain at least one experiment
• Any method can contain more than one experiment. This is referred to as looped experiments.
• The TOF MS and TOF MSMS experiments can be looped within a method, up to a maximum of 10
experiments. Q1 experiments cannot be looped.
• The IDA, SWATH, MRM
HR
experiments can be looped within a method, up to a maximum of 2 experiments.
Note:
Only specific combinations of experiments can be used, for example, IDA + IDA, SWATH + MRM
HR
.
• Each experiment has specific advanced settings.
• Individual scans within each experiment
SCIEX X500 QTOF System
System User Guide
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RUO-IDV-05-2334-E
Operating Instructions—Software
