APPLICATIONS MANUAL
A.4
Revised 10/02/2012
Procedure:
WARNING:
WEAR PROTECTIVE PERSONAL
EQUIPMENT WHEN CLEANING THE TISSUE-TEK
XPRESS X120 PRIOR TO PROCESSING TISSUES FOR
RNA STUDIES. USE OF A FUME HOOD IS
RECOMMENDED FOR PREPARATION OF THE
DECONTAMINATION SOLUTION AND THE CLEANING
OF SMALL INSTRUMENTS.
1.
Rinse all baskets and small instruments that come in
contact with the tissue using 0.1%
diethylpyrocarbonate dissolved in sterile water.
2.
Leave items in the 0.1% diethylpyrocarbonate
solution overnight.
3.
Autoclave the items in the solution for 1 hour.
4.
Wipe all parts of the instrument that come in contact
with tissue using the RNAZap wipes.
5.
Use gloves and a facemask when operating the
instrument.
Embedding of Tissue-Tek Xpress
x120 Processed Tissue
Tissue cassettes are placed into the holding chamber
of the embedding center and embedded following
laboratory protocol.
Please note that an acetic acid (vinegar) odor may
sometimes be noted upon removal of the lid from the
processed tissue cassette. This odor is present when
tissue is cut too thick for proper processing or the on-
board reagens are past their useful life.
NOTE: Improperly processed Tissue-Tek Xpress x120
and traditionally processed tissue may sometimes appear
as white areas on the embedded paraffin blocks, and/or
may show areas that appear sunken the day after
microtomy.
Microtomy
Paraffin embedded Tissue-Tek Xpress x120 tissue can
be sectioned on a Microtome. The tissue blocks usually do
not require soaking. The blocks can be faced and allowed
to cool face up on ice water for not more than 5 minutes
prior to sectioning.
CAUTION: Avoid soaking of Tissue-Tek Xpress x120-
processed paraffin blocks. Soaking may result in a
water artifact on the stained slide or in the tissue
section(s) “disintegrating” when floated on the
surface of the water.
If the paraffin ribbon fails to stretch out properly, the
waterbath temperature may need to be increased slightly
to approximately 45°- 46°C.
Compression of the ribbon may be eliminated by cool-
ing the block by spraying the paraffin block with a freezing
spray just prior to ribboning or by adjusting the knife angle.
Staining
Tissue fixed in Formalin prior to Tissue-Tek Xpress
x120 processing will stain similar to traditionally processed
tissue.
Molecular-fixed tissue will stain slightly more intensely
than Formalin-fixed tissue. The current laboratory staining
protocols may require an adjustment to the staining time
when using Hematoxylin and Eosin stains with tissue fixed
in Molecular Fixative (see “Use of Tissue-Tek Molecular
Fixative”on page A.2 for details).
Special stains performed on Tissue-Tek Xpress x120
processed tissue will appear similar to special stains
performed on traditionally processed tissue regardless of
the fixative used.
Summary of Contents for Tissue-Tek Xpress x120
Page 1: ...Operating Operating Manual Manual Rapid Tissue Processor Tissue Tek Xpress x120 ...
Page 3: ......
Page 20: ...INTRODUCTION 1 14 Revised 10 02 2012 ...
Page 24: ...INSTALLATION 2 4 Revised 10 02 2012 ...
Page 54: ...RECOVERY PROCESS 5 4 Revised 10 02 2012 ...
Page 82: ...A 8 Revised 10 02 2012 ...
