Scientifica Multiphoton Scanhead Setup And Operation Manual Download Page 2

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© Scientifica Ltd

1A Kingfisher Court, Brambleside, Bellbrook Industrial Estate,

Uckfield, TN22 1QQ, United Kingdom

Tel +44 (0) 1825 749 933

Fax +44 (0) 1825 749 934

[email protected]

Summary of Contents for Multiphoton Scanhead

Page 1: ...Multiphoton Scanhead Setup and operation manual Revision 1 0 Manual Part No 2P Scanner MANUAL...

Page 2: ...Scientifica Ltd 1A Kingfisher Court Brambleside Bellbrook Industrial Estate Uckfield TN22 1QQ United Kingdom Tel 44 0 1825 749 933 Fax 44 0 1825 749 934 info scientifica uk com...

Page 3: ...10 3 2 Electrical setup of the Scanhead 12 3 2 1 3U Control Rack 12 3 2 2 Electrical setup 14 3 2 3 Parameters for Scanhead electrical setup 16 3 3 Optical setup of the Scanhead 17 3 3 1 Positioning t...

Page 4: ......

Page 5: ...tion of your device and may invalidate your warranty The Scanhead requires a dangerous class IV infrared pulsed laser product which produces hazardous invisible laser radiation that can permanently da...

Page 6: ...an assembly housing the X galvo scanner The input beam diameter is limited by the 3 mm 5 mm mirrors fitted to the galvanometer scanners A unique feature of the Scanhead which limits the amount of beam...

Page 7: ...used in electrophysiological work see separate SliceScope manual for further information 1 2 3 Systems components As a stand alone item the Scanhead consists of four main assemblies designed to fit o...

Page 8: ...ck aperture of the microscope objective Camera imaging port The top of the Scanhead features a camera imaging port with pre attached adapter ring this can be removed when attachment of a fluorescence...

Page 9: ...y assemblies indicated Beam expander assembly Laser input aperture SliceScope Z stage connection port Bright field illumination port Mounting points Diffusion lens lever Achromatic lens Field iris con...

Page 10: ...notify your shipping department immediately The shipping department may wish to notify the carrier at this point 2 1 Standard items Quantity Part No 1 Scanhead assembly including X Galvanometer assemb...

Page 11: ...ntensity control and beam dump Table optics for beam steering Power meter Mechanical shutter MP 4000 Launch optics and shutter Includes all relay optics to deliver the laser beam from the source into...

Page 12: ...ing List PC with monitor National Instruments 6110 PCI DAQ board Note the PCI board is pre installed into the PC Fluorescence adapter kit For fitting standard Olypmus fluorescence illuminator turret S...

Page 13: ...tents 1a CCD camera 1b CCD camera USB cable 2a Mount for tube lens and camera 2b Tube lens 3a LED intensity controller 3b LED 3c LED power supply 4 Ground glass disc 5 IR card 6 Projection lens with g...

Page 14: ...em will be shipped with red brackets to prevent component damage in transit Before proceeding remove the red shipping brackets from the base of the SliceScope using the supplied hex key set Important...

Page 15: ...the front of the SliceScope the square edges will be positioned at the rear 3 Be sure to align the four holes on the adapter bracket with the tapped holes on the beam expander assembly 4 Carefully re...

Page 16: ...with two control boards one for each galvanometer mirror As these control boards are preconfigured to be used with their respective galvanometers be sure to connect these correctly as per the labelli...

Page 17: ...or B X galvanometer board connection 9 pin D type connector supplying control signal and feedback C Y command galvo position signal input BNC connector D Y galvanometer board connection 9 pin D type c...

Page 18: ...o the 3U driver rack The Scanhead features two dual tone flying cables one driving each of the two galvanometer mirrors Connect the lower and upper of these cables to the rear panel of the large 3U ra...

Page 19: ...3 0 Initial Setup 15 Figure E Simple electrical configuration...

Page 20: ...nd data acquisition systems The driver boards that control the galvanometers can accept a position control voltage input in the range of 10 V The optical design of the Scanhead allows for maximum scan...

Page 21: ...till the alignment is finished and the beam properly terminated before re entering the lab 3 3 1 Positioning the launch optics The Launch Optics package contains the optical elements required to direc...

Page 22: ...practice to check the alignment of the galvanometers If you suspect a large misalignment of the galvanometers i e no laser beam coming through the objective please follow the procedure outlined below...

Page 23: ...u are happy with the centration of the image on the disc aperture the setscrew can be retightened Note The tube lens and scanner lenses are fixed and pre aligned in a single lens tube in the factory a...

Page 24: ...r beam 3 Close down the iris near the top periscope to a diameter approaching that of the beam waist Adjust the bottom periscope mirror mount micrometer screws to move the beam until it is centred on...

Page 25: ...prevent damage to the biological sample when not scanning it is recommended that an electro optical Pockels cell or mechanical shutter is installed in the beam path A Pockels cell has the added advan...

Page 26: ...cells of interest then laser scanning can be used to monitor fast occurring biological processes The Scanhead features a turning mirror indicated in section 1 2 3 Product walkthrough and is controlle...

Page 27: ...viceable parts within the galvo control box and galvo scanners 5 1 Cleaning WARNING Switch off and disconnect the mains supply before cleaning the unit Use a soft cloth dampened with water or a mild d...

Page 28: ...lvos are plugged into correct connectors on control box Check all other cables including drive BNC and signal connectors for damage or loose connections 6 2 Misalignment of the laser to the Scanhead I...

Page 29: ...with focus drive Galvos CT 6215 Maximum scan speed 500 Hz extended periods 1 kHz short periods 5 min Input beam diameter 3 mm Beam diameter at pupil 10 5 mm Resolution Diffraction limited Max scan ra...

Page 30: ...ach MDU 2 NI shielded cable can be connected to the 68 position connectors on either the front or rear panels whichever is the most convenient 3 Additional circuitry required across the BNC 2090A spri...

Page 31: ...sition connector 2 National Instruments BNC 2090A Shutter Control Box 3 PC Power 1 Power 1 Signal 1 BNC BNC BNC BNC BNC NI Shielded Cable NI PCI 6110 DAQ Board Signal 1 BNC Mini DIN Mini DIN X galvo S...

Page 32: ...he variable power supply rack using the Mini DIN connectors in the ports marked PMT A and PMT B Mini DIN connector 6 Connect the MDUs to the National Instruments BNC 2090A Each detection channel also...

Page 33: ...e AO0 port and the Y drive to the AO1 port on the BNC 2090A BNC cable 9 Connect the National Instruments BNC 2090A to the DAQ board pre installed in the PC Using the National Instruments shielded cabl...

Page 34: ...inal block The wiring diagram below illustrates a typical configuration for connections within the spring terminal block using a mechanical shutter connected to USER 1 Figure H Connections within Spri...

Page 35: ...ease refer to our terms and conditions of sales for information on our legal obligations However we pride ourselves on going beyond the call of duty and seek to remedy where we can and in a timely man...

Page 36: ...Scientifica Ltd 1A Kingfisher Court Brambleside Bellbrook Industrial Estate Uckfield TN22 1QQ United Kingdom Tel 44 0 1825 749 933 Fax 44 0 1825 749 934 info scientifica uk com...

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